Auricularia auricular-judae polysaccharide attenuates lipopolysaccharide-induced acute lung injury by inhibiting oxidative stress and inflammation

Abstract

Abstract. Auricularia auricular-judae polysaccharide (AAP) has shown a variety of pharmacological properties. In the present study, the role of AAP in acute lung injury (ALI) induced by lipopolysaccharide (LPS) was analyzed in rats to further explore the possible underlying mechanisms. Adult Sprague-Dawley rats were randomly assigned into the control, AAP, LPS and LPS plus AAP groups. Rats were injected with LPS (10 mg/kg, intraperitoneal) to induce ALI. Rats in the LPS plus AAP group were treated with AAP for 7 days before the induction of ALI. The protein concentration in the bronchoalveolar lavage fluid (BALF) was measured. The animal lung edema degree was evaluated by the wet/dry (W/D) weight ratio. The myeloperoxidase (MPO) activity and malondialdehyde (MDA) level were assayed by MPO and MDA kits, respectively. The levels of inflammatory mediators, tumor necrosis factor-α (TNF-α) and interleukin (IL)-6, were assayed by the enzyme-linked immunosorbent assay method. Pathological changes of lung tissues were observed by hematoxylin and eosin staining. The data showed that treatment with AAP significantly improved LPS-induced lung pathological changes, attenuated protein concentration in the BALF, inhibited MPO activity and reduced the MDA level and lung W/D weight ratio. AAP also inhibited the release of TNF-α and IL-6 in blood. The results indicated that AAP has a protective effect on LPS-induced ALI in rats.

Honokiol protects against renal ischemia/reperfusion injury via the suppression of oxidative stress, iNOS, inflammation and STAT3 in rats

“…Previous studies have demonstrated that, in sustained diabetes, states of hyperglycemia and oxidative stress in vivo, and non-enzymatic glycation (glycosylation) reactions are evident, which are important in the pathogenesis of diabetes and nephropathy (3)(4)(5)(6). Jin et al reported that C-type natriuretic peptide ameliorates IRI-induced acute kidney injury through the inhibition of oxidative stress (7).…”

Attenuation of lipopolysaccharide-induced lung inflammation by ascorbic acid in rats: Histopathological and ultrastructural study

“…Scavengers of ROS like ascorbic acid fortunately have the ability to reduce pulmonary hypertension, hypoxia and increased microvascular permeability, which characterize ALI following infusion of endotoxin. 5,37…”

Medicinal mushroom: boon for therapeutic applications

“…Water-soluble polysaccharide (AAP I-a) isolated from Auricularia auricular prevents oxidative stress in d-galactose-induced aged mice. A paste of polysaccharides is used for the production of antiaging creams or lotions and other skin-related cosmetics in the industry (Zhang et al 2011). These polysaccharides (AAP I-a) have the ability to delay ageing as they result in the inhibition of the functional enzymes responsible for skin ageing, such as elastase, tyrosinase, hyaluronidase and MMP-1 enzymes.…”

“…1.7 -Anti-ageing Zhang et al (2011) and Hung and Nhi (2012) 12 Lentinus 26.3 65.1 2.3 -Anticancer, immunomodulatory Lovy et al (1999), Gunawardena et al (2014) and Hung and Nhi (2012) 13 Cordyceps 21.9…”