Augmented damage of islets by impaired exocrine acinar cells undergoing apoptosis that is possibly converted to necrosis during isolation

Elgendy, Hamed; Okitsu, Teru; Kimura, Yasuko; Liu, Xibao; Nafady-Hego, Hanaa; Kurata, Jiro; Teramae, Hiroyuki; Elbahrawy, Ashraf; Üemoto, Shinji; Fukuda, Kazuhiko

doi:10.4161/isl.3.3.15611

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…Time spent in cold ischaemia is known to have a greater impact on both exocrine and endocrine pancreatic tissue than the process of cold ischaemia itself . Cold ischaemia time is negatively correlated with islet yield, islet function in vitro or post‐transplantation, revealing that preservation at 4°C is not sufficient for optimal preservation Despite the fact that a preservation solution could not be used in our conditions, we confirmed those observations using HBSS in the present study.…”

Section: Discussionsupporting

confidence: 83%

Beneficial effects of the novel marine oxygen carrier M101 during cold preservation of rat and human pancreas

Lemaire

Sigrist

Delpy³

et al. 2019

J Cellular Molecular Medi

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Ischaemia impairs organ quality during preservation in a time‐dependent manner, due to a lack of oxygen supply. Its impact on pancreas and islet transplantation outcome has been demonstrated by a correlation between cold ischaemia time and poor islet isolation efficiency. Our goal in the present study was to improve pancreas and islet quality using a novel natural oxygen carrier (M101, 2 g/L), which has been proven safe and efficient in other clinical applications, including kidney transplantation, and for several pre‐clinical transplantation models. When M101 was added to the preservation solution of rat pancreas during ischaemia, a decrease in oxidative stress (ROS), necrosis (HMGB1), and cellular stress pathway (p38 MAPK)activity was observed. Freshly isolated islets had improved function when M101 was injected in the pancreas. Additionally, human pancreases exposed to M101 for 3 hours had an increase in complex 1 mitochondrial activity, as well as activation of AKT activity, a cell survival marker. Insulin secretion was also up‐regulated for isolated islets. In summary, these results demonstrate a positive effect of the oxygen carrier M101 on rat and human pancreas during preservation, with an overall improvement in post‐isolation islet quality.

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Section: Discussionsupporting

confidence: 83%

Beneficial effects of the novel marine oxygen carrier M101 during cold preservation of rat and human pancreas

Lemaire

Sigrist

Delpy³

et al. 2019

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…There has been considerable scientific interest in the effect of islet isolation on the pancreatic tissue itself. It has been observed that during the islet isolation procedure, the exocrine acinar tissue shows elevated levels of necrosis and apoptosis . Autoactivation of pancreatic exocrine enzymes by the collagenase/protease enzyme mixture results in elevated levels of serine proteases (trypsin, chymotrypsin, and elastase) during islet isolation and in vitro culture, which correlated negatively with islet survival .…”

Section: Introductionmentioning

confidence: 99%