Attachment of
            <i>Vibrio alginolyticus</i>
            to Glass Surfaces Is Dependent on Swimming Speed

Kogure, Kazuhiro; Ikemoto, Eiko; Morisaki, Hisao

doi:10.1128/jb.180.4.932-937.1998

Cited by 69 publications

(20 citation statements)

References 26 publications

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“…The attractive force between the surface and the cell in the backward mode allows the cell to stay near the surface for a long time. Kogure et al (1998) reported the positive correlation between the probability of attachment to a glass surface and the swimming speed of V. alginolyticus. The results of our The cells were assumed to swim (e) near or ( f ) far from a surface in an environment in which the point source of an attractant was set at the distance of 1 mm from the origin (X ¼ 0 mm, Y ¼ 1000 mm).…”

Section: Significance Of the Surface For Bacteriamentioning

confidence: 99%

Difference in Bacterial Motion between Forward and Backward Swimming Caused by the Wall Effect

Magariyama

Ichiba

Nakata

et al. 2005

Biophysical Journal

102

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A bacterial cell that has a single polar flagellum alternately repeats forward swimming, in which the flagellum pushes the cell body, and backward swimming, in which the flagellum pulls the cell body. We have reported that the backward swimming speeds of Vibrio alginolyticus are on average greater than the forward swimming speeds. In this study, we quantitatively measured the shape of the trajectory as well as the swimming speed. The trajectory shape in the forward mode was almost straight, whereas that in the backward mode was curved. The same parameters were measured at different distances from a surface. The difference in the motion characteristics between swimming modes was significant when a cell swam near a surface. In contrast, the difference was indistinguishable when a cell swam >60 microm away from any surfaces. In addition, a cell in backward mode tended to stay near the surface longer than a cell in forward mode. This wall effect on the bacterial motion was independent of chemical modification of the glass surface. The macroscopic behavior is numerically simulated on the basis of experimental results and the significance of the phenomenon reported here is discussed.

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Section: Significance Of the Surface For Bacteriamentioning

confidence: 99%

Difference in Bacterial Motion between Forward and Backward Swimming Caused by the Wall Effect

Magariyama

Ichiba

Nakata

et al. 2005

Biophysical Journal

102

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“…This suggests that in some instances there was migration away from stimulant over time possibly as the resource was used up. The attachment of bacteria to the bead's surface may have been a function of bacteria colliding with the surface due to their high speed [21] while swimming through the band, as the band migrated closer to the bead when resources depleted.…”

Section: ) (196 þ 12 Cells Per Screen)mentioning

confidence: 99%

Marine bacterial organisation around point-like sources of amino acids

Barbara¹,

Mitchell²

2003

FEMS Microbiology Ecology

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To better understand the trigger for and use of motility in marine bacterial chemotaxis, specific (amino acids) chemical stimuli were used to test the effect on bacterial speed and reorientation. An assay system was developed to analyse bacterial behavioural responses to point-like nutrient sources (beads 10-40 mum). The marine bacteria Pseudoalteromonas haloplanktis, Shewanella putrefaciens, Deleya marina, the enteric bacterium Escherichia coli and an enriched assemblage of marine bacteria were used in the assay. E. coli responded to the amino acids (e.g. serine, leucine) in this assay in a manner similar to the classical capillary plating method, and accumulated near the attractants using biased random walks and maximum speeds of less than 30 mum s(-1). The marine bacteria travelled more than 100 mum s(-1) and reversed direction instead of randomly tumbling to reorientate. Marine bacteria also showed different chemotactic responses. The enriched marine community and P. haloplanktis were repelled by serine while leucine was an attractant for all three marine isolates. More importantly marine bacteria formed band specific distances (>20 mum) away from individual beads. The bands were composed of free-swimming bacteria rather than bacteria attached to the beads or chamber surfaces. The bands' formation time (25-180 s) and width (5-20 mum) varied depending on the bacterial strains and attractant. This variation in chemotactic responses by the marine isolates may reflect adaptations to specific ecological niches of bacterioplankton in the ocean. However, band forming ability by marine bacteria has still only been reported in experimental studies, nutrient coupling between nutrient sources and bands of chemotactic marine bacteria have yet to be detected in the natural environment. The fragility of the bands, a clear association between free-swimming bacteria and nutrient, indicates the difficulty and potentially sets limits for finding microzones of bacteria around particles in the open ocean.

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“…Vibrio alginolyticus 138-2 that had been maintained in the Microbiology Laboratory, Ocean Research Institute at the University of Tokyo, was precultured in 1/4-ZoBell 2216E culture medium at 27°C overnight. This medium contained polypeptone (1.25 g l -1 ) and yeast extract (0.25 g l -1 ) as organic substrates with artificial seawater, which was composed of the following compounds (l -1 of distilled water [pH 7.5]): NaCl, 23.4 g; KCl, 0.8 g; MgSO 4 •7H 2 O, 4 g; CaCl 2 , 0.2 g; KBr, 100 mg; SrCl 2 •6H 2 O, 26 mg; and H 3 BO 3 , 20 mg (Kogure et al 1998). The precultured cells were separated by centrifugation and washed twice with artificial seawater to remove the 1/4-ZoBell medium.…”

mentioning

confidence: 99%

Different ingestion patterns of 13C-labeled bacteria and algae by deep-sea benthic foraminifera

Nomaki¹,

Heinz²,

Nakatsuka³

et al. 2006

Mar. Ecol. Prog. Ser.

115

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Benthic foraminiferal food sources were examined in the central part of Sagami Bay, Japan (water depth 1450 m) based on an in situ feeding experiment with 13 C-labeled food materials. In this study, 3 different 13 C-labeled food materials were used: the unicellular marine algae Dunaliella tertiolecta, the marine diatom Chaetoceros sociale, and the marine bacterium Vibrio alginolyticus. The first two are representatives of phytodetritus and the third of organic matter produced in the sediments. Each type of food material was injected into a series of in situ culture cores and incubated for up to 21 d. We observed that some benthic foraminiferal species selectively ingested 13 C-labeled algae from the sedimentary organic matter. On the other hand, benthic foraminifera ingested 13 C-labeled bacteria unselectively from sedimentary organic matter. Total benthic foraminifera assimilated 8.8 mg C m-2 d-1 of sedimentary organic matter without phytodetritus assimilation. Based on the assimilation rates estimated in this experiment, we recognized 3 types of feeding strategy among deep-sea benthic foraminifera in Sagami Bay. There are those that ingest (1) fresh phytodetritus selectively (phytophagous species: Uvigerina akitaensis, Bolivina spissa, Bolivina pacifica); (2) fresh phytodetritus selectively but sedimentary organic matter as well when phytodetritus is absent (seasonal-phytophagous species: Bulimina aculeata, Textularia kattegatensis, Globobulimina affinis); and (3) sedimentary organic matter at random (deposit feeders: Cyclammina cancellata, Chilostomella ovoidea). These different types of carbon utilization should be considered not only for understanding modern ecosystems on the deep-sea floor but also for paleoceanographic reconstructions using the abundance and distribution, or isotopic composition, of benthic foraminifera.

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Attachment of Vibrio alginolyticus to Glass Surfaces Is Dependent on Swimming Speed

Cited by 69 publications

References 26 publications

Difference in Bacterial Motion between Forward and Backward Swimming Caused by the Wall Effect

Difference in Bacterial Motion between Forward and Backward Swimming Caused by the Wall Effect

Marine bacterial organisation around point-like sources of amino acids

Different ingestion patterns of 13C-labeled bacteria and algae by deep-sea benthic foraminifera

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