Autoclaved spent substrate of hatakeshimeji mushroom (Lyophyllum decastes Sing.) and its water extract protect cucumber from anthracnose

Parada, Roxana Y.; Murakami, Shigeyuki; Shimomura, Norihiro; Egusa, Mayumi; Otani, Hiroshi

doi:10.1016/j.cropro.2010.11.021

Cited by 18 publications

(28 citation statements)

References 35 publications

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“…In a previous work, we reported that treatment of the first true leaves of cucumber with AWESMS from hatakeshimeji systemically protected cucumber plants against anthracnose without antifungal activity against spore germination and mycelia growth of the pathogen (Parada et al. 2011).…”

Section: Discussionmentioning

confidence: 99%

“…For long storage, the AWESMS was divided in 250‐ml bottles containing 100 ml of the extract, again autoclaved at 121°C for 5 min and kept at 4°C until use. The ASMS of hatakeshimeji and eringi was prepared as described by Parada et al. (2011).…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, fresh SMS that is still impregnated with mushroom mycelia could be a viable and abundant source of elicitors for SAR. We recently showed that the autoclaved SMS (ASMS) of Lyophyllum decastes (hatakeshimeji) and autoclaved water extract from spent mushroom substrate (AWESMS) protect cucumber ( Cucumis sativus L.) against anthracnose ( Colletotrichum orbiculare ), and the protective effect may be associated with SAR activated by elicitors in SMS (Parada et al. 2011).…”

Section: Introductionmentioning

confidence: 99%

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Suppression of Fungal and Bacterial Diseases of Cucumber Plants by Using the Spent Mushroom Substrate of Lyophyllum decastes and Pleurotus eryngii

Parada

Murakami²,

Shimomura

et al. 2012

Journal of Phytopathology

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The protective effect of autoclaved water extract from spent mushroom substrate (AWESMS) and autoclaved spent mushroom substrate (ASMS) of the edible mushrooms Lyophyllum decastes (hatakeshimeji) and Pleurotus eryngii (eringi) against fungal and bacterial diseases was investigated on cucumber plants. When the plants were treated with AWESMS by spraying the first true leaves and inoculated with the target pathogen 7 days later, AWESMS of hatakeshimeji significantly reduced powdery mildew by Podosphaera xanthii, angular leaf spot by Pseudomonas syringae pv. lachrymans, but not Corynespora leaf spot by Corynespora cassiicola and scab by Cladosporium cucumerinum. When the plants were grown in a mixture (1 : 2, v/v) of ASMS of hatakeshimeji and soil, a significant disease reduction was observed in powdery mildew, scab and angular leaf spot. The protective effect was also observed against anthracnose on plants treated with AWESMS or on plants grown in a mixture of ASMS of eringi (1 : 3, v/v). Our results indicated that AWESMS and ASMS, independently of the mushroom type, provide a protective effect against fungal and bacterial diseases. Therefore, SMS should be considered an easily available source of active compounds to protect plants from fungal and bacteria infections, helping alleviate the waste disposal problem in the mushroom industry and creating an environmentally friendly method to reduce plant pathogens.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Suppression of Fungal and Bacterial Diseases of Cucumber Plants by Using the Spent Mushroom Substrate of Lyophyllum decastes and Pleurotus eryngii

Parada

Murakami²,

Shimomura

et al. 2012

Journal of Phytopathology

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“…Heat treatments by autoclaving, acid hydrolysis, or enzymatic treatment are often used to release elicitors from plant or fungal material, although this is not always essential [22]. With the L. perenne elicitor, autoclaving the supernatant or pellet fractions neither increased nor decreased the elicitor activity (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, paper pulp and mushroom production is not seasonal, whilst olive processing is mostly carried out in the winter months. Regardless of this, by-products from these three industries have been successfully tested for their uses in crop production [22,[33][34][35][36][37].…”

Section: Discussionmentioning

confidence: 99%

Bio-refining of perennial ryegrass (Lolium perenne): evaluation of aqueous extracts for plant defence elicitor activity using French bean cell suspension cultures

Selby

Carmichael

Sharma

2016

Chem. Biol. Technol. Agric.

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Background: There is growing interest in the bio-refining of foliage grasses to yield a range of industrial raw products. The aim of this research was to evaluate if aqueous extracts from grasses have the potential to act as crop biostimulants by assessing their defence elicitor activity.Results: Field-grown foliage (500 g fresh weight) from the Lolium perenne varieties Tyrella, Dromara, Abermagic, and Spelga were homogenised in water (2 l) and the run-off liquid filtered through Whatman No. 1 filter paper before freezing. Thawed filtrate was centrifuged at 21,000 rpm for 15 min, to give pellet and supernatant fractions, which were assayed separately for defence elicitor activity by adding them to cell suspension cultures of Phaseolus vulgaris in the early exponential phase of growth (7-10 days into a 14 days culture passage). Elicitor activity was evaluated 24 h after treatment by assessing the extent of necrosis (browning) in the P. vulgaris cells. Supernatant and pellet fractions of all four L. perenne varieties were found to have potent cell defence elicitor activity, although there were relatively small but significant differences between the varieties. In a second series of extractions, homogenisation of tissues in water was compared with screw-pressed juice using the varieties, Malone, Seagoe, and Copeland. Varietal differences in elicitor activity of these extracts were removed if they were first equalised for protein content prior to eliciting the bean cells, although the screw-pressed juice was marginally more active as an elicitor than the homogenised extract. Autoclaving the extracts in attempts to solubilise/release additional elicitor compounds neither increased nor decreased the elicitor activities of the supernatant or pellet fractions. Elicitor activity of the extract was partially reduced by treating with a cation exchange resin, almost entirely removed by adsorption onto activated carbon and completely abolished by ashing at 650 °C. Conclusion:The L. perenne defence elicitor(s) is therefore organic rather than inorganic, heat-stable, cationic at neutral pH, and readily soluble in water at room temperature.

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Spent Mushroom Substrate Uses

Rinker

2017

Edible and Medicinal Mushrooms

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Autoclaved spent substrate of hatakeshimeji mushroom (Lyophyllum decastes Sing.) and its water extract protect cucumber from anthracnose

Cited by 18 publications

References 35 publications

Suppression of Fungal and Bacterial Diseases of Cucumber Plants by Using the Spent Mushroom Substrate of Lyophyllum decastes and Pleurotus eryngii

Suppression of Fungal and Bacterial Diseases of Cucumber Plants by Using the Spent Mushroom Substrate of Lyophyllum decastes and Pleurotus eryngii

Bio-refining of perennial ryegrass (Lolium perenne): evaluation of aqueous extracts for plant defence elicitor activity using French bean cell suspension cultures

Spent Mushroom Substrate Uses

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