Attenuation of diabetic nephropathy in streptozotocin-induced diabetic rats by Punica granatum Linn. leaves extract

Mestry, Snehal N.; Dhodi, Jayesh B.; Kumbhar, Sangita Balbhim; Juvekar, Archana R.

doi:10.1016/j.jtcme.2016.06.008

Cited by 135 publications

(91 citation statements)

References 27 publications

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“…RRE and RC significantly lower renal hypertrophy in the treated diabetic rats. Increased expression of transforming growth factor beta 1 (TGF‐β1) in the glomerular mesangial and proximal convoluted tubules cells of the kidney plays a vital role in the onset and progression of renal hypertrophy (Mestry, Dhodi, Kumbhar, & Juvekar, ). RRE and RC reduced kidney index in diabetic rats.…”

Section: Discussionmentioning

confidence: 99%

“…voluted tubules cells of the kidney plays a vital role in the onset and progression of renal hypertrophy(Mestry, Dhodi, Kumbhar, & Juvekar, 2017). RRE and RC reduced kidney index in diabetic rats.Histological analysis of the kidney tissues indicated extensive damages to the kidney tissues of diabetic rats as observed by leukocytes infiltration, distorted architecture of kidney tissues.…”

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confidence: 99%

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Rhinacanthins‐rich extract and rhinacanthin C ameliorate oxidative stress and inflammation in streptozotocin‐nicotinamide‐induced diabetic nephropathy

et al. 2019

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In this present study, rhinacanthins‐rich extract (RRE) and rhinacanthin C (RC) the main bioactive constituent of Rhinacanthus nasutus was investigated for their protective effect against diabetic nephropathy (DN). Diabetes was induced by administering nicotinamide (100 mg/kg, i.p.)/streptozotocin (60 mg/kg, i.p.) and diabetic rats were orally administered with RRE and RC for 4 weeks. RRE and RC significantly reduced the kidney index, renal oxidative stress markers, and pro‐inflammatory cytokines. Furthermore, RRE and RC increased renal levels of glutathione, superoxide dismutase, catalase, and attenuated diabetic induced renal damages. In conclusion, RRE and RC confer protective effect against DN through the inhibition of oxidative stress and inflammation and could be a potential medicinal or nutritional supplement for the prevention of DN. Practical applications Rhinacanthus nasutus is a medicinal plant that is extensively used in Thai traditional medicine as an antibacterial, antifungal, antidiabetic, and anti‐inflammatory agent. The plant is rich in naphthoquinones, which confer it with several excellent bioactivities. The rich extract of the leaves was prepared with three major bioactive components and the extract was evaluated for its renoprotective effect in diabetic rats. The results from this study provides valuable pharmacological information that supports the use of the plant, especially the rich extract in the prevention and treatment of diabetes and diabetic complications.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Rhinacanthins‐rich extract and rhinacanthin C ameliorate oxidative stress and inflammation in streptozotocin‐nicotinamide‐induced diabetic nephropathy

et al. 2019

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“…Dismutation of superoxide radicals yields hydrogen peroxide, whereas Fenton reaction pathways of superoxide radicals generate hydroxyl radicals. 25,26,27,28,29,30 The elevated RONS levels are deleterious to organelles and enzymes, 31 and thereby promote injuries to body tissues. …”

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confidence: 99%

Serum Lactate Dehydrogenase Activity and Visceral Organs and Body Weights of Diabetic Rats Administered Single and Combinatorial Herbal Formulations

