Author response: Tracking transcription factor mobility and interaction in Arabidopsis roots with fluorescence correlation spectroscopy

Clark, Natalie M; Hinde, Elizabeth; Winter, Cara M.; Fisher, Adam P; Crosti, Giuseppe; Blilou, Ikram; Gratton, Enrico; Benfey, Philip N.; Sozzani, Rosangela

doi:10.7554/elife.14770.039

Cited by 4 publications

(3 citation statements)

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“…10a). Such fusion proteins with multimeric GFPs have been employed successfully by others to restrict the mobility of mobile transcription factors 23,24 . LEC1-GFP 3 signals were observed in the endosperm nuclei at the pre-globular stage, but not in the embryos at the pre-globular, globular, heart, or linear stages (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

LEAFY COTYLEDON1 expression in the endosperm enables embryo maturation in Arabidopsis

et al. 2021

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The endosperm provides nutrients and growth regulators to the embryo during seed development. LEAFY COTYLEDON1 (LEC1) has long been known to be essential for embryo maturation. LEC1 is expressed in both the embryo and the endosperm; however, the functional relevance of the endosperm-expressed LEC1 for seed development is unclear. Here, we provide genetic and transgenic evidence demonstrating that endosperm-expressed LEC1 is necessary and sufficient for embryo maturation. We show that endosperm-synthesized LEC1 is capable of orchestrating full seed maturation in the absence of embryo-expressed LEC1. Inversely, without LEC1 expression in the endosperm, embryo development arrests even in the presence of functional LEC1 alleles in the embryo. We further reveal that LEC1 expression in the endosperm begins at the zygote stage and the LEC1 protein is then trafficked to the embryo to activate processes of seed maturation. Our findings thus establish a key role for endosperm in regulating embryo development.

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Section: Resultsmentioning

confidence: 99%

LEAFY COTYLEDON1 expression in the endosperm enables embryo maturation in Arabidopsis

et al. 2021

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“…Thus, it is considered that both SHR-mediated patterning and growth may be tightly linked in the Arabidopsis root. To date, the non-cell-autonomous function of SHR has been extensively studied ( Carlsbecker et al, 2010 ; Clark et al, 2016 ; Cui et al, 2007 ; Gallagher and Benfey, 2009 ; Gallagher et al, 2004 ; Koizumi et al, 2012a ; 2012b ; Nakajima et al, 2001 ). Nevertheless, the cell-autonomous SHR pathway, which likely exists and operates in the stele, is poorly understood.…”

Section: Resultsmentioning

confidence: 99%

SHORT-ROOT Controls Cell Elongation in the Etiolated Arabidopsis Hypocotyl

Dhar

Kim

Yoon

et al. 2022

Molecules and Cells

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Transcriptional regulation, a core component of gene regulatory networks, plays a key role in controlling individual organism's growth and development. To understand how plants modulate cellular processes for growth and development, the identification and characterization of gene regulatory networks are of importance. The SHORT-ROOT (SHR) transcription factor is known for its role in cell divisions in Arabidopsis (Arabidopsis thaliana). However, whether SHR is involved in hypocotyl cell elongation remains unknown. Here, we reveal that SHR controls hypocotyl cell elongation via the transcriptional regulation of XTH18, XTH22, and XTH24, which encode cell wall remodeling enzymes called xyloglucan endotransglucosylase/hydrolases (XTHs). Interestingly, SHR activates transcription of the XTH genes, independently of its partner SCARECROW (SCR), which is different from the known mode of action. In addition, overexpression of the XTH genes can promote cell elongation in the etiolated hypocotyl. Moreover, confinement of SHR protein in the stele still induces cell elongation, despite the aberrant organization in the hypocotyl ground tissue. Therefore, it is likely that SHR-mediated growth is uncoupled from SHR-mediated radial patterning in the etiolated hypocotyl. Our findings also suggest that intertissue communication between stele and endodermis plays a role in coordinating hypocotyl cell elongation of the Arabidopsis seedling. Taken together, our study identifies SHR as a new crucial regulator that is necessary for cell elongation in the etiolated hypocotyl.

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“…B 285: 20181746 [24 -27], whereas SCR is expressed in the CEI, CEID and endodermis, and sequesters SHR to the nucleus in those cells [22,24]. In the CEID, SHR directly activates SCR, increasing its expression and regulating the periclinal division of the CEID [28][29][30][31] (figure 2). Once that SCR sequesters SHR to the nucleus, they form an active transcriptional complex and together they regulate CEID division promoting the expression of CYCLIND6;1 (CYCD6;1) [29,30].…”

Section: (A) a Genetic Network Controls Endodermis And Cortex Patterningmentioning

confidence: 99%

Building the differences: a case for the ground tissue patterning in plants

2018

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A key question in biology is to understand how interspecies morphological diversities originate. Plant roots present a huge interspecific phenotypical variability, mostly because roots largely contribute to adaptation to different kinds of soils. One example is the interspecific cortex layer number variability, spanning from one to several. Here, we review the latest advances in the understanding of the mechanisms expanding and/or restricting cortical layer number in Arabidopsis thaliana and their involvement in cortex pattern variability among multi-cortical layered species such as Cardamine hirsuta or Oryza sativa .

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Author response: Tracking transcription factor mobility and interaction in Arabidopsis roots with fluorescence correlation spectroscopy

Cited by 4 publications

References 0 publications

LEAFY COTYLEDON1 expression in the endosperm enables embryo maturation in Arabidopsis

LEAFY COTYLEDON1 expression in the endosperm enables embryo maturation in Arabidopsis

SHORT-ROOT Controls Cell Elongation in the Etiolated Arabidopsis Hypocotyl

Building the differences: a case for the ground tissue patterning in plants

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