Atypical Switch-I Arginine plays a catalytic role in GTP hydrolysis by Rab21 from Entamoeba histolytica

Kotyada, Chaithanya; Chandra, Mintu; Tripathi, Aashutosh; Narooka, Anil Raj; Datta, Soma; Verma, Akash

doi:10.1016/j.bbrc.2018.10.113

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…They are molecular switches that present conformational changes associated with the GTP/GDP‐binding cycle. Active Rab‐GTP joins to effector proteins, whereas, inactive Rab‐GDP has low affinity for they (Kotyada et al, ; Verma et al, ).…”

Section: Introductionmentioning

confidence: 99%

EhRabB mobilises the EhCPADH complex through the actin cytoskeleton during phagocytosis of Entamoeba histolytica

Javier-Reyna

Montaño

Garcı́a-Rivera

et al. 2019

Cellular Microbiology

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Movement and phagocytosis are clue events in colonisation and invasion of tissues by Entamoeba histolytica, the protozoan causative of human amoebiasis. During phagocytosis, EhRab proteins interact with other functional molecules, conducting them to the precise cellular site. The gene encoding EhrabB is located in the complementary chain of the DNA fragment containing Ehcp112 and Ehadh genes, which encode for the proteins of the EhCPADH complex, involved in phagocytosis. This particular genetic organisation suggests that the three corresponding proteins may be functionally related. Here, we studied the relationship of EhRabB with EhCPADH and actin during phagocytosis. First, we obtained the EhRabB 3D structure to carry out docking analysis to predict the interaction sites involved in the EhRabB protein and the EhCPADH complex contact. By confocal microscopy, transmission electron microscopy, and immunoprecipitation assays, we revealed the interaction among these proteins when they move through different vesicles formed during phagocytosis. The role of the actin cytoskeleton in this event was also confirmed using Latrunculin A to interfere with actin polymerisation. This affected the movement of EhRabB and EhCPADH, as well as the rate of phagocytosis. Mutant trophozoites, silenced in EhrabB gene, evidenced the interaction of this molecule with EhCPADH and strengthened the role of actin during erythrophagocytosis.

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Section: Introductionmentioning

confidence: 99%

EhRabB mobilises the EhCPADH complex through the actin cytoskeleton during phagocytosis of Entamoeba histolytica

Javier-Reyna

Montaño

Garcı́a-Rivera

et al. 2019

Cellular Microbiology

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“…These substances are necessary for GTP hydrolysis, GTP conformational change, and GDP/GTP conversion. In the G-protein family, G2 pattern is robustly maintained but has little impact on GDP/GTP exchange activity 22 , 23 . The aim of this study was to introduce transposon nDart1-0 into highly growing local Indica rice cultivar “Basmati-370” to obtain mutant through conventional breeding approach and to characterize the ERA-like GTP-binding protein gene and its effects on the activation of phytohormones, which may be helpful to induce biotic/ abiotic stress tolerance in rice.…”

Section: Introductionmentioning

confidence: 99%

Molecular and biochemical characterization of rice developed through conventional integration of nDart1-0 transposon gene

Jalil

Ali

Khan

et al. 2023

Sci Rep

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Mutations, the genetic variations in genomic sequences, play an important role in molecular biology and biotechnology. During DNA replication or meiosis, one of the mutations is transposons or jumping genes. An indigenous transposon nDart1-0 was successfully introduced into local indica cultivar Basmati-370 from transposon-tagged line viz., GR-7895 (japonica genotype) through conventional breeding technique, successive backcrossing. Plants from segregating populationsshowed variegated phenotypes were tagged as BM-37 mutants. Blast analysis of the sequence data revealed that the GTP-binding protein, located on the BAC clone OJ1781_H11 of chromosome 5, contained an insertion of DNA transposon nDart1-0. The nDart1-0 has “A” at position 254 bp, whereas nDart1 homologs have “G”, which efficiently distinguishes nDart1-0 from its homologs. The histological analysis revealed that the chloroplast of mesophyll cells in BM-37 was disrupted with reduction in size of starch granules and higher number of osmophillic plastoglobuli, which resulted in decreased chlorophyll contents and carotenoids, gas exchange parameters (Pn, g, E, Ci), and reduced expression level of genes associated with chlorophyll biosynthesis, photosynthesis and chloroplast development. Along with the rise of GTP protein, the salicylic acid (SA) and gibberellic acid (GA) and antioxidant contents(SOD) and MDA levels significantly enhanced, while, the cytokinins (CK), ascorbate peroxidase (APX), catalase (CAT), total flavanoid contents (TFC) and total phenolic contents (TPC) significantly reduced in BM-37 mutant plants as compared with WT plants. These results support the notion that GTP-binding proteins influence the process underlying chloroplast formation. Therefore, it is anticipated that to combat biotic or abiotic stress conditions, the nDart1-0 tagged mutant (BM-37) of Basmati-370 would be beneficial.

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Atypical Switch-I Arginine plays a catalytic role in GTP hydrolysis by Rab21 from Entamoeba histolytica

Cited by 2 publications

References 17 publications

EhRabB mobilises the EhCPADH complex through the actin cytoskeleton during phagocytosis of Entamoeba histolytica

EhRabB mobilises the EhCPADH complex through the actin cytoskeleton during phagocytosis of Entamoeba histolytica

Molecular and biochemical characterization of rice developed through conventional integration of nDart1-0 transposon gene

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