Autofluorescence imaging of pingueculae

Utine, Canan Aslı; Tatlıpınar, Sinan; Altunsoy, Muhsin; Oral, Deniz; Başar, Demir; Alimgil, Levent

doi:10.1136/bjo.2008.144055

Cited by 8 publications

(24 citation statements)

References 19 publications

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“…The presence of fluorescence (in at least one region) was 0% in 27 children aged 3 to 8 years, 6 of 23 (26%) for those ages 9-11 years and 17 of 21 (81%) for those ages 12 to 15 years. Using a confocal scanning laser ophthalmoscope, Utine et al 30 measured AF in 40 eyes in 23 patients with pinguceulae. In all of the cases, the size of AF in the pinguecula was greater than the extent of the visible pinguecula on slit-lamp examination in 71.4% of the lesions.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, they found that a Vogt limbal girdle displayed hyperautofluorescence, but arcus senillis did not. 30 AF of the fundus has been used in the imaging of several posterior segment diseases to detect changes in lipofuscin in the retinal pigment epithelium. Such disorders have included rare diseases such as central areolar choroidal dystrophy, 31 Stargardt dystrophy and fundus flavimaculatus, 32 Kjellin's syndrome, 33 multifocal choroiditis and panuveitis, 34 Best's vitelliform dystrophy 35 and birdshot chorioretinopathy.…”

Section: Discussionmentioning

confidence: 99%

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Distribution of conjunctival ultraviolet autoflourescence in a population-based study: the Norfolk Island Eye Study

Sherwin

Hewitt

Kearns

et al. 2011

Eye

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Objective The objective of this study was to describe the distribution of conjunctival ultraviolet autofluorescence (UVAF) in an adult population. Methods We conducted a cross-sectional, population-based study in the genetic isolate of Norfolk Island, South Pacific Ocean. In all, 641 people, aged 15 to 89 years, were recruited. UVAF and standard (control) photographs were taken of the nasal and temporal interpalpebral regions bilaterally. Differences between the groups for non-normally distributed continuous variables were assessed using the Wilcoxon-Mann-Whitney ranksum test. Trends across categories were assessed using Cuzick's non-parametric test for trend or Kendall's rank correlation s. Results Conjunctival UVAF is a nonparametric trait with a positively skewed distribution. Median amount of conjunctival UVAF per person (sum of four measurements; right nasal/temporal and left nasal/temporal) was 28.2 mm 2 (interquartile range 14.5-48.2). There was an inverse, linear relationship between UVAF and advancing age (Po0.001). Males had a higher sum of UVAF compared with females (34.4 mm 2 vs 23.2 mm 2 , Po0.0001). There were no statistically significant differences in area of UVAF between right and left eyes or between nasal and temporal regions. Conclusion We have provided the first quantifiable estimates of conjunctival UVAF in an adult population. Further data are required to provide information about the natural history of UVAF and to characterise other potential disease associations with UVAF. UVR protective strategies should be emphasised at an early age to prevent the long-term adverse effects on health associated with excess UVR.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Distribution of conjunctival ultraviolet autoflourescence in a population-based study: the Norfolk Island Eye Study

Sherwin

Hewitt

Kearns

et al. 2011

Eye

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“…This is the first study, to our knowledge, to measure CUVAF area using blue laser autofluorescence on a cSLO. Utine and colleagues described a similar method of detecting autofluorescence within pingueculae using the same cSLO; however, they did not quantify CUVAF area in their study . The ability to use a commercially available cSLO, that is already present in many ophthalmic clinics to detect and measure CUVAF area, allows CUVAF imaging to be more easily adopted by clinicians and researchers.…”

