Autologous full-thickness skin substitute for healing chronic wounds

Gibbs, Susan; Hoogenband, Henk M. van den; Kirtschig, Gudula; Richters, C. D.; Spiekstra, Sander W.; Breetveld, Melanie; Scheper, Rik J.; Boer, E. M. de

doi:10.1111/j.1365-2133.2006.07266.x

Cited by 78 publications

(72 citation statements)

References 29 publications

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“…ASC may be more suited for use in skin constructs designed for chronic wounds in which activation of the inert wound bed is essential for healing. 30 This is in line with others who showed that ulcers treated with ASC in rats showed increased capillary density and granulation tissue thickness. 46 In contrast, dermal fibroblasts may be more suited for use in skin constructs designed for the treatment of deep burns where overactivation of the wound bed and stimulation of excessive granulation tissue needs to be avoided in order to reduce the chance of hypertrophic scar formation.…”

supporting

confidence: 90%

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Differential Response of Human Adipose Tissue-Derived Mesenchymal Stem Cells, Dermal Fibroblasts, and Keratinocytes to Burn Wound Exudates: Potential Role of Skin-Specific Chemokine CCL27

Broek

Kroeze

Waaijman

et al. 2014

Tissue Engineering Part A

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Many cell-based regenerative medicine strategies toward tissue-engineered constructs are currently being explored. Cell-cell interactions and interactions with different biomaterials are extensively investigated, whereas very few studies address how cultured cells will interact with soluble wound-healing mediators that are present within the wound bed after transplantation. The aim of this study was to determine how adipose tissue-derived mesenchymal stem cells (ASC), dermal fibroblasts, and keratinocytes will react when they come in contact with the deep cutaneous burn wound bed. Burn wound exudates isolated from deep burn wounds were found to contain many cytokines, including chemokines and growth factors related to inflammation and wound healing. Seventeen mediators were identified by ELISA (concentration range 0.0006-9 ng/mg total protein), including the skin-specific chemokine CCL27. Burn wound exudates activated both ASC and dermal fibroblasts, but not keratinocytes, to increase secretion of CXCL1, CXCL8, CCL2, and CCL20. Notably, ASC but not fibroblasts or keratinocytes showed significant increased secretion of vascular endothelial growth factor (5-fold) and interleukin-6 (253-fold), although when the cells were incorporated in bi-layered skin substitute (SS) these differences were less pronounced. A similar discrepancy between ASC and dermal fibroblast mono-cultures was observed when recombinant human-CCL27 was used instead of burn wound exudates. Although CCL27 did not stimulate the secretion of any of the wound-healing mediators by keratinocytes, these cells, in contrast to ASC or dermal fibroblasts, showed increased proliferation and migration. Taken together, these results indicate that on transplantation, keratinocytes are primarily activated to promote wound closure. In contrast, dermal fibroblasts and, in particular, ASC respond vigorously to factors present in the wound bed, leading to increased secretion of angiogenesis/granulation tissue formation factors. Our findings have implications for the choice of cell type (ASC or dermal fibroblast) to be used in regenerative medicine strategies and indicate the importance of taking into account interactions with the wound bed when developing advanced therapies for difficult-to-close cutaneous wounds.

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confidence: 90%

“…30 Keratinocytes (5 · 10 5 cells/SS) were seeded on top of acellular human dermis (prepared from glycerol-preserved donor skin [Euro Skin Bank, Beverwijk, The Netherlands]) and cultured, submerged, in keratinocyte medium. ASC and dermal fibroblasts were cultured in 0.4 mm pore size transwells (Cat.…”

Section: Cell Isolation and Culturementioning

confidence: 99%

Differential Response of Human Adipose Tissue-Derived Mesenchymal Stem Cells, Dermal Fibroblasts, and Keratinocytes to Burn Wound Exudates: Potential Role of Skin-Specific Chemokine CCL27

Broek

Kroeze

Waaijman

et al. 2014

Tissue Engineering Part A

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“…Using fibroblasts to treat chronic and acute ulcers relieves the pain, accelerates the healing process with fewer scars, and from another viewpoint, improves cosmetic results (13). After application of cutaneous equivalents on surgical acute ulcers, their effect on the stimulating granular tissues was confirmed and the transplanted tissue was not rejected (14). The results of the current study recommended the application of fibroblasts as an effective therapeutic modality to treat recalcitrant DFUs.…”

