Autecology of the diatom Pseudonitzschia australis, a domoic acid producer, from Monterey Bay, California

Buck, Kurt R.; Uttal-Cooke, Lisa; Pilskaln, Cynthia H.; Roelke, Daniel L.; Villac, Maria Célia; Fryxell, Greta A.; Cifuentes, Luis A.; Chávez, Francisco P.

doi:10.3354/meps084293

Cited by 125 publications

(98 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These levels agree well with previously reported values for field populations of P. multiseries in eastern Canada (up to 9 pg cell Ϫ1 ; Smith et al 1990) and of P. australis in Monterey Bay (0.4-32.6 pg cell Ϫ1 , Walz et al 1994; up to 34 pg cell Ϫ1 , Buck et al 1992; up to 75 pg cell Ϫ1 , Scholin et al 2000) and values for cultured P. multiseries from eastern Canada (up to 21 pg cell Ϫ1 ; Bates et al 1989) and P. australis isolated from Monterey Bay (37 pg cell Ϫ1 maximum, Garrison et al 1992). The cellular levels of toxin documented in Morro Bay during our cruises (up to 78 pg cell Ϫ1 ) are the highest yet recorded for a field population of P. australis.…”

Section: Discussionsupporting

confidence: 82%

“…Sinking of Pseudo-nitzschia cells-Evidence from sediment traps (Alldredge and Gottschalk 1989;Buck et al 1992) suggests that vertical export of primary production from the upper ocean to depths may occur as seasonal events following phytoplankton blooms. This vertical movement of cells through the water column may have contributed to the persistence of Pseudo-nitzschia blooms on the coast for several weeks.…”

Section: Discussionmentioning

confidence: 99%

“…The first measurements of DA in coastal regions of the U.S. were made in 1991 when high levels of toxin were found in sardines and anchovies in the Monterey Bay, California area, resulting in numerous seabird mortalities after their consumption of the fish (Buck et al 1992;Fritz et al 1992;Work et al 1993). A bloom of P. australis, identified for the first time as a species producing high levels of DA (Garrison et al 1992;Villac et al 1993), was observed in Monterey Bay during this event.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Domoic acid production near California coastal upwelling zones, June 1998

Trainer

Adams

Bill

et al. 2000

Limnology & Oceanography

179

149

View full text Add to dashboard Cite

Sea lion mortalities in central California during May and June 1998 were traced to their ingestion of sardines and anchovies that had accumulated the neurotoxin domoic acid. The detection of toxin in urine, feces, and stomach contents of several sea lions represents the first proven occurrence of domoic acid transfer through the food chain to a marine mammal. The pennate diatoms, Pseudo-nitzschia multiseries and P. australis, were the dominant, toxinproducing phytoplankton constituting algal blooms near Monterey Bay, Half Moon Bay, and Oceano Dunes, areas where sea lions with neurological symptoms stranded. Toxic Pseudo-nitzschia were also found near Morro Bay, Point Conception, Point Arguello, and Santa Barbara, demonstrating that these species were widespread along the central California coast in June 1998. Measurements of domoic acid during three cruises in early June showed the highest cellular toxin levels in P. multiseries near Point Año Nuevo at 6 pg cell Ϫ1 and in P. australis from Morro Bay at 78 pg cell Ϫ1 . Maximum cellular domoic acid levels were observed within 20 km of the coast between 0 and 5 m depth, although toxin was also measured to depths of 40 m. Hydrographic data indicated that the highest toxin levels and greatest numbers of toxic cells were positioned in water masses associated with upwelling zones near coastal headlands. Nutrient levels at these sites were less than those typically measured during periods of active upwelling, due to the 1998 El Niño event. The flow of cells and/or nutrients from coastal headlands into embayments where cells can multiply in a stratified environment is a possible mechanism of bloom development along the central California coast. This coupling of toxic Pseudo-nitzschia growth near upwelling zones with physical processes involved in cell transport will be understood only when long-term measurements are made at several key coastal locations, aiding in our capability to predict domoic-acid producing algal blooms.In May and June 1998, at least 70 California sea lions (Zalophus californianus) and one northern fur seal (Callorhinus ursinus) were found stranded along the central California coast. This stranding event occurred between 15 May 1 Corresponding author (Vera.L.Trainer@noaa.gov). AcknowledgmentsWe thank the captain and the crew of the RV Ballena, RV Shana Rae, and PB Bluefin for their help during these cruises, at times under strenuous conditions. We acknowledge the following people for their help in providing useful information that directed the locations of the cruises:

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Domoic acid production near California coastal upwelling zones, June 1998

Trainer

Adams

Bill

et al. 2000

Limnology & Oceanography

179

149

View full text Add to dashboard Cite

show abstract

“…P, pungens (strain W420Ppp2) probed with P, multiseries-specific probe. Wadden Sea (18"99 pg per cell at day 55), is comparable to quantities measured in North American isolates of this species (Bates et al, 1989) and in P. australis (Buck et al, 1992). Toxin was detected only after the stationary growth phase was reached, and not before.…”

Section: I!!i! IIsupporting

confidence: 56%

“…Other species of the Pseudonitzschia complex have also been implicated in the production of DA. P. australis Frenguelli (= P. pseudoseriata Hasle) was responsible for the death of cormorants and pelicans feeding on contaminated anchovies on the west coast of North America (Buck et al, 1992;Fritz et al, 1992;Garrison et al, 1992). Another species, P. pseudodelicatissima (Hasle) Hasle, has been linked to toxic events in the Bay of Fundy, eastern Canada (Martin et al, 1990), and has also been found throughout western Europe where it bloomed along the coast of Denmark in 1992 (Lundholm et al, 1994) and in Kiel Bay, western Baltic Sea (Hansen & Horstmann, 1993).…”

mentioning

confidence: 99%

Identification of a domoic acid-producingPseudo-nitzschiaspecies (Bacillariophyceae) in the Dutch Wadden Sea with electron microscopy and molecular probes

Vrieling

Koeman²,

Scholin

et al. 1996

European Journal of Phycology

View full text Add to dashboard Cite

Biological monitoring in the Dutch Wadden Sea between November 1993 and July 1994 revealed Pseudo-nitzschia-like pennate chain-forming diatom species with cell numbers ranging from 102 to 105 per litre. Cultured isolates and field samples were examined by electron microscopy, which revealed the majority to be Pseudo-nitzschia pungens. This species dominated over other diatoms in the phytoplankton population during November 1993 and at the end of June 1994. At the beginning of June 1994, P. fraldulenta was also present; occasionally, P. ddicatissima was observed. One isolate showed the characteristic morphology of P. multiseries. Species-specific polyclonal antibodies and large-subunit (LSU) rRNA-targeted oligonucleotides for North American strains of P. multiseries and P. pungens applied to the European isolates, confirmed species designations based on electron microscopy. The isolate of P. multiseries from the Dutch Wadden Sea produced domoic acid; after 55 days of growth about I9pg per cell was measured. This is the first report of a domoic acid-producing P. multiseries isolated from European coastal waters.

show abstract