Au nanoflower film-based stretchable biosensors for <i>in situ</i> monitoring of superoxide anion release in cell mechanotransduction

Zhao, Xu; Peng, Meihong; Wang, Jialu; Chen, Shutong; Lin, Yuqing

doi:10.1039/d2an01095j

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2022

DOI: 10.1039/d2an01095j

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Au nanoflower film-based stretchable biosensors for in situ monitoring of superoxide anion release in cell mechanotransduction

Xu Zhao

Meihong Peng

Jialu Wang

et al.

Abstract: Cell mechanotransduction has an important part in vascular regulation and disease development. Excessive accumulation of ROS, especially superoxide anion radicals (O2.-), is closely related to cardiovascular diseases. Lately, NADPH oxidases...

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2024

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Stressor-Actuated Proximity Labeling for Reporting Cellular Interaction

Wang,

Wang

et al. 2024

Anal. Chem.

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Cell-cell interactions determine the activation state and function of cells. When host cells are exposed to stressors such as microorganisms, immune defense machinery is activated to release H 2 O 2 , providing direct evidence of the relevant cellular physiological processes. Inspired by the fact that peroxidase can catalyze proximity labeling in the presence of exogenous H 2 O 2 , a stressor-actuated proximity labeling (SAPL) strategy is developed to report the process information on cell−cell interactions by recording stress levels. The stressors are covalently modified with horseradish peroxidase (HRP) and the H 2 O 2 released by the host cells in response to the stressors triggers HRP-based proximity labeling. Using a fungal mimic or live fungi as stressors, the stress levels of different host cells are compared by in situ imaging of the labeling signals.The ability to accumulate stress signals allows SAPL to more sensitively differentiate between interactions involving different macrophage phenotypes. SAPL is also a powerful tool for real-time, in situ monitoring of the effects of surface modifications on cellular interactions. Thus, the SAPL strategy represents a new perspective in the monitoring of cell−cell interactions using endogenous effector molecules.

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Stressor-Actuated Proximity Labeling for Reporting Cellular Interaction

Wang,

Wang

et al. 2024

Anal. Chem.

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Au nanoflower film-based stretchable biosensors for in situ monitoring of superoxide anion release in cell mechanotransduction

Abstract: Cell mechanotransduction has an important part in vascular regulation and disease development. Excessive accumulation of ROS, especially superoxide anion radicals (O2.-), is closely related to cardiovascular diseases. Lately, NADPH oxidases...

Cited by 1 publication

References 51 publications

Stressor-Actuated Proximity Labeling for Reporting Cellular Interaction

Stressor-Actuated Proximity Labeling for Reporting Cellular Interaction

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