Attachment of Candida to Mammalian Tissues — Clinical and Experimental Studies

Pathogenesis of vaginal candidiasis: studies with a mutant which has reduced ability to adherein vitro

“…Lee & King [8] suggested that cell wall mannan-protein may be involved in attachment. Contrary to that, our previous studies revealed that chitin, chitin derivatives, a chitin soluble extract and amino sugars [9,12,13] blocked in-vitro adherence to human VME.Animal models have been used to study the pathogenesis of vaginal infection [9,13,15,18]. We have shown in a murine model of vaginal candidiasis that animals, pretreated with vaginal rinses consisting of a chitin-soluble extract (CSE) or N-acetyl-glucosamine, did not develop infection upon subsequent inoculation of C. albicans [9,13].…”

“…A chitin-soluble extract (CSE) prepared from 4918 blocked attachment of yeast cells to human vaginal epithelial cells, while CSE from 4918-10 did not significantly reduce the attachment of yeasts to vaginal cells. Both 4918 and 4918-10 produced hyphae in vitro and in vivo, were negative for proteinase production and grew equally well at 28"C and 37"C. The data suggest that adherence to vaginal mueosa may be an important determinant in the pathogenesis of vaginal infection eaused by C. albicans.The attachment of Candida albicans yeast cells in vitro to human vaginal mucosa epithelial (VME) cells has been described by several investigators [4,[7][8][9][12][13][14][15][16][17]. Attachment of yeast cells to human VME cells in vitro was reduced if yeast cells were killed by UV irradiation, formalin or treated with proteinases (but not pepsin).…”

Species distribution and influence of glycemic control on fungal infections in pregnant women with diabetes

“…This fact may support the hypothesis that poor glycemic control temporally precedes fungal infection. 9 In contrast, increased adherence of Candida to vaginal epithelial cells from diabetic women was related to the high glycogen concentration in these cells, 12 but adhesion of fungi did not correlate with concentration of blood glucose and HbA1 in the time of sampling. 16 The precise temporal relationship of glycemic control and mycoses is difficult to define, but when we analyse all our glucose measurements (fasting, postprandial levels), taken on the day of the specimens collection, we find no association with mycoses rate.…”

“…11 According to Segal et al Candida cells are especially adherent to epithelial cells of pregnant diabetic women. 5,12 Rurangirwa et al suggested that the inner face of the superficial layers of the stratum corneum of diabetics could be more prone to Candida invasion than that of non-diabetics. 13 In diabetic patients with vulvovaginal candidosis blood glucose level should be controlled in order to prevent future outbreakes of infection.…”

Efficiency of Lyticase (Bacterial Enzyme) in Experimental Candidal Vaginitis in Mice

“…This fact determines the importance of the search for alternative means for cleansing the microecological niche in CV. A possible approach to the solution of the problem is study of the effects of enzymes of bacterial origin modulating specifi c morphofunctional structures of C. albicans yeast-like fungi (YLF) [6].…”