Autologous peripheral hematopoietic stem cell transplantation restores hematopoietic function following marrow ablative therapy

Kessinger, Anne; Armitage, JO; Landmark, James D.; Smith, Douglas M.; Weisenburger, Dennis D.

doi:10.1182/blood.v71.3.723.723

Cited by 360 publications

(33 citation statements)

References 16 publications

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“…A sample was taken from products of leukapheresis at the end of the procedure, generally on alternate collection days, and assayed for CFU-GM colonies (n = 56) and the number of CD34+ cells (n = 69). A previously published agar system was used for the CFU-GM assay [5], with some modification. We added 3,000 U IL-3 instead of normal peripheral blood MNC as a source of colonystimulating factors.…”

Section: Progenitor Cell Assaysmentioning

confidence: 99%

Effects of whole blood flow rates on mononuclear cell yields during peripheral blood stem cell collection using fenwal CS 3000 plus

Lin

Burgstaler

Pineda

et al. 1995

J of Clinical Apheresis

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It is generally assumed that using high whole blood flow rates (WBFR), 80 ml/min, during peripheral blood stem cell (PBSC) collection on the Fenwal CS 3000 Plus blood cell processor will result in higher yields of mononuclear cells (MNC) than using lower WBFR (50 ml/min). To test this assumption, we retrospectively studied 129 PBSC procedures on 17 patients in a multiple myeloma protocol comparing MNC yield, as well as red blood cell (RBC), granulocyte, and platelet (Plt) content, of four average WBFR groups. Standard PBSC procedures were performed using modified procedure 1, interface offset 100, anticoagulant (AC) ratio of 11:1, small volume collection chamber, and a processing time of 4 hours. After correcting for AC volume used, the volume processed was divided by 240 minutes to obtain the average WBFR. WBFRs were separated into 4 groups of 40-49, 50-59, 60-69, and 70-79 ml/min. When compared to the highest flow rate group (70-79 ml/min), the three lower flow rate groups had significantly higher MNC yields of 16.2 +/- 6.9, 13.1 +/- 5.1, and 11.5 +/- 4.7 x 10(9), respectively, as compared to 8.9 +/- 6.1 x 10(9) MNC for the 70-79 ml/min group. There was no significant difference in granulocyte yield which ranged from 1.6 +/- 2.1 to 4.5 +/- 4.8 x 10(9).(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Progenitor Cell Assaysmentioning

confidence: 99%

Effects of whole blood flow rates on mononuclear cell yields during peripheral blood stem cell collection using fenwal CS 3000 plus

Lin

Burgstaler

Pineda

et al. 1995

J of Clinical Apheresis

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“…5 2 10 6 CD34 cells/kg (Hohaus et al, 1993;Haas et al, 1995b;. Blood stem cells reconstitute hematopoiesis faster than bone marrow (Kessinger et al, 1988;Körbling et al, 1990;Hohaus et al, 1993;Bensinger et al, 1995), which might be related to differences in number and composition of haemopoietic progenitor cells. For instance, differences between both haemopoietic sources have been found with regard to the expression of several antigens, like Thy-1, c-kit, CD45RA as well as various adhesion molecules Pierelli et al, 1994;Möhle et al, 1995;Haas et al, 1995a).…”

mentioning

confidence: 99%

HIGH‐DOSE THERAPY WITH PERIPHERAL BLOOD STEM CELL TRANSPLANTATION RESULTS IN A SIGNIFICANT REDUCTION OF THE HaEMOPOIETIC PROGENITOR CELL COMPARTMENT

et al. 1996

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In order to study the effect of high-dose therapy with peripheral blood stem cell transplantation (PBSCT) on the haemopoietic reserve in man, the number and composition of bone marrow (BM) and peripheral blood (PB)-derived progenitor cells were examined in 137 cancer patients. In 45 patients, paired samples from BM and PB were obtained before PBSC mobilization and 6-27 months after transplantation. Following PBSCT. the proportion of CD34+ cells was significantly smaller than before mobilization (BM 1.99 +/- 0.24 versus 0.8 +/- 0.09, P < 0.001), and no change was observed at several follow-up visits thereafter. The reduction was most pronounced for the primitive BM progenitor subsets such as the CD34+/DR- and CD34+/ Thy-1+ cells. The impairment of hematopoiesis was also reflected by a significant reduction in the plating efficiency of BM and PB samples. No relationship was found between the decrease in the proportion of CD34+ cells and any particular patient characteristics, kind of high-dose therapy or the CD34+ cell content in the autograft. In conclusion, high-dose therapy with PBSC transplantation is associated with a long-term impairment of the haemopoietic system. The reduction in the number of haemopoietic progenitor cells is not associated with a functional deficit, as peripheral blood counts post-transplantation were normal in the majority of patients.

