Violet and blue light‐induced green fluorescence emissions from dental caries

Shakibaie, Fardad; Walsh, L. J.

doi:10.1111/adj.12414

Cited by 18 publications

(14 citation statements)

References 17 publications

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“…"Black-pigmented" organisms give strong visible red emissions when exposed to ultraviolet or visible violet light because of their high porphyrin content. The same effects occurs in mature dental plaque biofilms on the surfaces of teeth and in dental caries [22][23][24]. There are also green emissions from the collagen component of dentine.…”

Section: Fluorescence Using Ultraviolet Light or Visible Violet Lightmentioning

confidence: 79%

Novel Approaches to Detect and Treat Biofilms within the Root Canals of Teeth: A Review

Walsh

2020

Antibiotics

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Biofilms located within the root canals of teeth are a unique and pressing concern in dentistry and in medical microbiology. These multispecies biofilms, which include fungi as well as bacteria, form in a protected site with low shear stress and low oxygen tension. Systemic antibiotics are of limited value because of the lack of blood flow of the site, and issues with innate and acquired resistance. Physical disruption using hand or rotary powered instruments does not reach all locations in the root canal system where biofilms are present. Alternative strategies including agitated irrigation fluids, continuous chelation, materials with highly alkaline pH, and antimicrobial nanoparticles are being explored to meet the challenge. Detection and quantification of biofilms using fluorescence-based optical methods could provide an indication of successful biofilm removal and an endpoint for physical and chemical treatments.

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Section: Fluorescence Using Ultraviolet Light or Visible Violet Lightmentioning

confidence: 79%

Novel Approaches to Detect and Treat Biofilms within the Root Canals of Teeth: A Review

Walsh

2020

Antibiotics

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“…In the past studies, we have shown that there is a strong correlation with the volume of calculus deposits [1]. How much dental plaque [22][23][24] and other substances [25] that may be present under clinical conditions adversely influence the performance of LF and other optical systems must be assessed through controlled clinical trials.…”

Section: Discussionmentioning

confidence: 99%

Improved detection of subgingival calculus by laser fluorescence over differential reflectometry

2019

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Laser fluorescence (LF) and differential reflectometry (DR) are two new optical methods which have been used to help diagnose subgingival deposits of dental calculus. This study compared the performance of LF using the KEY3 laser system versus DR using the DetecTar system under controlled laboratory conditions designed to simulate clinical conditions as much as possible. A total of 30 extracted human posterior teeth were set in an anatomical configuration in stone typodonts with impression material replicating the periodontal soft tissues. LF was more accurate than DR (76.2% vs. 68.2%) and gave higher reproducibility (Bangdiwala's B statistic 0.71 vs. 0.54). The better performance of LF makes it the preferred option of the two methods for detection of subgingival dental calculus.

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“…At 400-500 nm excitation wavelengths, the major and minor fluorescent peaks are mostly within the range of 610-614 nm, whereas with excitation at wavelengths above 500 nm, the major and minor peaks are now mainly in the near-infrared spectrum, particularly around 825 nm. The fluorescence profiles for dental plaque or biofilms on the tooth surfaces are less intense in the visible red spectrum than those for dental caries and dental calculus [53]. Zn-protoporphyrin 13 8…”

Section: Porphyrins and Biofilm Fluorescencementioning

confidence: 99%

“…This selective fluorophore approach has been used for kidney stones, tumours, dental filling materials [39,40], dental caries [41], dental plaque biofilms [2] and dental calculus [42]. The latter four sample types have been identified in diseased and healthy sites, employing optimal excitation wavelengths for fluorescence detection and then coupling this to a feedback-controlled second laser system for ablation.…”

Section: Bacterial Biofilmsmentioning

confidence: 99%

“…CCD image sensors are considered to have better sensitivity for light detection than CMOS but are more expensive. They can operate well for detecting light emissions in the near infrared, which is useful for detection of bacteria [41,52,53], provided that a long-pass filter is used to remove reflected excitation light as well as ambient daylight and work-place lighting. In the case of the DIAGNOdent, only light wavelengths above 680 nm are measured [45][46][47].…”

Section: Bacterial Biofilmsmentioning

confidence: 99%

See 1 more Smart Citation

Application of Fluorescence Spectroscopy for Microbial Detection to Enhance Clinical Investigations

Shakibaie¹,

Lamard²,

Rubinsztein‐Dunlop³

et al. 2018

Photon Counting - Fundamentals and Applications

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Microbial biofilms are complex multi-layered communities of bacteria and fungi which cause a range of oral and other diseases. Efficient detection of biofilms is important for the clinical management of diseases they cause and for providing an endpoint to clinical treatments. For bacterial biofilms, bacterial metabolites such as porphyrins are important molecules for diagnostic purposes, since they fluoresce in the red and infrared regions of the spectrum. Fluorescence is a versatile and powerful diagnostic approach for detection of bacterial biofilms, particularly in dentistry. This chapter provides an overview of fluorescence spectroscopic methods for detection and analysis of biofilms and their derivatives such as deposits of dental calculus and how current technology can be extended using photon-counting detectors. Fluorescence can be used to help discriminate these from healthy tissues. The approaches described have broad applications to clinical and industrial situations where non-invasive detection of microbial biofilms is important.

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Violet and blue light‐induced green fluorescence emissions from dental caries

Abstract: Using violet or blue illumination in combination with green compensating filters could potentially aid in the assessment of areas of mineral loss.

Cited by 18 publications

References 17 publications

Novel Approaches to Detect and Treat Biofilms within the Root Canals of Teeth: A Review

Novel Approaches to Detect and Treat Biofilms within the Root Canals of Teeth: A Review

Improved detection of subgingival calculus by laser fluorescence over differential reflectometry

Application of Fluorescence Spectroscopy for Microbial Detection to Enhance Clinical Investigations

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