Autofluorescence as a tool to study mucus secretion in Eisenia foetida

Heredia, R.B.; Dueñas, S.; Castillo, Luis; Ventura, Javier; Briano, Marcelo Silva; Rio, F. Posadas del; Rodriguez, Martín Gerardo

doi:10.1016/j.cbpa.2007.01.726

Cited by 23 publications

(27 citation statements)

References 21 publications

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“…These vesicles are intact and stable after shedding from the body (Heredia et al, 2008). In the mucus mixture, vesicles from the surface fluids are densely packed.…”

Section: Apparent Viscosity Properties and Yield Stress Of The Mucus mentioning

confidence: 99%

“…Mucus proteins comprise the basic components of innate immunity in earthworms (Kauschke et al, 2007). The epidermal mucus layer forms and maintains an interface with dissolved oxygen for gas exchange; it protects and lubricates the epithelium and other surfaces, providing first-line defense against invading pathogens (Heredia et al, 2008;Wang et al, 2011). Furthermore, epidermal earthworm mucus buffers the pH of the burrow wall (Schrader, 1994).…”

Section: Introductionmentioning

confidence: 99%

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Earthworm epidermal mucus: Rheological behavior reveals drag-reducing characteristics in soil

Zhang

Chen

et al. 2016

Soil and Tillage Research

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“…These vesicles are intact and stable after shedding from the body (Heredia et al, 2008). In the mucus mixture, vesicles from the surface fluids are densely packed.…”

Section: Apparent Viscosity Properties and Yield Stress Of The Mucus mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Earthworm epidermal mucus: Rheological behavior reveals drag-reducing characteristics in soil

Zhang

Chen

et al. 2016

Soil and Tillage Research

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“…It is therefore plausible to hypothesise that expelled eleocytes and/or their granules were responsible for the mucus fluorescence observed by HEREDIA et al (2008) (JOHNSON et al 2012). The amount of riboflavin stored in eleocytes of lumbricid worms is species-specific and changes in a species-specific manner in response to various edaphic factors , but also due to expulsion of coelomocyte-containing coelomic fluid.…”

Section: Riboflavinmentioning

confidence: 99%

Differential Time Course of Restoration of Experimentally Depleted Coelomocytes and Fluorophores in the Earthworm <I>Eisenia andrei</I>

Santocki

Morgan

Płytycz

2016

folia biol (krakow)

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Stressed earthworms expel coelomic fluid containing several vital cellular and soluble components, thus their post-stress recovery has adaptive value. The present manuscript describes the recovery rates of coelomocytes (amoebocytes and eleocytes) and two fluorophores (riboflavin and 4-methylumbelliferyl â-D-glucuronide, MUG) after experimental extrusion by electrostimulation. Analyses were conducted at time points (from 0.5 hour to 7 weeks) by a combination of cell counts, spectrofluorimetric measurements of riboflavin and MUG, and fluorescence microscopy. Coelomic fluid retrieved 30 minutes after extrusion contained <10% of the baseline levels of amoebocytes, eleocytes and riboflavin; the depleted levels of these variables were fully restored after 3, 5, and 7 weeks post-extrusion, respectively. Restored eleocytes were richer in riboflavin than the eleocytes of worms electrostimulated at t 0 . MUG was less severely depleted (to 49% of baseline) than riboflavin, and was restored to the initial level within 1 week post-extrusion. This indicates that MUG, unlike riboflavin, resides mainly within non-coelomocyte cellular location(s); moreover, this fluorophore may be a useful molecular marker for distinguishing even immunologically-compromised E. andrei from closely related composting species.

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“…Riboflavin exists in the cytoplasm of coelomocytes with different contents among earthworm species of various genera [33]. The secreted mucus from intersegmental pores and mouths also produces fluorescence [29,34,35]. Since riboflavin is essential to the regeneration, the stem cells responsible for regeneration accumulate more riboflavin than other cells thus inducing stronger autofluorescence [36].…”

Section: Annelidamentioning

confidence: 99%

Biofluorescence in Terrestrial Animals, with Emphasis on Fireflies: A Review and Field Observation

Jeng¹

2019

Bioluminescence - Analytical Applications and Basic Biology

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The mysterious world of biofluorescence in terrestrial ecosystems is mesmerizing. Though not as ubiquitous as in the ocean, it is not a rare phenomenon on land. Fluorescence occurs in all major phyla of terrestrial animals (Platyhelminthes, Mollusca, Annelida, Nematoda, Onychophora, Arthropoda, and Chordata) and their subgroups, with diverse fluorophores and performance. In this chapter, we make a general review on the fluorescence in terrestrial animals first, including their systematic distribution, research history, fluorophores, and proposed functions for each group among several other aspects. A systematic observation on the fluorescence of fireflies is reported for the first time. The co-occurrence of biofluorescence and bioluminescence in luminescent land snails, earthworms, potworms, millipedes, and fireflies is a fascinating issue. Though the biochemical mechanism of photogenesis is not fully understood in many terrestrial animals except fireflies, it appears that biofluorescence and bioluminescence do not have clear interaction during the light production process. However, fluorophores and luminophores are usually biochemically related and are different from the photogenic mechanism of jellyfish and several marine creatures whose ultimate light emission is made through energy transfer from bioluminescence to biofluorescence by green fluorescent protein (GFP) or its variants. The role of fluorescence is disputative. In general, nocturnal animals or animals having cryptic living styles, e.g., in earth or under shelters like tree bark or rocks, tend to exhibit UV fluorescence more frequently than animals that are diurnal or inhabit open environments. This pattern is evident in fireflies wherein only nocturnal and luminescent species exhibit noticeable UV fluorescence (likely from luciferin), which is dim or absent in diurnal or crepuscular fireflies. It is unlikely that occasionally induced UV fluorescence in natural environments can play a significant role in intra-or interspecific communication in fireflies or other nocturnal animals.

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Autofluorescence as a tool to study mucus secretion in Eisenia foetida

Cited by 23 publications

References 21 publications

Earthworm epidermal mucus: Rheological behavior reveals drag-reducing characteristics in soil

Earthworm epidermal mucus: Rheological behavior reveals drag-reducing characteristics in soil

Differential Time Course of Restoration of Experimentally Depleted Coelomocytes and Fluorophores in the Earthworm <I>Eisenia andrei</I>

Biofluorescence in Terrestrial Animals, with Emphasis on Fireflies: A Review and Field Observation

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