Aufarbeitung von Geweben und Zellen

Chemical Composition of Enzyme‐fractionated Aged Heart Tissue

“…Two approaches have been used to isolate age pigments from cells, and both are based upon the extreme resistance of such particles to alteration during processing. Their resistance to physical disruption has been used as a basis for the isolation of pigment‐rich fractions from cardiac tissues by Lang and Siebert (4), Hendley et al (5), and Björkerud (6). We are not now concerned about the particular pigment‐like physical entities, but rather with the sum total of all those substances in the aged heart which cannot be broken down by enzymes.…”