The precise recognition of multi-hand signs in real-time under dynamic backgrounds, illumination conditions is a time consuming process. In this paper, a time efficient single stage convolutional neural network (CNN) You Only Look Once (YOLO-V2) model is proposed for real-time multi-hand sign recognition. The model utilizes DarkNet-19 CNN architecture as a feature extractor. The model is trained and tested on three distinct datasets (NUSHP-II, SENZ-3D and MITI-HD). The range of IoU from 0.5 to 0.95, the model is validated using test dataset. For the MITI-HD, the YOLO-V2 CNN model obtained an average precision value of 99.10% for AP 0.5 ; 93.00% for AP 0.75 and 78.30% for AP 0.5:0.95 . The Adam Optimizer on YOLO-V2 CNN model supersedes the other optimization methods. The prediction time of YOLO-V2 CNN is obtained as 20 ms, much lower than other single-stage hand sign recognition systems.