Author response: Mapping cortical mesoscopic networks of single spiking cortical or sub-cortical neurons

Xiao, Dongsheng; Vanni, Matthieu P.; Mitelut, Catalin; Chan, Alex Siu Wing; LeDue, Jeffrey; Xie, Yicheng; Chen, Andrew Cn; Swindale, Nicholas V.; Murphy, Timothy H.

doi:10.7554/elife.19976.035

Cited by 4 publications

(1 citation statement)

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“…A simplified 577 nm single-wavelength regression model using a coefficient of 1 at all pixels (Xiao et al, 2017) left remaining variance of 0.20 ± 0.07 in 3 Cux2-Ai140 mice, 0.21 ± 0.03 in 3 Rorb-Ai140 mice and 0.43 ± 0.03 in 5 Ntsr1-Ai140 mice ("577nm Ratiometric Demixing", figure S3). Like other models not tuned pixelwise, correction was particularly poor over large vessels, along the midline and towards the edges of the hemispheres.…”

Section: Single-wavelength Linear Regressionmentioning

confidence: 99%

Separation of hemodynamic signals from GCaMP fluorescence measured with wide-field imaging

Valley

Moore

Zhuang

et al. 2020

Journal of Neurophysiology

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Wide-field calcium imaging is often used to measure brain dynamics in behaving mice. With a large field of view and a high sampling rate, wide-field imaging can monitor activity from several distant cortical areas simultaneously, revealing cortical interactions. Interpretation of wide-field images is complicated, however, by the absorption of light by hemoglobin, which can substantially affect the measured fluorescence. One approach to separating hemodynamics and calcium signals is to use multiwavelength backscatter recordings to measure light absorption by hemoglobin. Following this approach, we develop a spatially detailed regression-based method to estimate hemodynamics. This Spatial Model is based on a linear form of the Beer–Lambert relationship but is fit at every pixel in the image and does not rely on the estimation of physical parameters. In awake mice of three transgenic lines, the Spatial Model offers improved separation of hemodynamics and changes in GCaMP fluorescence. The improvement is pronounced near blood vessels and, in contrast with the Beer–Lambert equations, can remove vascular artifacts along the sagittal midline and in general permits more accurate fluorescence-based determination of neuronal activity across the cortex. NEW & NOTEWORTHY This paper addresses a well-known and strong source of contamination in wide-field calcium-imaging data: hemodynamics. To guide researchers toward the best method to separate calcium signals from hemodynamics, we compare the performance of several methods in three commonly used mouse lines and present a novel regression model that outperforms the other techniques we consider.

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Section: Single-wavelength Linear Regressionmentioning

confidence: 99%

Separation of hemodynamic signals from GCaMP fluorescence measured with wide-field imaging

Valley

Moore

Zhuang

et al. 2020

Journal of Neurophysiology

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Mesotrode: chronic simultaneous mesoscale cortical imaging and subcortical or peripheral nerve spiking activity recording in mice

Xiao

Yan

Murphy

2023

Preprint

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Brain function originates from hierarchical spatial-temporal neural dynamics distributed across cortical and subcortical networks. However, techniques available to assess large-scale brain network activity with single-neuron resolution in behaving animals remain limited. Here we present Mesotrode that integrates chronic wide-field mesoscale cortical imaging and compact multi-site cortical/subcortical cellular electrophysiology in head-fixed mice that undergo self-initiated running or orofacial movements. Specifically, we harnessed the flexibility of chronic multi-site tetrode recordings to monitor single-neuron activity in multiple subcortical structures while simultaneously imaging the mesoscale activity of the entire dorsal cortex. A mesoscale spike-triggered averaging procedure allowed the identification of cortical activity motifs preferentially associated with single-neuron spiking. Using this approach, we were able to characterize chronic single-neuron-related functional connectivity maps for up to 60 days post-implantation. Neurons recorded from distinct subcortical structures display diverse but segregated cortical maps, suggesting that neurons of different origins participate in distinct cortico-subcortical pathways. We extended the capability of Mesotrode by implanting the micro-electrode at the facial motor nerve and found that facial nerve spiking is functionally associated with the PTA, RSP, and M2 network, and optogenetic inhibition of the PTA area significantly reduced the facial movement of the mice. These findings demonstrate that Mesotrode can be used to sample different combinations of cortico-subcortical networks over prolonged periods, generating multimodal and multi-scale network activity from a single implant, offering new insights into the neural mechanisms underlying specific behaviors.

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The sensory representation of causally controlled objects

Clancy

Mrsic-Flogel

2019

Preprint

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Intentional control over external objects is informed by our sensory experience of them, in a continuous dialogue between action and perception.How such control is represented at the sensory level, however, is not understood. Here we devised a brain machine interface (BMI) task that enabled mice to guide a visual cursor to a target location for reward, using activity in brain areas recorded with widefield calcium imaging. Parietal and higher visual cortical regions were more engaged when expert animals controlled the cursor, but not in naïve mice learning the task. Intentional control enhanced responses: single-cell recordings from parietal cortex indicated that the same visual cursor elicited larger responses when mice controlled it than when they passively viewed it. Moreover, neural responses were greater when the cursor was moving towards the target than away from it. Thus, the sensory representation of a causally-controlled object is sensitive to a subject's intention, as well as the object's instantaneous trajectory relative to the subject's goal: potentially strengthening the sensory feedback signal to adjudicating areas for exerting more fluent control.

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Author response: Mapping cortical mesoscopic networks of single spiking cortical or sub-cortical neurons

Cited by 4 publications

References 0 publications

Separation of hemodynamic signals from GCaMP fluorescence measured with wide-field imaging

Separation of hemodynamic signals from GCaMP fluorescence measured with wide-field imaging

Mesotrode: chronic simultaneous mesoscale cortical imaging and subcortical or peripheral nerve spiking activity recording in mice

The sensory representation of causally controlled objects

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