1997
DOI: 10.1006/biol.1996.0062
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Authentication of Animal Cell Cultures by Direct Visualization of Repetitive DNA, Aldolase Gene PCR and Isoenzyme Analysis

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Cited by 22 publications
(18 citation statements)
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“…They reported simultaneous amplification of 2-4 products of various sizes, depending on the animal species analyzed. Recently Stacey et al (1997) and Liu et al (2003) followed that procedure and adapted it for certain animal cell lines of different phylogenetic relationship. The amplified products of DNA from diverse species analyzed on agarose gels resulted either in single or multiple bands with various intensities.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…They reported simultaneous amplification of 2-4 products of various sizes, depending on the animal species analyzed. Recently Stacey et al (1997) and Liu et al (2003) followed that procedure and adapted it for certain animal cell lines of different phylogenetic relationship. The amplified products of DNA from diverse species analyzed on agarose gels resulted either in single or multiple bands with various intensities.…”
Section: Resultsmentioning
confidence: 99%
“…However, only a few PCR-based methods for the identification of animals and animal cell lines have been described (Stacey et al 1997;Parodi et al 2002;Liu et al 2003;Lopez-Andreo et al 2005). Therefore, we have presented recently a preliminary report of a b-globin-based PCR focusing mainly on mouse and rat cell lines (Steube et al 2003).…”
Section: Introductionmentioning
confidence: 99%
“…However it is also imperative to routinely monitor cell lines for possible contamination using characteristics that authenticate their identity. Over recent decades several methods have been established for use in authenticating and characterising cell lines, including allozyme and isoenzyme analysis (Stacey et al 1997;O'Brien et al 1980), karyotyping analysis (Rush et al 2002), human leukocyte antigen typing (O'Toole et al 1983), immunophenotypic and immunocytochemical analysis (Quentmeier et al 2001), DNA fingerprinting and short tandem repeat profiling (Stacey et al 1992;Dirks et al 2005;Yoshino et al 2006;Azari et al 2007). In general these methodologies can be relatively time consuming and expensive as well as requiring experienced personnel to undertake them.…”
Section: Introductionmentioning
confidence: 99%
“…Yet despite the burgeoning information present in the genome databases for these species only a handful of PCR-based technologies for cell line authentification have been developed (Stacey et al 1997;Parodi et al 2002;Liu et al 2003;López-Andreo et al 2005;Steube et al 2003Steube et al , 2008. Whilst these approaches may be relatively inexpensive many of these assays utilise multiple primers or require interpretation of relatively complex gel banding patterns.…”
Section: Introductionmentioning
confidence: 99%
“…DNA fingerprinting is also useful to verify the identity and purity of the cell lines (Jeffreys et al 1985;Stacey et al 1992) and it is now used as a common method for authentication of human cell lines (Dirks et al 1999;Masters 2000). PCR based methods for the identification of animal cell lines have also been described (Stacey et al 1997;Parodi et al 2002;Liu et al 2003;Lopez-Andreo et al 2005;Losi et al 2008).…”
Section: Introductionmentioning
confidence: 99%