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“…The presence of higher numbers of primitive haemopoietic cells in a PBPC transplant may contribute to a better quality long-term reconstitution . The CFU-GM colony assay has been used to predict haemopoietic reconstitution by correlating it with numbers of mononuclear cells and CD34 þ cells (Kessinger et al, 1988;To et al, 1986To et al, , 1997Faucher et al, 1996). In some studies a strong correlation between CFU-GM colony count and CD34 þ cells was reported (Haas et al, 1995b;Millar et al, 1996), but in others no correlation was found (Serke et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

“…The classification of progenitor cells infused may assist in the more precise prediction of the engraftment period than indirect methods such as colonyforming assays. Traditionally, measurements are made using mononuclear cell count, CFU-GM assay and phenotypic evaluation of CD34 þ cells (To et al, 1986;Kessinger et al, 1988;Fruehauf et al, 1995;Haas et al, 1995b;Millar et al, 1996). None of these tests is a direct measure of stem cell content.…”

mentioning

confidence: 99%

Extensive phenotypic analysis of CD34 subsets in successive collections of mobilized peripheral blood progenitors

Perey¹,

Peters²,

Pampallona³

et al. 1998

Br J Haematol

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Summary. The transplantation of mobilized progenitor cells after high-dose chemotherapy shortens haemopoietic engraftment. CD34 cell subsets were examined in 20 consecutive mobilized progenitor cell collections obtained from patients with solid tumours that had not been previously treated. The analysis of CD34 cells was based on the expression of intracellular antigens, surface antigens including CD38, and cell size using multi-dimensional flow cytometry. We also correlated the numbers of stem cell subsets reinfused to haemopoietic recovery.The majority of CD34 þ cells expressed CD13 and CD33. A significant proportion was cytoplasmic myeloperoxidase (cMPO) positive. CD34þ MPO þ cells increased significantly in late collections. MPO expression was related to cell size. Cells expressing CD13 also increased in late collections in parallel to CFU-GM count. Small subpopulations of CD34 þ CD38þ were committed to B cells, T cells and erythroid cell lineages. A small population expressing the megakaryocytic antigen had a small size and were predominantly CD38 ¹ . A minor subpopulation expressed stem cells antigens. These were significantly higher in late collections (CD34 þ Thy-1 þ and CD34 þ CD33 ¹ ). After mobilization, patients received three cycles of intensive chemotherapy followed by reinfusion of mobilized progenitors (5·45 × 10 6 /kg CD34 þ cells, range 3·4-11·88). The numbers of reinfused CD34 cells or the individual subsets did not influence recovery of leucocytes (9 d) or platelets (9 d).In conclusion, the numbers of stem cells and their subsets differed between collections and, in unpretreated patients receiving intensive chemotherapy, there was no delayed engraftment when sufficient numbers of stem cells were reinfused. The recovery period was short and not correlated to any stem cell subsets.

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Autologous peripheral hematopoietic stem cell transplantation restores hematopoietic function following marrow ablative therapy

Cited by 360 publications

References 16 publications

Effects of whole blood flow rates on mononuclear cell yields during peripheral blood stem cell collection using fenwal CS 3000 plus

Effects of whole blood flow rates on mononuclear cell yields during peripheral blood stem cell collection using fenwal CS 3000 plus

HIGH‐DOSE THERAPY WITH PERIPHERAL BLOOD STEM CELL TRANSPLANTATION RESULTS IN A SIGNIFICANT REDUCTION OF THE HaEMOPOIETIC PROGENITOR CELL COMPARTMENT

Extensive phenotypic analysis of CD34 subsets in successive collections of mobilized peripheral blood progenitors

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