Attachment of staphylococci to various synthetic polymers

Effects of Irrigation Solutions on Dentin Wettability and Roughness

“…Our study indicated that both 3% H 2 O 2 and 5.25% NaOCl significantly increased the wettability of dentin surfaces. Some groups have found that hydrophilic materials were more resistant to bacterial adhesion than hydrophobic materials (22,23). However, An et al (24) found that hydrophobic UHMWPE disks attracted much less Staphylococcus epidermidis compared with the hydrophilic metal surface.…”

Bacterial adhesion to conventional hydrogel and new silicone-hydrogel contact lens materials

“…Bioluminescence [12] is a simple and rapid method to evaluate the number of bacteria attached to surfaces [11]. It provides a very sensitive assay for ATP, with a detection limit as low as 10 −16 moles, i.e.…”

“…According to many authors, the correlation between ATP concentration and the number of bacterial cells is quite satisfactory, and the sensitivity of the method, which requires only very small quantities of reagents, makes it cost-effective [15]. Usually the number of CFU is calculated from the amount of ATP through a linear regression curve using logarithmic values of ATP and CFU [15], and quantification of cellular ATP corresponds to a valuation of cell activity [11,16]. However, inside a biofilm, not all the bacteria have the same metabolic activity, since bacterial metabolism is qualitatively modified when bacteria are fixed [5].…”

“…The bacterial adherence to polymers was assessed using a method derived from that proposed by Ludwicka et al [11]. Contact lenses were soaked in 500 μl of boiling Tris-acetate buffer (25 mM, pH 7.78 containing 0.5% (v/v) Tween 80 and EDTA 2 mM) for 1 minute in order to extract bacterial adenosyltriphosphate (ATP) and to avoid its enzymemediated degradation.…”

In situ formation of antimicrobial silver nanoparticles and the impregnation of hydrophobic polycaprolactone matrix for antimicrobial medical device applications

“…In addition, the slow water penetration and low water-sorption of these hydrophobic materials, like polycaprolactone (PCL), result in ineffective release and/or undesirable burst release of Ag. Improving the ability of hydrophobic polymers to resist infection is of great interest because bacteria are commonly found to attach more preferably to hydrophobic surfaces via hydrophobic interaction [10,11].…”