Chikezie¹,

Ojiako²,

Emejulu³

et al. 2018

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Background and Aim: The present study evaluated serum lactate dehydrogenase (LDH) activity. Visceral organs and body weights were used as measures of the capacities of single and combinatorial herbal formulations of Acanthus montanus, Asystasia gangetica, Gongronema latifolium and Solanum melongenas to ameliorate systemic toxicity, visceral organs inflammation or necrosis and body tissues wasting in alloxan-induced diabetes mellitus (DM) rats. Materials and Methods: Alloxan-induced DM rats with fasting plasma glucose concentration (FPGC) > 5.71 mmol/L for 5 consecutive days were selected for the study. A total of 102 male Wistar rats were divided into seventeen (17) groups of six (6) rats each. Serum LDH activity and body weights and weights of visceral organs and were measured using standard methods. Results: Serum LDH activities of herbal treated rat groups varied within a relatively narrow range of 549.9 ± 12.10 -500.6 ± 12.02 IU/L and were significantly lower (p < 0.05) than the untreated DM rat group. The body weights of the experimental rat groups after herbal treatment were significantly higher (p < 0.05) than their corresponding weights before herbal treatment. The ratios of liver weights to body weights were within the range of 0.0293 ± 1.4 x 10 -3 -0.0597 ± 2.3 x 10 -3 . The ratio of kidney weight to body weight of untreated DM rat group was 1.64 fold higher than that of normal rat group (p > 0.05). Conclusion: Overall, 200 mg/kg body weight double herbal formulations of A. gangetica + A. montanus and A. gangetica + G. latifolium offered the greatest therapeutic benefits to alloxan-induced DM rats, with respect to all diagnostic parameters considered in the present study. INTRODUCTIONLactate dehydrogenase (LDH: EC 1.1.1.27) is an oxidoreductase and cytoplasmic enzyme that catalyzes the reversible reaction: L-lactate + NAD + ↔ pyruvate + NADH. 1,2 The LDH enzyme is a tetrameric protein and is composed of five possible isoenzymes, designated as LDH -1 -LDH -5 . 1,3 Heart, kidney, brain and erythrocytes are composed of one or two of the five possible isoenzymes of LDH. 4,5 The erythrocyte cells have the highest proportion of LDH -1 and LDH -2 , 4,6 whereas the liver and skeletal muscle has the highest percentage of LDH -5 .3,7 Clinical applications of LDH activity involve quantification of one or more specific serum isoenzymes. 1,6,7,8 Accordingly, mild elevation in serum LDH activity has been reported in cases of haemolytic anemia, muscular dystrophy, pulmonary infarction, hepatitis, nephritic syndrome and cirrhosis. 9,10,11 Diabetes mellitus (DM) is multi-faceted endocrine disorder associated with array of metabolic syndromes such as hyperglycemia, dyslipidemia and impaired nitrogen balance. 12,13 A major detrimental outcome of these metabolic disorders is the generation of overwhelming cellular levels of reactive oxygen and nitrogen species (RONS), which often results in tissue and organ damage. 12,14,15 Oxidative tissue damage is mediated by activation of cellular stress-sensitive pathways, w...

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“…Creatinine, uric acid and urea are the major biomarkers utilized of renal failing. The increment in these indices are potential pathological indicators of DN development 33 . Damaging in the renal tissue was evident with the elevation of these markers in serum of diabetic animals presented in this work.…”

Section: Discussionmentioning

confidence: 99%

Rescue of Inflammatory Renal Damage by Medicinal Plant Extracts in Diabetic Rats

Saddiq¹,

Mohamed²

2019

Int J Life Sci Pharm Res

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Diabetic nephropathy (DN) is the most critical diabetic complication that leads to renal failure. The purpose of this research was to explore the anti-diabetic effectiveness of Cucumis melo var. flexuosus and/or Phoenix dactylifera fruit aqueous extracts and their mechanisms in alleviating nephropathy in hyperglycemic rats. Diabetes was promoted in rats by injection (i.p.) of a single dose of streptozotocin (STZ). C. flexuosus and P. dactylifera extracts (CFE and PDE respectively) were ingested to diabetic rats for thirty consecutive days. The data revealed that intake of either plant extract or their combination to diabetic rats, significantly diminished the serum glucose and raised the serum insulin concentration. The plant extracts significantly ameliorated the increases in relative kidney weights as well as in renal tumor necrosis factor (TNF-α), serum C -reactive protein (CRP) and renal vascular endothelial factor (VEGF). Serum kidney function markers (creatinine, uric acid and urea) were also decreased in diabetic rats treated with the plant extracts. Histopathological observation was also carried out to confirm the biochemical results. This study has proven that the current plant extracts have potential hypoglycemic effect and could attenuate diabetic inflammatory renal damage in rats. The combination of the two extracts was synergistically the effective one. This result may help in exploring a novel therapy to manage diabetes and its complications.

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Attenuation of diabetic nephropathy in streptozotocin-induced diabetic rats by Punica granatum Linn. leaves extract

Cited by 135 publications

References 27 publications

Rhinacanthins‐rich extract and rhinacanthin C ameliorate oxidative stress and inflammation in streptozotocin‐nicotinamide‐induced diabetic nephropathy

Rhinacanthins‐rich extract and rhinacanthin C ameliorate oxidative stress and inflammation in streptozotocin‐nicotinamide‐induced diabetic nephropathy

Serum Lactate Dehydrogenase Activity and Visceral Organs and Body Weights of Diabetic Rats Administered Single and Combinatorial Herbal Formulations

Rescue of Inflammatory Renal Damage by Medicinal Plant Extracts in Diabetic Rats

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