Autoimmune Neutropenia in Infancy Due to Anti-NA1 Antibody: Detection of Antibody with Immunofluorescence and Agglutination Test

Significance of the detection of antineutrophil antibodies in children with chronic neutropenia

“…8 Monoclonal antibodies, TAG1, TAG2, and TAG4 that recognize neutrophilspecific antigens, HNA-1 and HNA-2, were originally established. The characteristics of TAG1 and TAG2 were identified by comparing the binding properties of MG38 (Nichirei, Tokyo, Japan), GRM1 (a kind gift from Dr Garrido, Granada University, Granada, Spain), and 3G8 (Pharmingen, San Diego, CA).…”

“…23,24 GIIFT and micro-leukocyte agglutination test GIIFT and micro-leukocyte agglutination test (MLAT) were performed by using a modification of the previously described method. 8 In GIIFT, fluorescence isothiocyanate-labeled F(abЈ) 2 fragments of goat antihuman polyvalent immunoglobulin, immunoglobulin (Ig)G, IgM, and IgA (Tago, Burlingame, CA) were used as the second antibody. The cell suspension was examined by using flow cytometry (FACS Vantage; Becton Dickinson Immunocytometry Systems, San Jose, CA) with 4-W argon ion laser and/or ORTHO CYTRON ABSOLUTE (Ortho-Clinical Diagnostics K.K., Tokyo, Japan) by using a 15-mW argon ion laser.…”

“…Chronic benign neutropenia (CBN) and primary autoimmune neutropenia (AIN) are common forms of neutropenia in infants and early childhood. [1][2][3][4][5][6][7][8][9][10][11] Both types of neutropenia have shown similar hematologic findings along with their clinical features. The definitive distinction is the presence or absence of circulating antineutrophil antibodies in serum.…”

“…3,4,7 Several methods are used in the detection of serum antineutrophil antibodies. [5][6][7][8][12][13][14][15][16][17] However, the specificity and sensitivity of these methods have not been well established. In this study, we have developed a method to semiquantify the strength of antineutrophil antibodies by flow cytometric analysis of the granulocyte indirect immunofluorescence test (GIIFT) by using same donor neutrophils.…”

Identification of a nonsense mutation in the granulocyte-colony-stimulating factor receptor in severe congenital neutropenia.

“…Patients also have an increased risk for developing acute leukemia (2). Although an autosomal recessive pattern of inheritance was suggested for SCN, sporadic cases have been reported as well (3)(4)(5)(6).…”