Atypical changes in Candida albicans cells treated with the Venetin-1 complex from earthworm coelomic fluid

Wójcik-Mieszawska, Sylwia; Lewtak, Kinga; Sofińska-Chmiel, Weronika; Wydrych, Jerzy; Fiołka, Marta J.

doi:10.1038/s41598-023-29728-0

Cited by 2 publications

(2 citation statements)

References 64 publications

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“…It turned out that the effect was repeated and was additionally stronger than in the case of C. albicans , and the mitochondria were visible as large, elongated structures located at the edge of the cell. Enlarged mitochondria and increased production of ROS were observed in our previous studies 51 , 52 . There are data reporting that mitochondrial outer membrane proteins have been identified as mitophagy receptors in yeast 77 .…”

Section: Discussionsupporting

confidence: 61%

“…The Venetin-1 nanoparticle- a protein-polysaccharide fraction (or complex) with antifungal activity against C. albicans , was isolated from CF of the earthworm Dendrobaena veneta . Its properties and chemical character were described in earlier publications 49 – 52 . The nanoparticle has been shown to have anticancer 25 , 53 , antiaggregating 54 , and immunostimulating properties 55 .…”

Section: Introductionmentioning

confidence: 99%

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Autophagy of Candida albicans cells after the action of earthworm Venetin-1 nanoparticle with protease inhibitor activity

Wójcik-Mieszawska,

Lewtak,

Skwarek

et al. 2023

Sci Rep

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The present studies show the effect of the Venetin-1 protein-polysaccharide complex obtained from the coelomic fluid of the earthworm Dendrobaena veneta on Candida albicans cells. They are a continuation of research on the mechanisms of action, cellular targets, and modes of cell death. After the action of Venetin-1, a reduced survival rate of the yeast cells was noted. The cells were observed to be enlarged compared to the controls and deformed. In addition, an increase in the number of cells with clearly enlarged vacuoles was noted. The detected autophagy process was confirmed using differential interference contrast, fluorescence microscopy, and transmission electron microscopy. Autophagic vesicles were best visible after incubation of fungus cells with the Venetin-1 complex at a concentration of 50 and 100 µg mL−1. The changes in the vacuoles were accompanied by changes in the size of mitochondria, which is probably related to the previously documented oxidative stress. The aggregation properties of Venetin-1 were characterized. Based on the results of the zeta potential at the Venetin-1/KCl interface, the pHiep = 4 point was determined, i.e. the zeta potential becomes positive above pH = 4 and is negative below this value, which may affect the electrostatic interactions with other particles surrounding Venetin-1.

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Section: Discussionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Autophagy of Candida albicans cells after the action of earthworm Venetin-1 nanoparticle with protease inhibitor activity

Wójcik-Mieszawska,

Lewtak,

Skwarek

et al. 2023

Sci Rep

Self Cite

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The sunflower jacalin Helja: biological and structural insights of its antifungal activity against Candida albicans

Del Río,

Radicioni,

Cutine

et al. 2024

Glycobiology

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The limited availability of efficient treatments for Candida infections and the increased emergence of antifungal-resistant strains stimulates the search for new antifungal agents. We have previously isolated a sunflower mannose-binding lectin (Helja) with antifungal activity against Candida albicans, capable of binding mannose-bearing oligosaccharides exposed on the cell surface. This work aimed to investigate the biological and biophysical basis of Helja’s binding to C. albicans cell wall mannans and its influence on the fungicidal activity of the lectin. We evaluated the interaction of Helja with the cell wall mannans extracted from the isogenic parental strain (WT) and a glycosylation-defective C. albicans with altered cell wall phosphomannosylation (mnn4∆ null mutants) and investigated its antifungal effect. Helja exhibited stronger antifungal activity on the mutant strain, showing greater inhibition of fungal growth, loss of cell viability, morphological alteration, and formation of clusters with agglutinated cells. This differential biological activity of Helja was correlated with the biophysical parameters determined by solid phase assays and isothermal titration calorimetry, which demonstrated that the lectin established stronger interactions with the cell wall of the mnn4∆ null mutant than with the WT strain. In conclusion, our results provide new evidence on the nature of the Helja molecular interactions with cell wall components, i.e. phosphomannan, and its impact on the antifungal activity. This study highlights the relevance of plant lectins in the design of effective antifungal therapies.

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Atypical changes in Candida albicans cells treated with the Venetin-1 complex from earthworm coelomic fluid

Cited by 2 publications

References 64 publications

Autophagy of Candida albicans cells after the action of earthworm Venetin-1 nanoparticle with protease inhibitor activity

Autophagy of Candida albicans cells after the action of earthworm Venetin-1 nanoparticle with protease inhibitor activity

The sunflower jacalin Helja: biological and structural insights of its antifungal activity against Candida albicans

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