Impact of growth temperature on the adhesion of colistin-resistant Escherichia coli strains isolated from pigs to food-contact-surfaces

Abstract

This study investigated the effect of the growth temperature (20 and 37 °C) of Escherichia coli strains isolated from pigs on their adhesion to stainless steel and polycarbonate. This study also evaluated the ability of the DLVO and XDLVO mathematical models to predict this adhesion. The rise of growth temperature from 20 to 37 °C significantly influenced the adhesion of studied E. coli strains. The data also underlined that the mathematical prediction did not fully match with the experimental bacterial adhesion to surfaces. Furthermore, results showed that the colistin-resistant and sensitive E. coli strains adhesion depends on the type of abiotic surface. Based on these results, the mathematical models are limited in the prediction of the bacterial adhesion to abiotic surfaces. The surface roughness is a major parameter of the bacterial adhesion and should be included in the future mathematical models predicting the bacterial adhesion.

Biofilm formation by microorganisms of interest in food industries: intraspecies variability, biomarkers and control strategies based on non-thermal atmospheric plasma technologies

“…Additionally, the XPS chemical characterization revealeda relation between the abundance of oxygen polar groups (C-O, C=O, O-C=O) and the increased surface hydrophilicity. The influence of biofilm development conditions, at 37 or 12 o C, on the antibiofilm activity of the coatings was associated to the described effect of growth temperature on bacterial hydrophobicity and surface attachment (Abdallah et al, 2019;Di Bonaventura et al, 2008;Lee, Hébraud, & Bernardi, 2017). In fact, L. monocytogenes strains showed higher cellular hydrophilicity at 12 o C, conditions at which the stronger anti-biofilm activity of the best coating AP10+AA6 was observed.…”