In situ formation of antimicrobial silver nanoparticles and the impregnation of hydrophobic polycaprolactone matrix for antimicrobial medical device applications

Tran, Phong A.; Hocking, Dianna M; O’Connor, Andrea J.

doi:10.1016/j.msec.2014.11.016

Cited by 58 publications

(30 citation statements)

References 20 publications

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“…7) whereas neat PCL, used as a control, showed no activity. As per previous report, the antimicrobial efficiency of hydrophobic polycaprolactone (PCL) polymer was enhanced by the incorporation of hydrophilic silver nanoparticles [32]. Adhesion of silver nanoparticles to the bacterial surface can have effect to alter their membrane properties.…”

Section: Antibacterial Activitymentioning

confidence: 57%

Electrospun Polycaprolactone Membrane Incorporated with Biosynthesized Silver Nanoparticles as Effective Wound Dressing Material

Thomas

Soumya

Mathew

et al. 2015

Appl Biochem Biotechnol

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Biosynthesized silver nanoparticles (AgNPs) incorporated polycaprolactone (PCL) nanomembrane was prepared by electrospinning as a cost-effective nanocomposite for application as an antimicrobial agent against wound infection. The nanocomposite membrane was characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) analysis and Scanning Electron microscopy (SEM). The hydrophilicity analysis of electrospun membranes as evaluated by water contact angle measurement showed the change of hydrophobicity of PCL to hydrophilic upon incorporation of silver nanoparticles. Better mechanical properties were also observed for PCL membrane due to the incorporation of silver nanoparticles and are highly supportive to explore its biomedical applications. Further antibacterial analysis of silver nanoparticle-incorporated PCL membrane against common wound pathogens coagulase-negative Staphylococcus epidermidis and Staphylococcus haemolyticus showed remarkable activity. As biosynthesized AgNPs are least explored for clinical applications, the current study is a promising cost-effective method to explore the development of silver nanoparticle-based electrospun nanocomposite to resist wound-associated infection.

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Section: Antibacterial Activitymentioning

confidence: 57%

Electrospun Polycaprolactone Membrane Incorporated with Biosynthesized Silver Nanoparticles as Effective Wound Dressing Material

Thomas

Soumya

Mathew

et al. 2015

Appl Biochem Biotechnol

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“…Incorporation of AgNPs into polymer matrices has been researched in order to obtain a prolonged bacterial inhibitory effect. Tran et al [18] fabricated hydrophobic membranes composed of PCL and PCL/polyethylene glycol with AgNPs in weight percentages of 2.6%, 3.3%, and 4.5%, achieving a constant delivering for 28 days. Silver concentration in membranes was higher than the amount released.…”

Section: Journal Of Nanomaterialsmentioning

confidence: 99%

Dose-Dependent Antimicrobial Activity of Silver Nanoparticles on Polycaprolactone Fibers against Gram-Positive and Gram-Negative Bacteria

Pazos-Ortiz

Roque-Ruiz

Hinojos-Márquez

et al. 2017

Journal of Nanomaterials

137

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The adhesion ability and adaptability of bacteria, coupled with constant use of the same bactericides, have made the increase in the diversity of treatments against infections necessary. Nanotechnology has played an important role in the search for new ways to prevent and treat infections, including the use of metallic nanoparticles with antibacterial properties. In this study, we worked on the design of a composite of silver nanoparticles (AgNPS) embedded in poly-epsilon-caprolactone nanofibers and evaluated its antimicrobial properties against various Gram-positive and Gram-negative microorganisms associated with drug-resistant infections. Polycaprolactone-silver composites (PCL-AgNPs) were prepared in two steps. The first step consisted in the reduction in situ of Ag+ions using N,N-dimethylformamide (DMF) in tetrahydrofuran (THF) solution, and the second step involved the simple addition of polycaprolactone before electrospinning process. Antibacterial activity of PCL-AgNPs nanofibers againstE. coli,S. mutans,K. pneumoniae,S. aureus,P. aeruginosa, andB. subtiliswas evaluated. Results showed sensibility ofE. coli,K. pneumoniae,S. aureus, andP. aeruginosa, but not forB. subtilisandS. mutans. This antimicrobial activity of PCL-AgNPs showed significant positive correlations associated with the dose-dependent effect. The antibacterial property of the PCL/Ag nanofibers might have high potential medical applications in drug-resistant infections.

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“…In PCL spectrum, two characteristic absorption peaks at 1,166 cm -1 and 1,726 cm -1 were related to C-O-C and C=O bonds, and peaks at 2,864 cm -1 and 2,930 cm -1 corresponded to the C-H bond. 33,38,39 After DA coating, a broad peak around 3,100-3,600 cm -1 corresponding to the stretching vibration band of phenolic hydroxyls was observed in the spectrum of PCL/DA. 13 The peak became sharper in the PCL/NS0.5, PCL/NS1.0 and PCL/NS2.0 spectra, suggesting the interaction between NS and DA.…”

Section: Characterization Of Pcl/ns Nanofibrous Meshmentioning

confidence: 99%

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

Liu¹,

Luo²,

Wang³

et al. 2017

IJN

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Bacterial infection is a major hurdle to wound healing, and the overuse of antibiotics have led to global issue, such as emergence of multidrug-resistant bacteria, even “super bacteria”. On the contrary, nanosilver (NS) can kill bacteria without causing resistant bacterial strains. In this study, NS was simply generated in situ on the polycaprolactone (PCL) nanofibrous mesh using an environmentally benign and mussel-inspired dopamine (DA). Scanning electron microscopy showed that NS uniformly formed on the nanofibers of PCL mesh. Fourier transform infrared spectroscopy revealed the step-by-step preparation of pristine PCL mesh, including DA coating and NS formation, which were further verified by water contact angle changing from hydrophobic to hydrophilic. To optimize the NS dose, the antibacterial activity of PCL/NS against Staphylococcus aureus , Escherichia coli and Acinetobacter baumannii was detected by bacterial suspension assay, and the cytotoxicity of NS was evaluated using cellular morphology observation and Cell Counting Kit-8 (CCK8) assay. Then, inductively coupled plasma atomic emission spectrometry exhibited that the optimized PCL/NS had a safe and sustained silver release. Moreover, PCL/NS could effectively inhibit bacterial infection in an infectious murine full-thickness skin wound model. As demonstrated by the enhanced level of proliferating cell nuclear antigen (PCNA) in keratinocytes and longer length of neo-formed epidermis, PCL/NS accelerated wound healing by promoting re-epithelialization via enhancing keratinocyte proliferation in infectious wounds.

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In situ formation of antimicrobial silver nanoparticles and the impregnation of hydrophobic polycaprolactone matrix for antimicrobial medical device applications

Cited by 58 publications

References 20 publications

Electrospun Polycaprolactone Membrane Incorporated with Biosynthesized Silver Nanoparticles as Effective Wound Dressing Material

Electrospun Polycaprolactone Membrane Incorporated with Biosynthesized Silver Nanoparticles as Effective Wound Dressing Material

Dose-Dependent Antimicrobial Activity of Silver Nanoparticles on Polycaprolactone Fibers against Gram-Positive and Gram-Negative Bacteria

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

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