Autologous endometrial coculture biopsy: is timing everything?

Melnick, A.P.; Murphy, Erin M.; Masbou, A.K.; Sapra, Katherine J.; Rosenwaks, Zev; Spandorfer, Steven D.

doi:10.1016/j.fertnstert.2015.04.026

Cited by 4 publications

(4 citation statements)

References 22 publications

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“…A study of oocyte donation recipients demonstrated no increase in implantation, clinical pregnancy or live birth rates in patients who underwent a single endometrial injury by a pipelle catheter performed in luteal phase compared as compared with controls. [ 7 ].A more recent study of patients with a history of at least one IVF failure undergoing a single luteal phase endometrial biopsy by a Pipelle catheter, demonstrated that biopsy in the cycle preceding IVF did not increase implantation, clinical pregnancy, or live birth rates compared with biopsy performed more than one cycle before IVF [ 18 ]. Recently, a systematic literature review about endometrial injury for RIF concluded that evidence is lacking for endometrial injury to be used in women with RIF undergoing ART [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…Many studies reported on outcomes of endometrial injury in women with recurrent implantation failure (RIF) with conflicting results. While some studies demonstrated that implantation rates, and clinical pregnancy rates improved after endometrial injury [ 9 , 10 , 13 – 17 ], others showed no benefit [ 5 , 7 , 18 , 19 ]. Nevertheless, studies on using endometrial injury in unselected women undergoing IVF are scarce [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of local endometrial injury in proliferative vs. luteal phase on IVF outcomes in unselected subfertile women undergoing in vitro fertilization

Liu

Tal

et al. 2017

Reprod Biol Endocrinol

124

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BackgroundMechanical endometrial injury prior to IVF has been suggested as a means to increase implantation rates by improving endometrial receptivity. However, the effects of endometrial injury in proliferative vs. luteal phase have not been studied before. This study aimed to explore whether endometrial injury in the proliferative phase of the preceding cycle before in vitro fertilization/embryo transfer (IVF-ET) improves the clinical outcomes in unselected subfertile women compared with injury in luteal phase.MethodsA group of 142 patients who were good responders to hormonal stimulation were randomized into four groups: injury group (group A: endometrial injury in proliferative phase, n = 38; group B: endometrium injury in luteal phase, n = 32), and non-injury group as control (group C: non-injury in proliferative phase, n = 36; group D: non-injury in luteal phase, n = 36). Patients in injury groups underwent endometrial injury in either proliferative phase or luteal phase in the preceding cycle before IVF treatment. Clinical outcomes including implantation, pregnancy, and live birth rates were analyzed among the four groups.ResultsThe baseline characteristics of the four groups including age, body mass index, duration, type and causes of infertility were similar. There were no significant differences in implantation, clinical pregnancy or live birth rates between injury group and non-injury group. Moreover, there were also no significant differences in implantation, clinical pregnancy, or live birth rates in injury in proliferative phase compared with luteal phase.ConclusionsEndometrial injury in the cycle preceding IVF of unselected subfertile women does not increase implantation, clinical pregnancy, or live birth rates. Furthermore, there is no significant difference in clinical outcomes between endometrial injury in the proliferative phase and injury in the luteal phase.Trial registrationThis study was retrospectively registered on May 26th, 2017 (ChiCTR-IOR-17011506).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of local endometrial injury in proliferative vs. luteal phase on IVF outcomes in unselected subfertile women undergoing in vitro fertilization

Liu

Tal

et al. 2017

Reprod Biol Endocrinol

124

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“…However, the studies came to inconsistent conclusions. Several studies show an enhanced implantation rate after scratching [ 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 ], while there are also several studies that could not or barely show significant differences due to scratching [ 139 , 140 , 141 , 142 , 143 , 144 , 145 , 146 , 147 , 148 , 149 , 150 , 151 , 152 , 153 , 154 , 155 , 156 , 157 , 158 , 159 ...…”

Section: Clinical Significance In Reproductive Medicinementioning

confidence: 99%

Immune Cell Functionality during Decidualization and Potential Clinical Application

Stope

Mustea

Sänger

et al. 2023

Life

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Due to a vast influx in the secretory phase of the menstrual cycle, leukocytes represent 40–50% of the decidua at the time of implantation. Their importance for the implantation, maintenance of pregnancy, and parturition are known yet not fully understood. Thus, in idiopathic infertility, decidual immune-related factors are speculated to be the cause. In this review, the immune cell functions in the decidua were summarized, and clinical diagnostics, as well as interventions, were discussed. There is a rising number of commercially available diagnostic tools. However, the intervention options are still limited and/or poorly studied. In order for us to make big steps towards the proper use of reproductive immunology findings, we need to understand the mechanisms and especially support translational research.

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“…However, the rate of live births per embryo transfer through assisted reproductive technologies remains as low as 30%, so there is still a substantial need to improve current human IVF-ET procedures by determining optimal embryonic culture conditions [3]. For patients with repeated IVF failures, the methods to improve IVF outcomes include focusing on improvement in embryo quality and implantation rates, because repeated IVF failure occurs due to unsuccessful implantation, according to the generally accepted view [4].…”

Section: Introductionmentioning

confidence: 99%

Is leptin receptor expression triggered in case of embryo transfer to endometrium co-culture?

Kaplanoğlu¹,

Cınar

et al. 2019

Turk J Med Sci

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Background/aim: A synchronized dialogue between maternal and embryonic tissues is required for a successful implantation. Low uterine receptivity is responsible for two-thirds of implantation failures and leptin is effective in physiology of reproduction by binding to specific receptors. In this study, we investigate leptin receptor expression in cases of embryo transfer to endometrial co - culture. Material and methods: Biopsy materials were taken from 20 females with indication for co - culture application and were cultured in appropriate medium, after the epithelial cells were isolated. The grown cells were cultured in chamber slides as the first group. For the second group, day 3 embryo was added to chamber slides and the development was observed. 1 - 2 days later, the embryo was transferred and other cells (after the transfer process) were used to form the second group. After fixation, immunohistochemical staining with anti-leptin primary antibody was done. Results: Regarding the co-culture without the embryo transfer, moderate leptin receptor immunoreactivity was seen in perinuclear region and in cell membrane. And regarding co-culture with the embryo transfer, moderate leptin receptor immunreactivity was seen in cytoplasm and strong leptin receptor immunoreactivity was seen in cell membrane. Conclusion: Embryo transfer to the endometrium co-culture triggers leptin receptor expression.

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Autologous endometrial coculture biopsy: is timing everything?

Cited by 4 publications

References 22 publications

Effect of local endometrial injury in proliferative vs. luteal phase on IVF outcomes in unselected subfertile women undergoing in vitro fertilization

Effect of local endometrial injury in proliferative vs. luteal phase on IVF outcomes in unselected subfertile women undergoing in vitro fertilization

Immune Cell Functionality during Decidualization and Potential Clinical Application

Is leptin receptor expression triggered in case of embryo transfer to endometrium co-culture?

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