Autofluorescence based diagnostic techniques for oral cancer

Balasubramaniam, A.; Rajkumari, Sunanda; Sindhuja, Pandian; Mohideen, Khadijah; Parameswar, RArjun; Haris, KT Muhamed

doi:10.4103/0975-7406.163456

Cited by 39 publications

(19 citation statements)

References 28 publications

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“…MFs are characterized by actin microfilament bundles (stress fibers) containing smooth muscle α-actin, thus presenting high contractile capacity ( 15 ). MFs are transdifferentiated fibroblasts and the beta growth transforming factor (TGF-β) cytokine plays an important role in directly promoting their development by inducing α-SMA expression ( 10 , 16 - 17 ).…”

Section: Discussionmentioning

confidence: 99%

“…Potential correlations between the angiogenic index and the presence of MF in RC and RRC were also evaluated, only detected in RRC, corroborating another study ( 17 ), which found a positive and moderate correlation between these indices in RRC. In the case of odontogenic lesions, the presence of MF constitutes a source of angiogenic proteins, as well as extracellular matrix degradation proteinases, which together favor lesion growth ( 10 , 15 ).…”

Section: Discussionmentioning

confidence: 99%

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Myofibroblasts and increased angiogenesis contribute to periapical cystic injury containment and repair

de-Freitas

de-França²,

Gordón-Núñez³

et al. 2020

Med Oral

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Background Myofibroblasts (MF) and angiogenesis are important factors in the development and expansion of cystic lesions, where these cells secrete growth factors and proteases, stimulating angiogenesis, matrix deposition and cell migration, affecting the growth of these periapicopathies. The present study aimed to evaluate the immunohistochemical expression of CD34 and α-SMA in radicular cysts (RC) and residual radicular cysts (RRC), with the purpose of contributing to a better understanding of the expansion and progression of these periapical lesions. Material and Methods The present study os a descriptive, quantitative and comparative analysis of positive CD34 and α-SMA immunohistochemical expressions in 30 RC and 30 RRC specimens. α-SMA expression was evaluated in the fibrous capsule of the lesions, at 100x magnification below the epithelial lining. A total of 10 higher immunostaining fields were selected and subsequently, positive cells were quantified at 400x magnification, averaged per field. Regarding the angiogenic index, immuno-labeled microvessel counts for the anti-CD34 antibody were performed in 10 fields at 200x magnification. Results Statistically significant differences regarding α-SMA immunostaining were observed ( p = 0.035), as well as a correlation between α-SMA versus CD34 ( p = 0.004) in RRC. However, the angiogenic index obtained by immunostaining for CD34 indicated no statistical difference between lesions. Intense inflammatory infiltrates were predominant in RC, while mild and moderate degrees were more commonly observed in RRC ( p <0.001). Intense inflammatory infiltrates were also more often noted in larger RRC ( p = 0.041). Inflammatory infiltrates showed no significant correlation with α-SMA and CD34 immunostaining. Conclusions The results indicate that the significant correlation found between the presence of MF and the angiogenic index are related to the repair process in RRC. Key words: Myofibroblasts, angiogenesis, inflammatory odontogenic cysts.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Myofibroblasts and increased angiogenesis contribute to periapical cystic injury containment and repair

de-Freitas

de-França²,

Gordón-Núñez³

et al. 2020

Med Oral

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“…While traditional intraoral cameras with violet or blue light sources employ orange filters, devices designed to assess green fluorescence of soft tissues, such as the VELscope (LED Dental, Atlanta, GA, USA) employ filters to remove the excitation light (in this instance, 400–460 nm) . The results of the present study show the potential value of a selective green filter stack when used with violet or blue light to assess green fluorescence of tooth structure.…”

Section: Discussionmentioning

confidence: 76%

Violet and blue light‐induced green fluorescence emissions from dental caries

Shakibaie

Walsh

2016

Australian Dental Journal

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“…Thus, QD-based nanoprobes can ensure better sensitivity when used in combination with imaging techniques 14 , 29 . This characteristic is essential for the analysis of tumour tissues, which display alterations in the concentration and constitution of extra- and intracellular components, such as collagen, elastin, keratin, haemoglobin, and NADPH, leading in some cases to substantial autofluorescence 30 .…”

Section: Discussionmentioning

confidence: 99%

Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots

Ramos-Gomes

Bode

Sukhanova

et al. 2018

Sci Rep

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Early detection of malignant tumours and, especially, micrometastases and disseminated tumour cells is still a challenge. In order to implement highly sensitive diagnostic tools we demonstrate the use of nanoprobes engineered from nanobodies (single-domain antibodies, sdAbs) and fluorescent quantum dots (QDs) for single- and two-photon detection and imaging of human micrometastases and disseminated tumour cells in ex vivo biological samples of breast and pancreatic metastatic tumour mouse models expressing human epidermal growth factor receptor 2 (HER2) or carcinoembryonic antigen (CEA). By staining thin (5–10 µm) paraffin and thick (50 µm) agarose tissue sections, we detected HER2- and CEA-positive human tumour cells infiltrating the surrounding tissues or metastasizing to different organs, including the brain, testis, lung, liver, and lymph nodes. Compared to conventional fluorescently labelled antibodies the sdAb-HER2-QD and sdAb-CEA-QD nanoprobes are superior in detecting micrometastases in tissue sections by lower photobleaching and higher brightness of fluorescence signals ensuring much better discrimination of positive signals versus background. Very high two-photon absorption cross-sections of QDs and small size of the nanoprobes ensure efficient imaging of thick tissue sections unattainable with conventional fluorescent probes. The nanobody–QD probes will help to improve early cancer diagnosis and prognosis of progression by assessing metastasis.

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Autofluorescence based diagnostic techniques for oral cancer

Cited by 39 publications

References 28 publications

Myofibroblasts and increased angiogenesis contribute to periapical cystic injury containment and repair

Myofibroblasts and increased angiogenesis contribute to periapical cystic injury containment and repair

Violet and blue light‐induced green fluorescence emissions from dental caries

Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots

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