Recently, automatic diagnosis of diabetic retinopathy (DR) from the retinal image is the most signi¯cant research topic in the medical applications. Diabetic macular edema (DME) is the major reason for the loss of vision in patients su®ering from DR. Early identi¯cation of the DR enables to prevent the vision loss and encourage diabetic control activities. Many techniques are developed to diagnose the DR. The major drawbacks of the existing techniques are low accuracy and high time complexity. To overcome these issues, this paper proposes an enhanced particle swarm optimization-di®erential evolution feature selection (PSO-DEFS) based feature selection approach with biometric authentication for the identi¯cation of DR. Initially, a hybrid median lter (HMF) is used for pre-processing the input images. Then, the pre-processed images are embedded with each other by using least signi¯cant bit (LSB) for authentication purpose. Simultaneously, the image features are extracted using convoluted local tetra pattern (CLTrP) and Tamura features. Feature selection is performed using PSO-DEFS and PSO-gravitational search algorithm (PSO-GSA) to reduce time complexity. Based on some performance metrics, the PSO-DEFS is chosen as a better choice for feature selection. The feature selection is performed based on the¯tness value. A multi-relevance vector machine (M-RVM) is introduced to classify the 13 normal and 62 abnormal images among 75 images from 60 patients. Finally, the DR patients are further classi¯ed by M-RVM. The experimental results exhibit that the proposed approach achieves better accuracy, sensitivity, and speci¯city than the existing techniques.