Section: Discussionmentioning

confidence: 99%

Repurposing blue laser autofluorescence to measure ocular sun exposure

Lingham

Burton

Brown

et al. 2018

Clinical Exper Ophthalmology

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Importance Excessive ocular sun exposure is linked to various eye pathologies. Conjunctival ultraviolet autofluorescence (CUVAF) is a method of detecting sun‐related conjunctival damage; however, the custom‐built camera system required is not readily available. Background We investigated whether blue laser autofluorescence (BAF) on a commonly used confocal scanning laser ophthalmoscope (cSLO) can be utilized to measure CUVAF area. Design Cross‐sectional evaluation of a diagnostic technology at a medical research institute. Participants Sixty‐four participants recruited from three on‐going observational eye studies in Western Australia. Methods All participants had four images, two of each eye, captured using the CUVAF camera and BAF on the same day. Participants with pterygium or poor quality images were excluded from the analysis. Two graders measured CUVAF area in each image twice. CUVAF area measured by BAF was then compared to measurements determined with the conventional camera system. Main Outcome Measures CUVAF area. Results After exclusions, 50 participants' images were analysed. Intra‐ and inter‐observer repeatability were similar between the two systems. When comparing CUVAF area measured by BAF to the camera measurement, grader 1 had a mean difference of +1.00 mm2, with 95% limits of agreement −5.75 to 7.77 mm2. Grader 2 had a mean difference of +0.21mm2, with 95% limits of agreement −7.22 to 7.64 mm2. Conclusions and Relevance BAF on a commercially available cSLO is a valid method for measuring CUVAF area. This finding provides broader opportunity for identifying, monitoring and educating patients with sun‐exposure‐related ocular conditions and for researching the ocular impacts of sun exposure.

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“…Recently, Utine et al [9] demonstrated that hyper-AF originates from pingueculae using confocal scanning laser ophthalmoscopy. They compared the anterior images to hyper-fluorescence areas.…”

Section: Discussionmentioning

confidence: 99%

“…Utine et al [9] reported that pingueculae display hyper-AF on confocal scanning laser ophthalmoscopy. The actual pinguecula size can be estimated by AF characteristics, and is generally larger than the visible lesion size.…”

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confidence: 99%

The Pathologic Characteristics of Pingueculae on Autofluorescence Images

Kim

Chun

Kim

2013

Korean J Ophthalmol

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PurposeTo analyze the autofluorescence (AF) properties of pinguecula using cobalt-blue and yellow filters and to investigate the nature and pathogenesis of pingueculae using histochemical and immunohistochemical staining.MethodsFifty pingueculae in 40 patients were included in this study. AF of the pingueculae was observed and analyzed using a cobalt-blue filter with an additional yellow filter on a slit-lamp. Hematoxylin-eosin and immunohistochemical stainings were performed on surgical specimens of pingueculae that were prepared from each patient. Immunohistochemical staining included Congo red, Oil Red O, periodic acid-Schiff (PAS), Masson's trichrome, transglutaminase-2 (TG-2), mesenchymal stem cell markers CD29 (β-1-integrin), and CD34.ResultsAF images revealed hyper-AF in the pinguecula area. The AF lesions of pingueculae showed superficial punctuate erosions and avascular lesions. Deposition of eosinophilic and amorphous materials in the subepithelial layer of the pinguecula were observed on hematoxylin-eosin staining. Historeactivities to Congo red, PAS, Oil Red O, alcian blue, and Masson's trichrome were not detected, but immunoreactivities to CD29, CD34, and TG-2 were detected in the pingueculae with AF. However, CD29, CD34, and TG-2 were not detected in the pingueculae without AF.ConclusionsThe AF of pingueculae may be related to CD29, CD34, and TG-2. We suggest that pingueculae with AF have a different pathogenesis compared to pingueculae without AF.

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Autofluorescence imaging of pingueculae

Abstract: Pingueculae display hyperautofluorescence in AF imaging. The real size of pingueculae may be estimated by its AF characteristics, which is mostly larger than the visible lesion.

Cited by 8 publications

References 19 publications

Distribution of conjunctival ultraviolet autoflourescence in a population-based study: the Norfolk Island Eye Study

Distribution of conjunctival ultraviolet autoflourescence in a population-based study: the Norfolk Island Eye Study

Repurposing blue laser autofluorescence to measure ocular sun exposure

The Pathologic Characteristics of Pingueculae on Autofluorescence Images

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