Automated egg counting and sizing from scanned images: Rapid sample processing and large data volumes for fecundity estimates

Friedland, Kevin D.; Ama-Abasi, Daniel; Manning, M.; Clarke, L.; Kligys, G.; Chambers, R. Christopher

doi:10.1016/j.seares.2005.06.002

Cited by 57 publications

(46 citation statements)

References 28 publications

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“…The relationship also differed from the relationship in Thorsen and Kjesbu (2001), which consisted of a power curve through the data, whereas in the present study, there was an exponential regression line. Friedland et al (2005) concluded that the relationship between follicle size and density in American shad (Alosa sapidissima) was too imprecise to provide useful fecundity estimates. They suggested that the poor precision may be due to their sample handling and preservation technique but also may reflect species differences in ovarian development and ovarian anatomy, which may be why there is a difference between the relationship for the present study and that of Thorsen and Kjesbu (2001).…”

Section: Discussionmentioning

confidence: 99%

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The concept of fecundity regulation in plaice (Pleuronectes platessa) tested on three Irish Sea spawning populations

Kennedy¹,

Witthames²,

Nash³

2007

Can. J. Fish. Aquat. Sci.

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Abstract:The fecundity of European plaice (Pleuronectes platessa) in the Irish Sea between 2000 and 2004 was estimated during the spawning season for fish in the three main spawning areas (Liverpool Bay, the Cumbrian coast, and the western Irish Sea) and one small spawning group on the west coast of the Isle of Man. Fecundity was also estimated during September of 2003 and 2004. The aim of this was to assess the variability in fecundity between areas and years in the Irish Sea and also to identify when differences in fecundity become apparent in the maturation cycle. There were variations in fecundity on both the temporal and spatial scales. The greatest variation in fecundity between years occurred in the western Irish Sea, whereas there was no variation between years in the southeastern Irish Sea (Liverpool Bay). There was no difference in fecundity between areas or years during September. The maximum fecundity in plaice is determined by the total weight of the fish at the end of follicle recruitment in the ovary, and differences in the fecundity of each population are the result of different levels of down-regulation in the period between the end of follicle proliferation and spawning.

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Section: Discussionmentioning

confidence: 99%

“…Previous fecundity studies have used Gilson's to preserve the ovaries and break down the ovarian tissue (Nash et al 2000;Friedland et al 2005), whereas during the present study, we use formaldehyde. Gilson's is a digestive fluid that is much more toxic and causes greater shrinkage in follicle size than formaldehyde.…”

Section: Discussionmentioning

confidence: 99%

The concept of fecundity regulation in plaice (Pleuronectes platessa) tested on three Irish Sea spawning populations

Kennedy¹,

Witthames²,

Nash³

2007

Can. J. Fish. Aquat. Sci.

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“…These oocytes were in the vitellogenic stage where they have a brownish colour and therefore easily stand out from a bright background in contrast to fertilized eggs which are transparent. Friedland et al (2005) developed an image analysis method for scanned images of fish eggs in Petri dishes. This method first transfixes the eggs in a polymer before they are scanned.…”

Section: Introductionmentioning

confidence: 99%

An automatic counting system for transparent pelagic fish eggs based on computer vision

Duan

Stien

Thorsén

et al. 2015

Aquacultural Engineering

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“…The main problem with alcohol solutions such as 70% EtOH (EtOH-70), which is one of the most common preservatives of animal tissue, is that they can cause severe tissue dehydration and could thus be inappropriate for methods that require accurate measurements of gonad and/or oocyte size. Moreover, other ethanol-based, formalin-free protocols such as the toxic Gilson's fluid or its modified non-toxic alternative proposed by Friedland et al (2005), which are used to separate oocytes from ovarian tissue for fecundity measurements, were also shown to cause significant oocyte shrinkage (Friedland et al, 2005;Klibansky & Juanes, 2007).…”

Section: Introductionmentioning

confidence: 99%

Assessing species and stage specific effects of preservation on fish oocytes over different temporal scales

Rakka

Ganias

2015

Medit. Mar. Sci.

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This study assessed the effect of 10% neutral buffered formalin and three ethanol solutions of different concentration on Mediterranean sardine and European anchovy oocytes over several temporal scales (days, weeks, months). The two species exhibit differences both in the elemental composition and the shape of their oocytes, which allowed an appraisal of oocyte shrinkage dynamics in relation to oocyte shape, developmental stage and composition. We showed that the effect of the preservative on oocyte size is stage-specific while different preservation periods of ovarian material might lead to discrepancies among studies.

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Automated egg counting and sizing from scanned images: Rapid sample processing and large data volumes for fecundity estimates

Cited by 57 publications

References 28 publications

The concept of fecundity regulation in plaice (Pleuronectes platessa) tested on three Irish Sea spawning populations

The concept of fecundity regulation in plaice (Pleuronectes platessa) tested on three Irish Sea spawning populations

An automatic counting system for transparent pelagic fish eggs based on computer vision

Assessing species and stage specific effects of preservation on fish oocytes over different temporal scales

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