Attributes of adult stem cells

Alison, Malcolm R.; Islam, Shofiq

doi:10.1002/path.2498

Cited by 153 publications

(139 citation statements)

References 173 publications

(174 reference statements)

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“…36 Cells that coexpress ALDH and CD133 are also potent stem cells with high engraftment capacity with 65% of CD34 þ CD133 þ cells expressing additionally high levels of ALDH. 17 In contrast, cells with low ALDH have been shown to exert a healing effect following an ischemia-induced skin necrosis model suggesting endothelial progenitor cell capacity.…”

Section: Introductionmentioning

confidence: 99%

“…Higher activity of ALDH is correlated with increased tumorigenicity, resistance, metastasis potential and reduced survival. 17,44 Likewise, embryonic stem cells express markers that are shared by differentiated cells. In contrast, these very immature cells possess transcription factors (OCT 4, SOX2 and NANOG) that have been identified to be specific for embryonic stem cells.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Is it time to revisit our current hematopoietic progenitor cell quantification methods in the clinic?

Beksaç

Preffer

2011

Bone Marrow Transplant

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In the clinical practice of hematopoietic SCT, the minimum numbers of cells required for a successful engraftment are defined on the basis of their CD45 and CD34 expression profiles. However, the quantity of earlier progenitors or CD34-positive cells at different differentiation stages within stem cell grafts is not generally taken into consideration. During the last decade, various teams have quantified the number of cells expressing various combinations of CD34, CD38, CD133, CD90 co-expression and/or aldehyde dehydrogenase functional capacity using flow cytometry. Some of these studies resulted in the greater appreciation that combinations of these Ags were associated with varied myeloid, erythroid and platelet engraftment rates whereas others showed that the relative absence or presence of these markers could define cells responsible for either short-or long-term engraftment. These findings were also extended to differences between progenitor cell populations found within BM vs peripheral or cord-blood grafts. Cells harvested from donors are also generally frozen and stored; thawed cells have variable levels of viability and functional capacity based on the time tested post thaw, which also can be assessed by flow cytometry. Finally, flow cytometry has the potential for analysis of cells carrying a mesenchymal stem cell phenotype, which may be quiescent within some of the stem cell products. This review will address the need for stem cell subpopulation quantification and summarize existing published data to identify some Ags and functional characteristics that can be applicable to daily clinical practice.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Is it time to revisit our current hematopoietic progenitor cell quantification methods in the clinic?

Beksaç

Preffer

2011

Bone Marrow Transplant

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“…A defining property of MSCs is their ability to undergo adipogenic, osteogenic and chondrogenic differentiation (Horwitz et al 2005;Alison and Islam 2009). As a part of characterizing the putative spiny mouse MSCs, it was therefore necessary to assess their tri-lineage differentiation potential.…”

Section: General Growth Characteristics and Multi-lineage Differentiamentioning

confidence: 99%

The isolation and characterization of putative mesenchymal stem cells from the spiny mouse

2012

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The bone marrow represents the most common source from which to isolate mesenchymal stem cells (MSCs). MSCs are capable of differentiating into tissues of the three primary lineages and have the potential to enhance repair in damaged organs through the principals of regenerative medicine. Given the ease with which MSCs may be isolated from different species the aim of this study was to isolate and characterize putative bone marrow derived MSCs from the spiny mouse, Acomys cahirinus. MSCs were isolated from the spiny mouse in a traditional manner, and based on plastic adherence, morphology, colony forming unitfibroblast assays and functional assessment (adipogenic, osteogenic and chondrogenic differentiation potential) a population of putative mesenchymal stem cells from the compact bone of the spiny mouse have been isolated and characterized. Such methodological approaches overcome the lack of species-specific antibodies for the spiny mouse and could be employed for other species where the cost of generating species-specific antibodies is not warranted.

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“…There are two main types of stem cells considered for tissue regeneration, namely embryonic and adult stem cells. Embryonic stem cells (ESCs) are derived from the inner cell mass of blastocyst-stage embryos, whereas adult stem cells, such as mesenchymal stem cells (MSCs), can be isolated from post-natal tissues, expanded in vitro as adhesion-derived cells and induced to differentiate into multiple cell types (Alison & Islam 2009).…”

Section: Stem Cellsmentioning

confidence: 99%