Histological characteristics of Platelet-Rich Fibrin clots obtained under various modes of blood centrifugation

Abstract

Autologous products of the first and second generation, namely platelet-rich plasma and platelet-rich fibrin, are considered promising for regenerative medicine. They differ from each other in physical properties, as well as in the way they are obtained. The key procedure of all techniques is centrifugation; changing its parameters affects the biological properties of these biomaterials. The aim of the work is to determine and histologically characterize the area of concentration cells of autologous fibrin enriched with platelets, depending on the change in centrifugation parameters. The studies were carried out on rabbits. Blood was collected and platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) were obtained using different values of relative centrifugal force: 100 g, 400 g, 735 g, 906 g, 1843 g. Due to the fact that it is impossible to determine the number of platelets in PRF clots, the counting was performed in platelet-rich plasma obtained by a single centrifugation with the corresponding parameters that were used to obtain PRF. The length of the formed clots was compared and a histological assessment of the cell composition in different layers (lower, middle and upper) was carried out. The highest platelet concentrations were observed in PRP obtained at 100 g and 400 g. Application of different values of centrifugal force showed obvious differences in the formation of platelet-rich fibrin clots. After preparation of I-PRF, its volume was significantly less than that of standard PRF, and the border between erythrocytes was less distinct. During the histological examination of fibrin clots, a change in the distribution of cellular elements in different parts was found with a change in the centrifugation parameters. With an increase in the parameter of relative centrifugal force, the length of the fibrin clot significantly increases, but the concentration of platelets in it significantly decreases. That is, it was found that the most optimal value of the relative centrifugal force for obtaining platelet mass is 100 g, which makes it possible to achieve the number of platelets greater than 800×109/L.

Clinical and Histopathological Studies on the Efficacy of Multiple Injections of I-PRF Versus the Single Use of A-PRF in Repair of Achilles Tendon Rupture in Dogs: An Experimental Study

“…Histological evaluation revealed that the multiple use of I-PRF promoted better healing of the tendon. At the 6 th week observation, histological evaluation revealed better cell migration, proliferation and density as well as better arrangement and condensation of the fibers in the I-PRF group than A-PRF group while at the 12 th week observation showed higher condensation and arrangement of the fiber content with higher degree of collagen expression and lower cellularity indicating faster healing and regeneration of I-PRF group than A-PRF group and that was theoretically due to the continuous release of the GFs, cytokines and presence of leukocytes that was evident in the I-PRF because of the lowspeed centrifugation that prevented the push of these cells and permitted their presence in the I-PRF liquid during its preparation and that agreed with (Ghanaati et al 2014, Shevchenko andRublenko 2020).…”

“…Recent studies proved the efficacy of I-PRF for enhancement of the healing process in different tissues (Wang et al 2018). Abd El Raouf et al (2019demonstrated for the first time the superior cartilage regeneration potential of I-PRF using the low-speed centrifugation concept in the early stages of cartilage repair while Shevchenko and Rublenko (2020) proved the higher concentration of platelets and leukocytes of the I-PRF in their study. This study aimed for evaluation of the efficacy of both I-PRF and A-PRF in enhancement of the healing of surgically treated AT rupture in dogs and compare the efficacy of the single administration of the A-PRF membranes with multiple injections of the I-PRF.…”

Histomorphological characteristics of bone replacement in rabbits with hydroxyapatite ceramics and Platelet-Rich Fibrin

“…Попередньо нами (Shevchenko et al, 2019;Shevchenko, 2020;Shevchenko & Rublenko, 2020), було проведено моніторингову оцінку технологій одержання тромбоцитарних мас для регенеративної медицини, їх гістологічну характеристику, встановлено динаміку гематологічних показників, макроморфологічної і рентгенологічної картини репаративного остеогенезу в кролів за використання тромбоцитарних концентратів та гідроксиапатитної кераміки, що засвідчили перспективність цього напряму.…”