Efficiency of Lyticase (Bacterial Enzyme) in Experimental Candidal Vaginitis in Mice

Sachivkina, Nadezhda; Кравцов, Э. Г.; Wasileva, E. A.; Anokchina, I. V.; Далин, М. В.

doi:10.1007/s10517-010-1037-6

Cited by 6 publications

(6 citation statements)

References 4 publications

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“…In the tables, this is indicated as five days (experience and control), and then 10 and 15 days. Prior to euthanasia, blood samples were taken via the axillary vein in all birds and were examined histopathologically ( Figure 1 b) [ 19 , 20 ].…”

Section: Methodsmentioning

confidence: 99%

Reduction in Pathogenicity in Yeast-like Fungi by Farnesol in Quail Model

Sachivkina

Vasilieva

Lenchenko

et al. 2022

Animals

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Candida albicans was the first eukaryotic microorganism to exhibit quorum-sensing through the secretion of the sesquiterpene E, farnesol. This molecule is generated by dephosphorylation of farnesyl pyrophosphate in the mevalonate biosynthetic pathway in mammalian and yeast cells. Exogenous farnesol inhibits yeast-to-hyphal formation in a concentration- and time-dependent manner at the earliest stage of hyphal development. Much research has been devoted to studying the role of farnesol as an inhibitor of hyphal morphogenesis; however, little research has been published regarding the in vivo impacts of farnesol on fungal virulence and the development of Candida infection. While other studies have examined the impact of multiple doses of farnesol in addition to antimycotics, we hypothesize that C. albicans treated with a single dose of this quorum-sensing molecule could reduce fungal virulence in a quail model.

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Section: Methodsmentioning

confidence: 99%

Reduction in Pathogenicity in Yeast-like Fungi by Farnesol in Quail Model

Sachivkina

Vasilieva

Lenchenko

et al. 2022

Animals

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“…Therefore, most of the authors believe that the cause of udder inflammation is microflora. However, some researchers emphasize the action of mechanical and chemical factors that initially cause breast injury, only after which the microbial factor begins to act [14][15][16][17][18]. Furthermore, animals with subclinical mastitis are an important and frequently neglected reservoir for the transmission of the association of microorganisms within the cattle farm or between animal herds.…”

Section: Introductionmentioning

confidence: 99%

Role of microorganisms isolated from cows with mastitis in Moscow region in biofilm formation

et al. 2021

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Background and Aim: Mastitis is one of the most important diseases of cows and the most expensive pathology for the dairy industry. Therefore, this study was conducted to explore the role of microorganisms isolated from cows with mastitis in the formation of biofilms under the conditions of farm biogeocenosis in the Moscow region. Materials and Methods: Periodic visits to 12 farms in the Moscow region were conducted to explore the microbial profile of the udder of cows with mastitis. During the visits, 103 milk samples from sick animals were collected and examined. Through microbiological analyses, 486 cultures of microorganisms were identified, which are assigned to 11 genera. Mastitis in cows is caused not only by a single pathogen but also by microbial associations, which included two to seven microbial isolates. Results: It was observed that 309 isolates (63.6%) from the total number of isolated microorganisms could form a biofilm. The ability to form biofilms was most frequently observed in Staphylococcus aureus (18.8%), Escherichia coli (11.9%), and Staphylococcus uberis (11.7%) cultures from the total number of biofilm-forming microbial cultures. Low biofilm-forming ability among the isolated microorganisms was found in lactobacilli, wherein only 20 (22.5%) Lactobacillus strains had the ability to form biofilms. The isolated microorganisms exhibited different sensitivities to antimicrobial agents, which cause difficulty in selecting an antimicrobial agent that would act on all aspects of the parasitocenosis. Conclusion: A high proportion of microorganisms isolated from cows with mastitis have the ability to form biofilms. The isolated microorganisms exhibited different and highly heterogeneous sensitivity to the action of antimicrobial drugs. This causes difficulty in using these tools for the effective control of mastitis in cows, which is frequently caused by pathogenic associations of microbial biofilms. Therefore, it is important to explore novel and more effective methods to combat this disease.

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“…As a QS molecule, farnesol participates in regulating various physiological processes in Candida , including filamentation, biofilm formation, drug susceptibility, and apoptosis [ 3 , 4 , 16 , 22 ]. This compound is produced by many organisms, mainly fungi and is also found in many essential oils of plants [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…Colonies of diurnal cultures of C. albicans from Saburo agar (Difco, France) were washed off with physiological solution (PhS; pH 7.0). The YLF concentration was 0.5 according to McFarland, corresponding to 1.5×10 8 cells/mL [ 13 - 16 ].…”

Section: Methodsmentioning

confidence: 99%

Enhancement of the antifungal activity of some antimycotics by farnesol and reduction of Candida albicans pathogenicity in a quail model experiment

et al. 2022

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Background and Aim: Clinical strains of microorganisms, including pathogenic yeast-like fungi (YLF), are resistant to currently used antifungal agents. Thus, it is relevant to study the combinations of existing antimicrobial drugs and a medicinal extract of plant origin (farnesol). In previous studies, farnesol showed a relatively strong anti-biofilm effect against Candida albicans. This study aimed to determine how much the resistance profile of non-biofilm microorganisms can change. Materials and Methods: Six clinical isolates of C. albicans and one reference strain were used to study the interaction of farnesol with the most used antimycotics. To determine the sensitivity of YLF to antimycotic drugs, such as nystatin (50 μg), amphotericin B (10 μg), ketoconazole (10 μg), clotrimazole (10 μg), voriconazole (10 μg), fluconazole (25 μg), miconazole (10 μg), and intraconazole (10 μg), the classic disk diffusion method was used. In the second stage, one of the six strains was used to simulate candidiasis of the gastrointestinal tract in an in vivo quail model. As an unusual experimental design, this study investigated the effects of pretreated C. albicans in quails, not the in vivo pathogenicity of C. albicans, after treatment with farnesol. Results: The resistance profiles of Candida strains did not improve with farnesol in all strains. All concentrations of farnesol (100, 50, and 25 μM) demonstrated a fungistatic effect (i.e., an increase in drug sensitivity) in 23 of 56 (7×8) cases (41%). The remaining 54% demonstrated no changes in the resistance to antifungal drugs or deterioration of the indicators in rare cases (5%). At 100 μM farnesol, sensitivity improved in 33 of 56 cases (59%). Candidiasis or the severity of clinical disease of the quail digestive tract developed to a lesser extent if fungi were treated with farnesol. Conclusion: Farnesol does not always show a positive result on single cells without biofilm in the laboratory. However, in a biofilm or an in vivo model with biofilms, farnesol can be considered a new antimycotic drug or an additive to existing antimycotics.

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Efficiency of Lyticase (Bacterial Enzyme) in Experimental Candidal Vaginitis in Mice

Cited by 6 publications

References 4 publications

Reduction in Pathogenicity in Yeast-like Fungi by Farnesol in Quail Model

Reduction in Pathogenicity in Yeast-like Fungi by Farnesol in Quail Model

Role of microorganisms isolated from cows with mastitis in Moscow region in biofilm formation

Enhancement of the antifungal activity of some antimycotics by farnesol and reduction of Candida albicans pathogenicity in a quail model experiment

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