Au-X (X=Pt/Ru)-decorated magnetic nanocubes as bifunctional nanozyme labels in colorimetric, magnetically-enhanced, one-step sandwich CRP immunoassay

Ivanova, Polina; Drozd, Marcin; Michrowski, Kamil; Karoń, Sylwia; Mazurkiewicz-Pawlicka, Marta; Pietrzak, Mariusz

doi:10.1016/j.bios.2023.115511

Cited by 10 publications

(5 citation statements)

References 48 publications

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“…Compared to the traditional sandwich method, this SA–Bio sandwich FLISA method further improves the sensitivity (∼4.25 fold) and expands the detection range, which indicates the introduction of the SA-Bio system to realize the signal amplification of sandwich FLISA. However, the LOD in this SA–Bio FLISA for CRP detection is not comparable to that of the Cd-based QD and novel Au-based FLISA. − For low-toxicity In-based QD labels, , this SA–Bio FLISA reveals the lowest LOD (Table S5).…”

Section: Resultsmentioning

confidence: 87%

Highly Sensitive, Stable InP Quantum Dot Fluorescent Probes for Quantitative Immunoassay Through Nanostructure Tailoring and Biotin–Streptavidin Coupling

Zhang,

Xu,

Zhang

et al. 2024

Inorg. Chem.

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Nontoxic, highly sensitive InP quantum dot (QD) fluorescent immunoassay probes are promising biomedical detection modalities due to their unique properties. However, InP-based QDs are prone to surface oxidation, and the stability of InP QD-based probes in biocompatible environments remains a crucial challenge. Although the thick shell can provide some protection during the phase transfer process of hydrophobic QDs, the photoluminescence quantum yield (PLQY) is generally decreased because of the contradiction between lattice stress relaxation and thick shell growth. Herein, we developed thick-shell InP-based core/shell QDs by inserting a ZnSeS alloy layer. The ternary ZnSeS intermediate shell could effectively facilitate lattice stress relaxation and passivate the defect states. The synthesized InP/ZnSe/ZnSeS/ZnS core/alloy shell/shell QDs (CAS-InP QDs) with nanostructure tailoring revealed a larger size, high PLQY (90%), and high optical stability. After amphiphilic polymer encapsulation, the aqueous CAS-InP QDs presented almost constant fluorescence attenuation and stable PL intensity under different temperatures, UV radiation, and pH solutions. The CAS-InP QDs were excellent labels of the fluorescence-linked immunosorbent assay (FLISA) for detecting C-reactive protein (CRP). The biotin–streptavidin (Bio–SA) system was first introduced in the FLISA to further improve the sensitivity, and the CAS-InP QDs-based SA–Bio sandwich FLISA realized the detection of CRP with an impressive limit of detection (LOD) of 0.83 ng/mL. It is believed that the stable and sensitive InP QD fluorescent probes will drive the rapid development of future eco-friendly, cost-effective, and sensitive in vitro diagnostic kits.

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Section: Resultsmentioning

confidence: 87%

Highly Sensitive, Stable InP Quantum Dot Fluorescent Probes for Quantitative Immunoassay Through Nanostructure Tailoring and Biotin–Streptavidin Coupling

Zhang,

Xu,

Zhang

et al. 2024

Inorg. Chem.

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“…Polina Ivanova et al synthesized noble metal-decorated magnetic nanocubes (MDMCs) for C-reactive protein (CRP) detection through a one-step sandwich NLISA. 88 This NLISA outperformed classical ELISA in sensitivity (LOD = 0.336 ng mL −1) , recovery (98.0%), and interference resistance.…”

Section: Analyte/target Selectivitymentioning

confidence: 88%

A comprehensive exploration of the latest innovations for advancements in enhancing selectivity of nanozymes for theranostic nanoplatforms

