Autologous micrografts from the palatal mucosa for bone regeneration in calvarial defects in rats: a radiological and histological analysis

Abstract

Background The application of dental implants is often restricted by bone volume. In such cases, bone grafts are required, although bone graft materials have some disadvantages. Therefore, other effective approaches are needed. Our previous study showed that the autologous micrograft, a dissociated cell suspension made out of palatal connective tissue grafts, promoted bone-marrow cell proliferation and differentiation under osteogenic conditions. In this study, we aimed to evaluate the effects of dissociated soft-tissue suspensions relevant to bone regeneration in animal model. Material and methods Twelve-week-old male Wistar rats were used in the study. Defects were created in rat calvaria, and were filled with hydrogel containing either dissociated soft-tissue suspension (test) or sucrose (control). The new bone formation was evaluated at 1 and 2 weeks after surgery (n = 16) by radiological and histological analysis. Results The conducted radiological analysis showed that the new bone volume was significantly greater in the dissociated soft-tissue suspension group. This finding was further confirmed by the conducted histological analysis. Conclusions The dissociated mucosa tissue suspension enhanced bone regeneration in vivo; thus, it is a promising potential method to aid the successful application for bone augmentation in the implant practice.

Long-term results of autologous periosteum-derived micro-grafts with poly(lactic-go-glycolic acid) in sinus lift augmentation surgeries: A 7-years follow-up observational study

“…Data from this study cover the same sample for which a six-month follow-up was published. Previously in-vitro and in-vivo studies on a murine model have demonstrated the effectiveness of the AMGs [18] , [19] . Formerly a case report with a follow-up of three years had been published and demonstrated in a single case the effectiveness of this method in the medium term.…”

A Comparative Study of HA/DBM Compounds Derived from Bovine and Porcine for Bone Regeneration

“…Finally, the inflammation was quantified by measuring the ratio between the area of blood vessels (mm 2 ) and the total regenerated area (mm 2 ). This last measurement included hard and soft tissue within the location of the defect [ 44 ]. SI-5 contains additional information and specific methods for calculating these variables.…”

Diagnosis and Orthodontic Treatment of Obstructive Sleep Apnea Syndrome Children—A Systematic Review

“…Prevalence values range from 14% to 78% [67]. These differences result from how obesity and OSAS are defined by different authors [68][69][70][71][72][73]. Studies have shown that the degree of obesity and the severity of OSAS are correlated and that adenotonsillary hypertrophy is more common as a risk factor in obese subjects than in the non-obese population with OSAS [18,74,75].…”