Attempts to transfer tuberculin hypersensitivity to young rabbits

Šterzl, J.; Hrubešová, Miroslava

doi:10.1007/bf02929719

Cited by 8 publications

(3 citation statements)

References 5 publications

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“…Sterzl (8) was unsuccessful in attempts to transfer delayed hypersensitivity to neonates, but the evidence presented here indicates a very moderate passive transfer can be achieved. Serum and a suspension of spleen cells in Hanks' solution were obtained from 3 adult rabbits which had been specifically sensitized by intradermal injection of heat-killed Group A streptococcal cells.…”

Section: Effect Of Age Of the Animal--in The First Experimentsmentioning

confidence: 75%

Analysis of the Experimental Lesion of Connective Tissue Produced by a Complex of C Polysaccharide From Group a Streptococci

Schwab

1964

The Journal of Experimental Medicine

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A chronic, remittent, multinodular lesion of the connective tissue of rabbits is induced with a peptide-C polysaccharide complex from the cell walls of beta hemolytic streptococci (1). Some form of hypersensitivity is the most obvious explanation to invoke for the mechanism of this lesion, but histological, immunological, and enzymatic analyses do not support this concept (2-4).If the toxic manifestations of a bacterial component are dependent upon a mechanism of hypersensitivity, susceptibility of animals should increase with age. This is based upon the assumption that the immunological competence of the neonatal animal is not well developed, and that continued exposure to crossreactive bacterial antigens in the environment will increase the hypersensitive state of the animal as it ages. Toxicity of endotoxins from Gram-negative bacteria, for example, increases directly with age, and most of the toxic effect has an allergic basis (5).The influence of age of the animal on the chronic relapsing lesion produced by streptococcal components was studied to obtain further evidence of the role of hypersensitivity in this reaction. The age distribution of susceptibility indicates that the chronic nodular lesion involves some physiological maturation besides the immune response. The experiments were designed to follow the evolution of the allergic and of the relapsing nodular reactions, from neonatal through adult life, in rabbits sensitized by specific active or passive sensitization, or sensitized by natural contact with microbial antigens in their environment, cross-reactive with Group A streptococcal components.

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Section: Effect Of Age Of the Animal--in The First Experimentsmentioning

confidence: 75%

Analysis of the Experimental Lesion of Connective Tissue Produced by a Complex of C Polysaccharide From Group a Streptococci

Schwab

1964

The Journal of Experimental Medicine

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“…[1][2][3][4] In the present experiments, it was possible to evaluate the number of interacting cells by using a combination of recently developed techniques. Mishell and Dutton5 described a method for the induction of the primary immune response to 'sheep erythrocytes in vitro.…”

mentioning

confidence: 99%

A THREE-CELL INTERACTION REQUIRED FOR THE INDUCTION OF THE PRIMARY IMMUNE RESPONSE in vitro

Mosier

Coppleson

1968

Proc. Natl. Acad. Sci. U.S.A.

207

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The primary immune response involves the interaction of several cell types. [1][2][3][4] In the present experiments, it was possible to evaluate the number of interacting cells by using a combination of recently developed techniques. Mishell and Dutton5 described a method for the induction of the primary immune response to 'sheep erythrocytes in vitro. The numbers of antibody-forming cells in the in vitro response were enumerated by the hemolytic plaque assay described by Jerne and Nordin6 and, independently, by Ingraham and Bussard.7 In a recent paper, one of us8 has shown that cells which adhere to plastic Petri dishes and cells which do not adhere are both required for the induction of the immune response with the methods of Mishell and Dutton. Coppleson and Michie9 have shown that the number of cells interacting in a graft versus host reaction can be estimated from the slope of the log cell dose-log response line. This method of analysis was applied to the adherent and nonadherent cell populations in such a way that the number of interactions contributed by each population could be assayed separately. From these estimates, it appears that at least two, and possibly three, cell types are involved in at least two separate cell interactions during the primary immune response in vitro.Materials and Methods.-Tissue culture: Cell cultures were prepared from the spleens of 8-12-week-old DBA/2 mice. Spleen cell suspensions were prepared by teasing apart with forceps the spleens in cold Hanks' balanced salt solution (HBSS). Cell aggregates were allowed to sediment by gravity, and the resulting suspension of single cells was centrifuged for 10 min at 600 X g at 40C. The pooled sedimented cells were resuspended to a concentration of 1 X 107 cells per ml in Eagle's minimum essential medium (Grand Island Biological) supplemented with nonessential amino acids, pyruvate, and 10% fetal bovine serum (Colorado Serum). Cultures were maintained in 35-mm plastic Petri dishes (Falcon 1008) on a slowly rocking platform (Bellco) in an atmosphere of 7% 02, 10%/ C02, and 83% N2. Each culture dish received daily 50 ul of nutritional mixture8 and 50 ,ul of fetal bovine serum. These conditions closely approximate those originally described by Mishell and Dutton.5 Preparation of adherent and nonadherent cell populations: Adherent and nonadherent cells were prepared by culturing 1 X 107 spleen cells in a plastic Petri dish for 30 min on the rocking platform. Those cells remaining free in the culture medium were aspirated and designated the nonadherent population. The culture dish was then washed three times with cold HBSS, and the washings were discarded. The cells remaining firmly attached to the plastic were designated the adherent population. In each experiment all cell populations were prepared from the same pool of spleen cells.In the main series of experiments, the numbers of either adherent or nonadherent cell populations were varied, and one population was cultured in the presence of a constant excess of the other population. The...