Li,

Fan,

Mei

2023

Nanoscale

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“…Among them, core–shell architectures have received significant attention in recent years due to their precise control over size, geometrical shape, and surface properties. − Although nanocomposites exhibit a higher surface-to-volume ratio, the immobilization of nanocatalysts on the magnetic carrier’s surface inevitably gives rise to leaching issues, thereby compromising the catalytic activity of the nanoenzymes . Additionally, the lack of affinity between nanocomposite enzymes and thiolated compounds renders them incompatible with conventional strategies for direct antibody effects and the affinity of immune antibodies is still in their infancy . This makes the design and synthesis of multifunctional nanocomposite enzymes as LIFA signaling sensors full of challenges.…”

Section: Introductionmentioning

confidence: 99%

“…24 Additionally, the lack of affinity between nanocomposite enzymes and thiolated compounds renders them incompatible with conventional strategies for direct antibody effects and the affinity of immune antibodies is still in their infancy. 25 This makes the design and synthesis of multifunctional nanocomposite enzymes as LIFA signaling sensors full of challenges.…”

Section: ■ Introductionmentioning

confidence: 99%

Trifunctional Nanocomposites with Colorimetric Magnetic Catalytic Activities Labels in Sandwich Immunochromatographic Detection of Escherichia coli O157:H7

Wang,

Feng,

Yan

et al. 2024

Anal. Chem.

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The emergence of nanoenzymes has catalyzed the robust advancement of the lateral flow immunoassay (LFIA) in recent years. Among them, multifunctional nanocomposite enzymes with core−shell architectures are considered preferable for promoting the sensing ability due to their good biocompatibility, precise control over size, and surface properties etc. Herein, we developed a dual-channel ensured lateral flow immunoassay (DFLIA) platform utilizing a magnetic, colorimetric, and catalytic multifunctional nanocomposite enzyme (Fe 3 O 4 @TCPP@Pd) [TCPP, Tetrakis (4-carboxyphenyl) porphyrin] for the ultrasensitive and highly accurate rapid detection of Escherichia coli O157:H7 (E. coli O157:H7). Fe 3 O 4 @TCPP@Pd-mAb exhibits superior performance compared to traditional AuNPs, including enhanced sensitivity and an extended linear detection range, benefiting from its high brightness signal, strong magnetic separation ability, and high peroxidase activity (V max = 2.32 μM S 1− ). Moreover, the Fe 3 O 4 @TCPP@Pd-labeled mAb probe exhibited exceptional stability and high affinity toward E. coli O157:H7 (with an affinity constant of approximately 1.723 × 10 9 M −1 ), indicating its potential for the efficient capture of the pathogen. Impressively, the developed Fe 3 O 4 @TCPP@Pd-DFLIA achieved ultrasensitive detection for E. coli O157:H7 with pre-and postcatalytic naked-eye detection sensitivities of 255 cfu/mL and 77 cfu/ mL, respectively, representing an approximately 41-fold improvement over the conventional AuNP-based LFIA and also possessed good specificity and reproducibility [relative standard deviation (RSD) < 10%]. Additionally, the established DFLIA exhibited satisfactory recoveries in detecting pork and milk samples, further validating the reliability of this platform for immunoassays and demonstrating its potential for utilization in bioassays and clinical diagnostics.

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Au-X (X=Pt/Ru)-decorated magnetic nanocubes as bifunctional nanozyme labels in colorimetric, magnetically-enhanced, one-step sandwich CRP immunoassay

Cited by 10 publications

References 48 publications

Highly Sensitive, Stable InP Quantum Dot Fluorescent Probes for Quantitative Immunoassay Through Nanostructure Tailoring and Biotin–Streptavidin Coupling

Highly Sensitive, Stable InP Quantum Dot Fluorescent Probes for Quantitative Immunoassay Through Nanostructure Tailoring and Biotin–Streptavidin Coupling

A comprehensive exploration of the latest innovations for advancements in enhancing selectivity of nanozymes for theranostic nanoplatforms

Trifunctional Nanocomposites with Colorimetric Magnetic Catalytic Activities Labels in Sandwich Immunochromatographic Detection of Escherichia coli O157:H7

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