Attachment of Escherichia coli O157:H7 to Abiotic Surfaces of Cooking Utensils

Abstract

We examined the attachment of enterohemorrhagic Escherichia coli O157:H7 to abiotic surfaces of cooking utensils. When the cell suspension in 0.85% NaCl (about 100 cells/mL, 10 mL) was contacted with various abiotic surfaces (square pieces, 25 cm²) at 25 °C for 20 min, the number of attached cells varied depending on the types of abiotic materials. The pathogen well attached to stainless steel (about 50 cells/25 cm²), pure titanium (35 to 45 cells/25 cm²), and glass (about 20 cells/25 cm²), but little attached to aluminum foil and plastics, irrespective of strains used. Fewer cells (below 10 cells/25 cm²) attached to stainless steel, pure titanium, and glass surfaces conditioned with aseptically sliced beef (sirloin) and autoclaved beef tallow at 25 °C for 20 min, but bovine serum albumin did not reduce the number of attached cells. The cells grown at 15 °C to the stationary phase (OD660 = about 2.8) less attached to the abiotic surfaces than those grown at 25 °C and 37 °C. When we pretreated the cells at 37 °C for 2 h with 50 μM N-hexanoyl-L-homoserine lactone (HHL), the number of cells attached to stainless steel was reduced by 70%. The number of cells attached to cooking utensils seemed to change depending on types of abiotic materials, adhesion of beef tallow to abiotic surfaces, growth temperature of the pathogen, and HHL-producing bacteria.

Effect of incubation duration, growth temperature, and abiotic surface type on cell surface properties, adhesion and pathogenicity of biofilm-detached Staphylococcus aureus cells

“…Overall, our results showed that the increase of temperature and the incubation duration slightly increased the adhesion of S. aureus to the SS and the PC. These results are in agreement with previous studies which highlighted the effect of these parameters on the adhesion of S. aureus (Abdallah et al 2014b ), Listeria monocytogenes (Gordesli and Abu-Lail 2012 ) and Escherichia coli (Tsuji and Yokoigawa 2012 ) to different surfaces. Furthermore, our results showed that biofilm-detached cells had a higher adhesion rate than that of their planktonic counterparts.…”

Impact of growth temperature on the adhesion of colistin-resistant Escherichia coli strains isolated from pigs to food-contact-surfaces

“…The change the bacterial growth temperature from 20 to 37 °C impacted the adhesion rate of E. coli cells on SS and PC. These results are in line with the previous researches which underlined the effect of the bacterial growth temperature on the adhesion of Pseudomonas aeruginosa (Abdallah et al 2014), Salmonella Enteritidis (Yang et al 2015), and E. coli (Tsuji and Yokoigawa 2012). The adhesion of cells issued from culture grown at 37 °C showed that the E. coli WT cells presented the highest adhesion rate on both surfaces.…”

Cellular, particle and environmental parameters influencing attachment in surface waters: a review

“…Temperature also has an impact on bacterial attachment by impacting cell properties and surface structures. Escherichia coli O157:H7 grown in a nutrient rich medium at 15°C to the stationary phase showed less attachment to abiotic surfaces such as stainless steel, pure titanium, glass and plastic than those grown at 25 and 37°C (Tsuji and Yokoigawa 2012). The possible reason could be E. coli cells grown at lower temperatures tend to have high motility which may suppress attachment (Turner et al 1996).…”