Author Correction: PANDORA-seq expands the repertoire of regulatory small RNAs by overcoming RNA modifications

Shi, Junchao; Zhang, Yunfang; Tan, Dongmei; Zhang, Xudong; Yan, Menghong; Zhang, Ying; Franklin, Reuben; Shahbazi, Marta; Mackinlay, Kirsty; Liu, Shichao; Kuhle, Bernhard; James, Emma; Zhang, Liwen; Qu, Yongcun; Zhai, Qiwei; Zhao, W. R.; Zhao, Linlin; Zhou, Changcheng; Gu, Weifeng; Murn, Jernej; Guo, Jingtao; Carrell, Douglas T.; Wang, Yinsheng; Cairns, Bradley R.; Yang, Xiang-Lei; Schimmel, Paul; Zernicka‐Goetz, Magdalena; Cheloufi, Sihem; Zhou, Tong; Chen, Qi

doi:10.1038/s41556-021-00687-w

Cited by 2 publications

(2 citation statements)

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“…Traditional sequencing technologies, as was also used in the present study, are not adapted to the analysis of tsRNA biogenesis with their multiple modifications, especially found at the 3′end of the tRNA fragments ( Podnar et al, 2014 ). Recent improvements in library processing technologies and workflows provide possibilities to overcome these problems ( van Dijk et al, 2019 ; Maguire et al, 2020 ; Shi et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Signature of circulating small non-coding RNAs during early fracture healing in mice

Bourgery¹,

Ekholm²,

Hiltunen³

et al. 2022

Bone Reports

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Section: Resultsmentioning

confidence: 99%

Signature of circulating small non-coding RNAs during early fracture healing in mice

Bourgery¹,

Ekholm²,

Hiltunen³

et al. 2022

Bone Reports

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“…The total RNA of the tissues for sequencing was pretreated to remove some RNA modifications. The following experiments were carried out to remove some RNA modifications that may disturb small RNA-sequencing library construction ( 28 , 29 ): 3′-aminoacyl (charged) deacylation to 3′-OH (hydroxyl group) for 3′ adaptor ligation, 3′-cP (2′, 3′-cyclic phosphate) removal to 3′-OH for 3′ adaptor ligation, 5′-OH phosphorylation to 5′-P for 5′-adaptor ligation, and N1-methyladenosine and N3-methylcytidine demethylation for efficient reverse transcription. All methods were conducted based on the rtStar tRF&tiRNA Pretreatment Kit (Arraystar, USA) protocols.…”

Section: Methodsmentioning

confidence: 99%

Altered Expression of Transfer-RNA-Derived Small RNAs in Human With Rheumatic Heart Disease

Yang

et al. 2021

Front. Cardiovasc. Med.

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Rheumatic heart disease (RHD) remains a severe public health problem in developing countries. Atrial fibrillation (AF) is a medical complication of RHD. Although the understanding of disease pathogenesis has advanced in recent years, the key questions need to be addressed. Transfer RNA–derived small RNAs (tsRNAs) are a novel type of short non-coding RNAs with potential regulatory functions in various physiological and pathological processes. The present study used tsRNAs sequencing to investigate the relationship between RHD and atrial fibrillation (AF). Three paired cardiac papillary muscles were taken from six rheumatic RHD patients with AF (3 cases) or without AF (3 cases) from January 2016 to January 2017 in Xiangya Hospital, Central South University. A total of 219 precisely matched tsRNAs were identified, and 77 tsRNAs (fold change > 2.0 and P < 0.05) were differently changed. Three tsRNAs (AS-tDR-001269, AS-tDR-001363, AS-tDR-006049) were randomly selected and confirmed by qRT-PCR. The results of qRT-PCR were consistent with tsRNAs sequencing, suggesting the tsRNAs sequencing was reliable. Subsequently, we predicted the target mRNAs of the three tsRNAs. Moreover, we verified the functions of tsRNAs targeting mRNAs in vitro. Finally, bioinformatics analysis indicated that the target genes were abundant in regulation of transcription, DNA binding, intracellular. Most of the genes were predicted to interplay with cytokine-cytokine receptor by KEGG analysis. Our findings uncover the pathological process of AF in RHD through tsRNAs sequencing. This research provides a new perspective for future research on elucidating the mechanism of AF in RHD and offers potential new candidates for the treatment and diagnosis.

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Author Correction: PANDORA-seq expands the repertoire of regulatory small RNAs by overcoming RNA modifications

Cited by 2 publications

References 0 publications

Signature of circulating small non-coding RNAs during early fracture healing in mice

Signature of circulating small non-coding RNAs during early fracture healing in mice

Altered Expression of Transfer-RNA-Derived Small RNAs in Human With Rheumatic Heart Disease

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