Section: Discussionmentioning

confidence: 59%

Treatment of Recalcitrant Diabetic Ulcers With Trichloroacetic Acid and Fibroblasts

et al. 2014

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Introduction: Foot ulcer, with a prevalence of 15% in general population, is one of the main complications in patients with diabetes. So far, different therapeutic methods have been provided for the treatment of foot ulcers. When no response to the standard treatment methods is observed, other methods would be applied. Case Presentation: In this report, we introduce two men with type 2 diabetes mellitus and recalcitrant diabetic foot ulcer who were treated with topical 50% trichloroacetic acid solvent and autologous fibroblasts. The treatment resulted in complete healing of foot ulcers in both patients. Conclusions: Trichloroacetic acid and autologous fibroblast can be considered as therapeutic choice for recalcitrant diabetic foot ulcer.

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“…One milliliter of the culture medium was poured into each well of the CES including cellular suspension and the CES without cellular suspension as a control were put on. The CESs were incubated at 37°C in 5% of CO 2 , with fresh medium changes evon tissue engineering and the clinical applications have already been reported as the most advanced therapy (11,27,28). The development of the scaffold which is mainly made from collagen may promote the expansion of tissue engineering methods and may make a contribution to perform clinical application in regenerative medicine near future.…”

Section: -D Culture Of Fibroblasts In Cesmentioning

confidence: 99%

The usefulness of the collagen and elastin sponge derived from salmon as an artificial dermis and scaffold for tissue engineerin

Matsumoto

Ikeda

Yamaya³

et al. 2011

Biomed. Res.

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Collagen sponge is one of the medical materials that are frequently used in clinical medicine. However, the problem of prion disease harmfully affected the usage of mammals-derived medical materials. Since there have been no reports about prion disease occurring in marine products, we produced the collagen and elastin sponge (CES) made from salmon, and investigated whether the CES could be a substitute for mammalian collagen sponge. Fibroblasts were seeded in the CES to examine whether the CES could be used as a scaffold for tissue engineering. The results of the WST-1 assay showed that the fibroblasts were viable and were well proliferated in the CES. To examine whether the CES could be used as an artificial dermis, the CES and TERUDERMIS (traditional collagen sponge) were grafted onto the skin defects on the dorsum of rats. The histological findings of these ulcers showed non-significant difference between the CES and TERUDERMIS. Because of the safety, the abundance of the resources, and the possessing same ability as TERUDERMIS, the biomedical materials derived from marine products may be a substitute for those derived from mammals.Collagen sponge is one of the biomedical materials frequently used in clinical medicine, especially in dermatology and plastic reconstructive surgery. It has been used as a biomaterial which covers fullthickness skin defects for the treatments of diseases and conditions such as burn (13,30,42), trauma (16), chronic skin ulcer (2, 10), excised skin tumor (23,33,38) and others that cause full-thickness skin defects. The main ability of collagen sponge is to accelerate tissue granulation (7), as forming good granulation from a relatively early stage-even on a tendon (18, 37) and bone (16,23,38,42), which generally takes a long time to form. And this ability is able to make a wound heal fast by using secondary skin grafting. The combined treatment with collagen sponge and skin grafting is less invasive for patients than other operative methods covering skin defects, such as local skin flaps, pedicled flaps, and free flaps because it is technically easy and takes a short time to perform the combination treatment (23,38). Collagen sponge is a very important treatment tool in dermatology and plastic reconstructive surgery. Collagen sponge is expected to be used as a scaffold for tissue engineering because of its high ability of cell adherence and biological affinity. It has contributed to regenerating various tissues such as

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Autologous full-thickness skin substitute for healing chronic wounds

Abstract: Application of this novel skin substitute provides a promising new therapy for healing chronic wounds resistant to conventional therapies.

Cited by 78 publications

References 29 publications

Differential Response of Human Adipose Tissue-Derived Mesenchymal Stem Cells, Dermal Fibroblasts, and Keratinocytes to Burn Wound Exudates: Potential Role of Skin-Specific Chemokine CCL27

Differential Response of Human Adipose Tissue-Derived Mesenchymal Stem Cells, Dermal Fibroblasts, and Keratinocytes to Burn Wound Exudates: Potential Role of Skin-Specific Chemokine CCL27

Treatment of Recalcitrant Diabetic Ulcers With Trichloroacetic Acid and Fibroblasts

The usefulness of the collagen and elastin sponge derived from salmon as an artificial dermis and scaffold for tissue engineerin

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