6Once considered a uniquely human attribute, behavioral laterality has proven to be 3 7 ubiquitous among non human animals, being frequently associated with different 3 8 neurophenotypes in rodents and fish species. Zebrafish (Danio rerio) are a versatile vertebrate 3 9 model system that has been widely used in translational neuropsychiatric research due their 4 0 highly conserved genetic homology, well characterized physiological and extensive behavioral 4 1 repertoire. Although the spontaneous left-and right-bias responses and associated behavioral 4 2 domains (e.g. stress reactivity, aggression and learning) have previously been observed in other 4 3 teleost species, no information regarding how spontaneous motor left-right bias responses of 4 4 zebrafish predicts other behavioral domains has been described. Thus, we aimed to investigate 4 5 the existence and incidence of natural left-right bias of adult zebrafish in the Y-maze test and 4 6 explore any relationship of biasedness on the performance of different behavioral domains. This 4 7 included learning about threat-cues in the fear conditioning test and locomotion and anxiety-4 8related behavior in the novel tank diving test. In conclusion, we showed for the first time that 4 9 handling in a single day (see Fig. 1). During the pair-housing period, animals had shared