Autologous platelet rich plasma for regeneration of tendon injuries in horses

Roshini, S.; Arunprasad, A.; William, B. Justin; Jeyaraja, K.; Priyadharshini, Kavya

doi:10.18805/ijar.b-3653

Cited by 2 publications

(2 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…До першого зараховують плазму, збагачену тромбоцитами (PRP -Platelet-Rich Plasma) (Dohan et al, 2006;Douglas et al, 2018;Shevchenko et al, 2019). Удосконалення технологій отримання речовин, концентруючих тромбоцити, сприяло розвитку другого покоління цих біоматеріалів, а саме фібрину, збагаченого тромбоцитами (PRF -Platelet-Rich Fibrin) (Dohan Ehrenfest et al, 2012;Tina Roshini et al, 2019;Chernyshenko et al, 2019). Аутологічні продукти першого та другого покоління відрізняються один від одного за фізичними властивостями, а також за способами їх одержання, в тому числі за кількістю ступенів центрифугування (Pazzini et al, 2016;Khalaf & Salih, 2018;Yaltirik et al, 2018).…”

Section: вступunclassified

Histological characteristics of Platelet-Rich Fibrin clots obtained under various modes of blood centrifugation

Shevchenko

Rublenko

2020

SMLNUVMB

View full text Add to dashboard Cite

Autologous products of the first and second generation, namely platelet-rich plasma and platelet-rich fibrin, are considered promising for regenerative medicine. They differ from each other in physical properties, as well as in the way they are obtained. The key procedure of all techniques is centrifugation; changing its parameters affects the biological properties of these biomaterials. The aim of the work is to determine and histologically characterize the area of concentration cells of autologous fibrin enriched with platelets, depending on the change in centrifugation parameters. The studies were carried out on rabbits. Blood was collected and platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) were obtained using different values of relative centrifugal force: 100 g, 400 g, 735 g, 906 g, 1843 g. Due to the fact that it is impossible to determine the number of platelets in PRF clots, the counting was performed in platelet-rich plasma obtained by a single centrifugation with the corresponding parameters that were used to obtain PRF. The length of the formed clots was compared and a histological assessment of the cell composition in different layers (lower, middle and upper) was carried out. The highest platelet concentrations were observed in PRP obtained at 100 g and 400 g. Application of different values of centrifugal force showed obvious differences in the formation of platelet-rich fibrin clots. After preparation of I-PRF, its volume was significantly less than that of standard PRF, and the border between erythrocytes was less distinct. During the histological examination of fibrin clots, a change in the distribution of cellular elements in different parts was found with a change in the centrifugation parameters. With an increase in the parameter of relative centrifugal force, the length of the fibrin clot significantly increases, but the concentration of platelets in it significantly decreases. That is, it was found that the most optimal value of the relative centrifugal force for obtaining platelet mass is 100 g, which makes it possible to achieve the number of platelets greater than 800×109/L.

show abstract

Section: вступunclassified

Histological characteristics of Platelet-Rich Fibrin clots obtained under various modes of blood centrifugation

Shevchenko

Rublenko

2020

SMLNUVMB

View full text Add to dashboard Cite

show abstract

“…Similarly, it has been reported in several clinical studies that growth factors play a key role in the repair of hard and soft tissue, thus topical treatments facilitate wound healing in normal tissue and heal persistent non-healing wounds (Debus et al 2000;Tahir et al, 2018). They are also efficiently used in skin lesions, urology, orthopedics and plastic surgery, ophthalmology, dental surgery and the repair of various tissues (Parafioriti et al, 2011;Chung et al, 2015;Roshini et al, 2019;Sardari et al, 2011;Carter et al, 2011).…”

Section: Introductionmentioning

confidence: 93%

The Effect of Platelet Rich Plasma on Wound Healing in the Repair of Experimental Penile Fractures

Beğer

Sağlam

Düz

et al. 2021

IJAR

View full text Add to dashboard Cite

Background: Penile fractures are caused by the rupture of the tunica albuginea and the underlying layers of corpus cavernosum as a result of the exposure of the erect penis to blunt trauma. Although conservative treatment methods have been recommended in former studies, recent studies favor early surgical options. Platelet-rich plasma (PRP) is a blood product rich in cytokines and several growth factors, obtained from the patient’s own blood commonly used to stimulate healing in acute and chronic wound treatments. The efficiency of homologous PRP treatment in rats with penile fractures have been assessed in terms of clinical manifestation and histopathological findings in the present study. Methods: Thirty-five rats were randomly divided into five groups, each containing seven rats as follows: 1. Conservative group: Following the formation of penile fracture, medical interventions were not initiated on fracture area and the wound was left for secondary healing. 2. Bandage group: Following the formation of penile fracture, self-adherent elastic bandage (3M Coban) was applied to the fracture area for three days. 3. Primary repair group: Following the formation of a penile fracture, the lacerated area was repaired with 7/0 vicryl primary sutures. 4. Primary repair + Homologous Platelet Rich Plasma group: Before the operation, 10 cc of blood was taken from one rat under general anesthesia by intracardiac puncture and PRP material was prepared from this blood. Homologous PRP was topically administered on the wound in the fracture area and then the penile fracture was repaired with 7/0 vicryl as simple separate stitches. 5. Non-operative control group: Surgical procedures were not applied to this group; it was used as the control group. Result: The effect of the PRP treatments on the surgical repair options in the rats with penile fractures was investigated. The present findings revealed that following the repair with conservative treatments, the fracture format persisted and various complications like infection, oedema, excessive fibrosis and hyperemia were encountered. On the other hand, the best healing was achieved in primary repair + PRP group in terms of clinical manifestation and histopathological findings.

show abstract

Autologous platelet rich plasma for regeneration of tendon injuries in horses

Cited by 2 publications

References 32 publications

Histological characteristics of Platelet-Rich Fibrin clots obtained under various modes of blood centrifugation

Histological characteristics of Platelet-Rich Fibrin clots obtained under various modes of blood centrifugation

The Effect of Platelet Rich Plasma on Wound Healing in the Repair of Experimental Penile Fractures

Contact Info

Product

Resources

About

Autologous platelet rich plasma for regeneration of tendon injuries in horses

Cited by 2 publications

References 32 publications

Histological characteristics of Platelet-Rich Fibrin clots obtained under various modes of blood centrifugation

Histological characteristics of Platelet-Rich Fibrin clots obtained under various modes of blood centrifugation

​The Effect of Platelet Rich Plasma on Wound Healing in the Repair of Experimental Penile Fractures

Contact Info

Product

Resources

About

The Effect of Platelet Rich Plasma on Wound Healing in the Repair of Experimental Penile Fractures