Autologous skin cell spray-transplantation for a deep dermal burn patient in an ambulant treatment room setting

Gerlach, Jörg C.; Johnen, Christa; McCoy, Eric; Bräutigam, Kirsten; Plettig, Jörn; Corcos, A

doi:10.1016/j.burns.2011.01.022

Cited by 48 publications

(30 citation statements)

References 24 publications

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“…The provided wound irrigation can support either the cells present in the wound or the additionally sprayed autologous cells following a cell spray transplantation (19)(20)(21) (Fig. 1).…”

Section: Thoughts and Progressmentioning

confidence: 99%

“…The cells are taken from a healthy area of the patient's own skin and expanded in culture dishes into cell sheets, which are then transferred to the patient as confluent and stratified multilayer sheets to cover large-area burns when donor sites are limited. Wood et al therefore introduced single-cell spray grafting of noncultured cells, which we implemented into our clinical practice (19,20). Disadvantages of these cultured keratinocyte sheets include long in vitro expansion times of 2-4 weeks to obtain the required cell number, while the differentiation of basal keratinocyte skin progenitor cells takes place in the in vitro situation (15)(16)(17)(18).…”

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confidence: 99%

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Active Wound Dressing With Artificial Capillaries for Temporary Wound Irrigation and Skin Cell Supply

Plettig¹,

Johnen²,

Bräutigam³

et al. 2011

Artificial Organs

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Medical treatment of burns and chronic wounds remains a challenge. We discussed a therapy concept that combines skin cell spray transplantation with a novel wound dressing based on artificial hollow fiber membrane capillaries. In skin cell-based therapy development, autologous skin progenitor cells are isolated from a healthy skin area and sprayed onto the wound. A medical device was introduced that uses perfused capillaries, known from clinical plasma separation, as a temporarily applied extracorporeal wound capillary bed. The functions of the dressing are comparable with those of dialysis; the capillaries, however, are applied externally onto the wound. Perfusion with a clinical peripheral nutrition and buffer solution can provide wound irrigation, wound debris removal, cell nutrition, pH regulation, and electrolyte balance while potentially serving to address delivery of regenerative factors and antibiosis. An innovative active skin wound dressing that provides cell support and stimulates regeneration by wound irrigation is discussed.

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“…The provided wound irrigation can support either the cells present in the wound or the additionally sprayed autologous cells following a cell spray transplantation (19)(20)(21) (Fig. 1).…”

Section: Thoughts and Progressmentioning

confidence: 99%

mentioning

confidence: 99%

Active Wound Dressing With Artificial Capillaries for Temporary Wound Irrigation and Skin Cell Supply

Plettig¹,

Johnen²,

Bräutigam³

et al. 2011

Artificial Organs

Self Cite

View full text Add to dashboard Cite

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“…This would be expected to result in a thin and weaker dermis that would be predisposed to re-ulceration in the face of renewed insult, just as the skin of the aged or persons suffering from inanition is ripe for ulcers. The implications of such crosstalk, with the closing epidermis signaling ‘stop repair’ signals to the dermis, must be considered when using new re-epithelialization technologies such as keratinocyte transplantation (73). …”

Section: Matrix In Dysrepairmentioning

confidence: 99%

“…More recently, cellular transplants often in the form of keratinocytes have been proposed as a rapid covering for burn wounds (73) and venous stasis ulcers (90). While both compassionate use and a phase II trial have suggested some benefit, that even persists at six months post closure (91), the healed wounds have not been studied in detail.…”

Section: Model Systems and Matrix Interventionsmentioning

confidence: 99%

Skin tissue repair: Matrix microenvironmental influences

2016

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The process of repair of wounded skin involves intricate orchestration not only between the epidermal and dermal compartments but also between the resident and immigrant cells and the local microenvironment. Only now are we beginning to appreciate the complex roles played by the matrix in directing the outcome of the repair processes, and how this impacts the signals from the various cells. Recent findings speak of dynamic and reciprocal interactions that occurs among the matrix, growth factors, and cells that underlies this integrated process. Further confounding this integration are the physiologic and pathologic situations that directly alter the matrix to impart at least part of the dysrepair that occurs. These topics will be discussed with a call for innovative model systems of direct relevance to the human situation.

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“…Suitability of cell spraying was already shown for external/superficial applications e.g. treatment of burn injuries by [5], treatment of topographically challenging wounds by [6] and most recently in [7]. However, application of cell-sprays within the human body was not realized so far.…”

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confidence: 99%

Viability of coaxial atomization for disintegration of cell solutions in cell spray applications

Bieber

Menzel

Thiebes

et al. 2017

Proceedings ILASS–Europe 2017. 28th Conference on Liquid Atomization and Spray Systems

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Treating Leukemia with intravenous stem cell transplantation represents a well-established therapy technique. For applications, that require high local cell concentrations, transplantation by conventional intravenous injection is less potent, due to cell distribution with blood circulation. Instead, spraying them directly onto the injured or diseased area shows promising results in various applications, e.g. superficial treatment of topographically challenging wounds, in situ seeding of cells on implants, deposition of cells in tubular organs for stem cell therapy. The present work aims for a basic knowledge about viability boundaries for coaxial cell-spray atomization and the reciprocal influence between cells in solution and primary breakup mechanics. A generic modular nozzle is developed, to ensures reproducible boundary conditions. Investigations are conducted regarding primary breakup and relations between resulting droplet size distribution and cell survival. Measurements are performed, utilizing microscopic high-speed visualization with suitable image post processing. Cell viability is analyzed using phase contrast microscopy prior and after atomization. A relation between Rayleigh-Taylor instability wavelength and droplet size distributions by means of Sauter mean diameter (SMD) and cell survival rate (CSR) is suggested. A power law is presented, exclusively dependent on dimensionless measures (λ ⊥ ∼ Re −1/2 We −1/3 ) which is found to be proportional to SMD and CSR. Keywords cell spray, coaxial atomization, cell survival IntroductionStem cell therapy is a research field in regenerative medicine with increasing importance due to the ability of cells to support healing of afflicted tissue. A well-established example represents the treatment of Leukemia by means of intravenous stem cell transplantation, where cells distribute by blood circulation within the organism after injection. To utilize the full potential of stem cells for applications that require high local cell concentration, alternative approaches for transplantation would be desirable, that enable local treatment rather than homogeneous distribution. An example for the requirement of high concentrations is the treatment of Acute Lung Injury (ALI) or Acute Respiratory Distress Syndrome (ARDS), both resulting from inhalation of biological toxins or toxic chemicals [1]. Despite extensive research, the mortality rate remains high (40%) for both diagnoses [2]. As stated in [3], the anti-inflammatory properties of intrapulmonary instilled MSCs decrease the severity of endotoxin-induced ALI and improves survival in animal studies, though the increased liquid feed may further damage the lung tissue. Minimization of liquid feed into the lung could be achieved by using droplets as a carrier medium. A well-established technique for drug delivery in the lung using droplets, is the nebulization of liquids into a fine mist, that is directly inhaled. However, nebulization of cell solutions leads to high cell mortality rates [4]. In addition, direct in...

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Autologous skin cell spray-transplantation for a deep dermal burn patient in an ambulant treatment room setting

Cited by 48 publications

References 24 publications

Active Wound Dressing With Artificial Capillaries for Temporary Wound Irrigation and Skin Cell Supply

Active Wound Dressing With Artificial Capillaries for Temporary Wound Irrigation and Skin Cell Supply

Skin tissue repair: Matrix microenvironmental influences

Viability of coaxial atomization for disintegration of cell solutions in cell spray applications

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