Aurora B controls kinetochore–microtubule attachments by inhibiting Ska complex–KMN network interaction

Chan, Ying Wai; Jeyaprakash, A. Arockia; Nigg, Erich A.; Santamaría, Anna

doi:10.1083/jcb.201109001

Cited by 124 publications

(153 citation statements)

References 46 publications

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“…The association between the KMN and SKA networks is negatively regulated by Aurora B activity (Chan et al, 2012). Whether phosphatases that are recruited by Knl1 regulate interactions between KMN and SKA is an open question.…”

Section: Knl1 Protein-interacting Regions Silk and Rvsf Motifsmentioning

confidence: 99%

The dynamic protein Knl1 – a kinetochore rendezvous

Ghongane

Kapanidou

Asghar³

et al. 2014

Journal of Cell Science

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Knl1 (also known as CASC5, UniProt Q8NG31) is an evolutionarily conserved scaffolding protein that is required for proper kinetochore assembly, spindle assembly checkpoint (SAC) function and chromosome congression. A number of recent reports have confirmed the prominence of Knl1 in these processes and provided molecular details and structural features that dictate Knl1 functions in higher organisms. Knl1 recruits SAC components to the kinetochore and is the substrate of certain protein kinases and phosphatases, the interplay of which ensures the exquisite regulation of the aforementioned processes. In this Commentary, we discuss the overall domain organization of Knl1 and the roles of this protein as a versatile docking platform. We present emerging roles of the protein interaction motifs present in Knl1, including the RVSF, SILK, MELT and KI motifs, and their role in the recruitment and regulation of the SAC proteins Bub1, BubR1, Bub3 and Aurora B. Finally, we explore how the regions of low structural complexity that characterize Knl1 are implicated in the cooperative interactions that mediate binding partner recognition and scaffolding activity by Knl1.

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Section: Knl1 Protein-interacting Regions Silk and Rvsf Motifsmentioning

confidence: 99%

The dynamic protein Knl1 – a kinetochore rendezvous

Ghongane

Kapanidou

Asghar³

et al. 2014

Journal of Cell Science

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“…85 Moreover, both complexes contain some components that are phosphorylated by Ipl1/Aurora B, and this phosphorylation destabilizes kinetochore-microtubule interaction. 74,75,86 Therefore, one untested possibility is that phosphorylation of the Ska complex by Aurora B also prevents SAC silencing in mammalian cells.…”

Section: Further Directionsmentioning

confidence: 99%

The current view for the silencing of the spindle assembly checkpoint

et al. 2014

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“…However, in metaphase, Kif2b is replaced by the astrin, SKAP and Ska protein complexes at kinetochores to promote kMT stability and chromosome alignment (Manning et al, 2010;Schmidt et al, 2010;Chan et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Chk1 and Mps1 jointly regulate correction of merotelic kinetochore attachments

Petsalaki

Zachos

2013

Journal of Cell Science

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SummaryIf uncorrected, merotelic kinetochore attachments can induce mis-segregated chromosomes in anaphase. We show that checkpoint kinase 1 (Chk1) protects vertebrate cells against merotelic attachments and lagging chromosomes and is required for correction of merotelic attachments during a prolonged metaphase. Decreased Chk1 activity leads to hyper-stable kinetochore microtubules, unstable binding of MCAK, Kif2b and Mps1 to centromeres or kinetochores and reduced phosphorylation of Hec1 by Aurora-B. Phosphorylation of Aurora-B at serine 331 (Ser331) by Chk1 is high in prometaphase and decreases significantly in metaphase cells. We propose that Ser331 phosphorylation is required for optimal localization of MCAK, Kif2b and Mps1 to centromeres or kinetochores and for Hec1 phosphorylation. Furthermore, inhibition of Mps1 activity diminishes initial recruitment of MCAK and Kif2b to centromeres or kinetochores, impairs Hec1 phosphorylation and exacerbates merotelic attachments in Chk1-deficient cells. We propose that Chk1 and Mps1 jointly regulate Aurora-B, MCAK, Kif2b and Hec1 to correct merotelic attachments. These results suggest a role for Chk1 and Mps1 in error correction.

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Aurora B controls kinetochore–microtubule attachments by inhibiting Ska complex–KMN network interaction

Abstract: Aurora B phosphorylation antagonizes the interaction between the Ska complex and the KMN network, thereby controlling Ska recruitment to kinetochores and stabilization of kinetochore–microtubule attachments.

Cited by 124 publications

References 46 publications

The dynamic protein Knl1 – a kinetochore rendezvous

The dynamic protein Knl1 – a kinetochore rendezvous

The current view for the silencing of the spindle assembly checkpoint

Chk1 and Mps1 jointly regulate correction of merotelic kinetochore attachments

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