Autologous Platelet-Rich Plasma as an Adipocyte In Vivo Delivery System: Case Report

Abstract

Tissue engineering has emerged as a promising alternative to current clinical treatments for restoration of soft tissue defects. A key element in the process of tissue engineering is an ideal implant that provides structural support and a favorable environment for growing cells. The authors hypothesized that autologous platelet-rich plasma (APRP) could be used as an in vivo adipocyte delivery system to favor cell survival and to stimulate early recruitment of microcapillaries to the site of implantation. Autologous fat was included in APRP and injected as a gel into a subcutaneous pocket created to correct a painful, adherent scar at the shoulder level in a 75-year-old woman. The surgical outcome was evaluated by histologic and immunohistochemical analysis as well as by ecography before and after surgery. The results were satisfactory, showing fat survival 1 year after surgery. The characteristics of this new material should stimulate research into future clinical applications for such cell constructs in plastic and reconstructive surgery.

Application of platelet-rich plasma accelerates the wound healing process in acute and chronic ulcers through rapid migration and upregulation of cyclin A and CDK4 in HaCaT cells

“…Application of autologous PRP has been reported to facilitate wound healing in several fields, including facilitating bone proliferation in orthopedic surgery, regenerating periodontal ligaments and accelerating the wound healing process in diabetic ulcers (2)(3)(4). Therefore, in this study, we applied autologous PRP not only to surgical wounds, but also to various forms of ulcers.…”

Platelet-rich plasma induces increased expression of G1 cell cycle regulators, type I collagen, and matrix metalloproteinase-1 in human skin fibroblasts

“…In clinical applications PRP has been used to facilitate bone proliferation in nonunion fractures of joint replacement of orthopedic surgery, regeneration of periodontal ligaments, and to accelerate the wound healing process in diabetic ulcers (2)(3)(4). However, the precise molecular mechanisms of the PRP-induced wound healing process remain to be elucidated.…”

Advanced Lipotransfer Techniques

“…Princeton, New Jersey) to process 18 cc of peripheral blood. Recent studies have shown excellent results when autologous fat is combined with PRP in aesthetic plastic surgery (73,74,75). In order to increase the potential for autologous fat graft acceptance and retention we mix the harvested fat with the autologous PRP in a 4:1 ratio.…”