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“…Nii võib kõne alla tulla asjaolu, et sünnijärgsel immunoloogilise areaktiivsuse perioodil puudub vastsündinu organismis metaboolne foon, mis võimaldab sisestatud DNP talitluse jätkumist. Sellele viitab mõningal määral ka J. Šterzli ja M. Hrubešovä [ 6 ] teade, et nukleoproteiididega võib ülitundlikkuse fenomeni üle kanda küll täiskasvanud, kuid mitte vastsündinud küülikule.…”

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Desoksüribonukleoproteiidi Osatähtsusest Adaptatiivses Рroteosünteesis

Pavel¹

1961

Eesti NSV Teaduste Akadeemia Toimetised. Bioloogiline Seeria

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Desoksüribonukleoproteiidi (DNP) osatähtsuse uurimiseks valgu sünteesis on kasutatud antikehade sünteesi induktiivses faasis oleva doonori põrna DNP ülekandmist antikehade sünteesivõimetule retsipiendile [ 7 > 9 > 10 j. Et antikehade sünteesivõime ülekandmisel saadud tulemused on vasturääkivad, nõuab see küsimus edasist uurimist.Käesolevas artiklis tuuakse andmeid DNP funktsiooni uurimisest kohastuslikus valgusünteesis, mis on saadud «immuunse» DNP ülekandmisel nii immunoloogiliselt areaktiivsetele kui ka reaktiivsetele retsipientidele. Materjal ja metoodikaKatseloomadena käsutati viini sinist tõugu küülikuid. Doonoreid (täiskasvanud küülikud) stimuleeriti Escherichia coli toksilise (tüvi' О 111) ja suhteliselt atoksilise (tüvi 221) tüvega, mida süstiti kõrvaveeni I-2-10 9 rakku. Kaks päeva pärast vaktsineerimist* loomad veretustati ja nende põrnadest isoleeriti DNP H. Schwanden ja R. Signeri veidi modifitseeritud meetodi [ s ] järgi. Võrreldes eelmiste töödega [ 9 . u ] lühendati nukleoproteiidide ekstraheerimise aega 1 -1 / 2 tunnile. Preparaadi P-sisaldus määrati I. Berenblumi ja E. Chaini ['] ning N-sisaldus F. Lanni jt. [ 4 ] järgi.Retsipientidena kasutati viie päeva vanuseid küülikupoegi, kolme kuni nelja kuu vanuseid küülikuid ja täiskasvanud küülikuid. Organismi aktiivse vastuse allasurumiseks DNP-preparaadis esineda võivatele E. coli antigeenidele kasutati kiiritamist röntgenikiirtega**. Kolme kuni nelja kuu vanuseid retsipiente kiiritati ca 420 r doosiga (röntgeniaparaat PYM-3, filtrid 0,5 mm Cu -j-1 mm Al, 180 kV, 15 mA, 20 r/min., fookuse kaugus looma keha keskkohast 60 cm). Täiskasvanud retsipiente kiiritati ca 600 r doosiga (kiiritamise intensiivsus 14 r/min.). Katserühma loomadele (D-rühm) süstiti esimesel või teisel päeval pärast kiiritamist 4 -5 ml DNP-lahust kõhuõõnde, kontrollrühma loomadele (О-rühm) aga E. coli vaktsiini. Retsipientidelt võeti verd südame punktsiooni teel vahetult enne preparaatide süstimist ning viiendal ja üheksandal päeval pärast preparaatide manustamist. Aglutinatsioonireaktsiooniga määrati vereseerumis O-aglutinimide olemasolu.Uurimistulemused ja arutelu Kuna «immuunse» DNP ülekandmisel saadud negatiivsed tulemused f 2 ' 3 ] võisid olla tingitud ka DNP võimalikust inaktiviseerumisest ekstraheerimise käigus, siis käesolevas töös kasutati tunduvalt lühemat ekstraheerimisaega. Samuti suurendati süstitavat DNP doosi. Nii süstiti esimeses * Bakteriaalsete preparaatide eest võlgneb autor tänu Tallinna Lastehaigla töötajale H. Lõivule.

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Attempts to transfer tuberculin hypersensitivity to young rabbits

Cited by 8 publications

References 5 publications

Analysis of the Experimental Lesion of Connective Tissue Produced by a Complex of C Polysaccharide From Group a Streptococci

Analysis of the Experimental Lesion of Connective Tissue Produced by a Complex of C Polysaccharide From Group a Streptococci

A THREE-CELL INTERACTION REQUIRED FOR THE INDUCTION OF THE PRIMARY IMMUNE RESPONSE in vitro

Desoksüribonukleoproteiidi Osatähtsusest Adaptatiivses Рroteosünteesis

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