Autologous dendritic cells pulsed with allogeneic tumour cell lysate induce tumour-reactive T-cell responses in patients with pancreatic cancer: A phase I study

Dataset from a proteomics analysis of tumor antigens shared between an allogenic tumor cell lysate vaccine and pancreatic tumor tissue.

“…The study was approved by the Central Committee on Research involving Human Subjects (NL67169.000.18) and enrolled in the Dutch trial register (registration number NL7432, https://www.trialregister.nl ). Further description about the study, patient inclusion criteria and sample collection were described in detail by Lau and co-workers [1] . In brief, PDAC tissue samples were obtained during surgery (pancreaticoduodenectomy or distal pancreatectomy and splenectomy) and patients were included in retrospect into the study when the diagnosis was confirmed by pathological review and fresh-frozen tumor material was available.…”

“…In brief, PDAC tissue samples were obtained during surgery (pancreaticoduodenectomy or distal pancreatectomy and splenectomy) and patients were included in retrospect into the study when the diagnosis was confirmed by pathological review and fresh-frozen tumor material was available. Tumor lysates were prepared by manual cutting of the tissue samples into smaller fragments and bead-mediated homogenization in 1 mL Milli-Q for four cycles of 3 min [1] . A Bradford assay was performed to quantify the protein concentration of the tumor lysates before they were aliquoted and stored below -70°C until further analysis.…”

“… The dataset may serve as basis (trainings set) for in-silico studies of modelling and predicting peptide properties and behaviors. The dataset provides information about peptides of tumor antigen candidates [1 , 2] that were quantified with a data-dependent shotgun method using pre-defined inclusions list of peptide precursors and peptide-fragments for SPS-MS3 TMT quantification. The data can be used for further development of analysis and acquisition software to analyze SPS-MS3 spectra and FAIMS data.…”

“…The dataset provides information about peptides of tumor antigen candidates [1 , 2] that were quantified with a data-dependent shotgun method using pre-defined inclusions list of peptide precursors and peptide-fragments for SPS-MS3 TMT quantification.…”

“…We describe two datasets that were acquired in order to detect and quantify shared tumor antigens between an allogeneic tumor cell line lysate (PheraLys) and tissue samples from pancreatic ductal adenocarcinoma (PDAC) of 9 patients as described by Lau and colleagues [1] . Prior to the LC-MS measurements, all samples were enzymatically digested with trypsin, TMT -labelled, pooled in six-plex, and fractionated into 24 fractions with high-pH reversed phase preparative chromatography.…”