Automated Microarray System for the Simultaneous Detection of Antibiotics in Milk

Knecht, Bertram G; Strasser, Alexandra; Dietrich, Richard; Märtlbauer, Erwin; Nießner, Reinhard; Weller, Michael G.

doi:10.1021/ac035028i

Cited by 238 publications

(134 citation statements)

References 38 publications

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“…Such antibodies would be potentially useful for detecting multiple toxins on a microarray [16,17] and for developing antibody-based multidimensional analytical methods for the determination of cross-reacting toxins as accomplished elsewhere [18][19][20]. However, this is hardly possible by using only the conventional immunogen AFB1-CMO-protein.…”

Section: Afb1mentioning

confidence: 99%

Novel Aflatoxin Derivatives and Protein Conjugates

et al. 2007

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Aflatoxins, a group of structurally related mycotoxins, are well known for their toxic and carcinogenic effects in humans and animals. Aflatoxin derivatives and protein conjugates are needed for diverse analytical applications. This work describes a reliable and fast synthesis of novel aflatoxin derivatives, purification by preparative HPLC and characterisation by ESI-MS and one-and two-dimensional NMR. Novel aflatoxin bovine serum albumin conjugates were prepared and characterised by UV absorption and MALDI-MS. These aflatoxin protein conjugates are potentially interesting as immunogens for the generation of aflatoxin selective antibodies with novel specificities.

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Section: Afb1mentioning

confidence: 99%

Novel Aflatoxin Derivatives and Protein Conjugates

et al. 2007

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“…The analysis of the spatial pattern of the light output, measured via an imaging charge-coupled device (CCD) device, allowed the detection of the different analytes [117]. Recent work includes the parallel affinity sensor assay (PASA) which used automated chip-multi-analyte IA with an indirect competitive ELISA format and a CL reaction monitored by a charge-coupled device (CCD) camera for the simultaneous detection of antibiotics in milk [118] and allergy diagnosis [119], and the development of miniaturized CE separative system with CL detection using miniaturized microchip fabrication technology [120].…”

Section: Future Outlookmentioning

confidence: 99%

Chemiluminescence immunoassay

Zhao

Sun

Chu

2009

TrAC Trends in Analytical Chemistry

193

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Chemiluminescence immunoassay (CLIA) offers several potential advantages and has applications in clinical chemistry, bioanalysis and environmental analysis. This review covers recent developments and applications of CLIA in immunorecognition, CL markers and related techniques, and solid-phase materials. We describe automation, integration and miniaturization of CLIA. We evaluate different CLIA systems and give some pointers as to the likely directions of future developments. ª

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“…Knecht et al 102 presented a parallel affinity sensor array (PASA) for the rapid automated analysis of 10 antibiotics in milk, by immobilizing protein conjugates of the haptens on disposable chips. The CL signal was recorded with a sensitive CCD camera.…”

Section: ·1·3 Microfluidic Biochipmentioning

confidence: 99%

Chemiluminescence Platforms in Immunoassay and DNA Analyses

Fan

Cao

et al. 2009

ANAL. SCI.

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IntroductionCL is the production of electromagnetic radiation by a chemical reaction. The CL intensity is directly proportional to the concentration of a limiting reactant involved in the CL reaction. CL has been exploited for its use in a wide range of applications due to its high sensitivity, wide calibration range, and suitability for miniaturization. Protein and DNA analyses have recently attracted much attention. Many different types of techniques, such as electrochemical, optical, radiochemical, and piezoelectronic methods have been applied for protein and DNA analyses. To provide an overview of CL detection assays for DNAs and proteins, we have summarized their essential techniques. Monoplex and multiplex assays are described, and their current reports are intended to provide an overview of the CL techniques currently employed in numerous laboratories. Monoplex AssayA monoplex assay aims to measure the presence of a single analyte in any given sample, in which nanoparticle, DNA enzyme, horseradish peroxidase (HRP), alkaline phosphatase (AP) and acridinium ester are commonly used as labels. 2·1 Nanoparticle-based CL techniquesNanoparticles offer excellent prospects for DNA detection and immunoassay, owing to their many attractive properties. Compared to existing labels, nanoparticles are more stable and cheaper, and easier to be purified. In addition, they indicate faster binding kinetics with high sensitivity and a high reaction rate for many types of monoplex assays, ranging from immunoassays to DNA detection. 2·1·1 Gold nanoparticlesColloidal gold labels are ideal in biotechnological systems for several reasons. Firstly, they can be readily prepared in a wide range of sizes, from approximately 2 nm to above 100 nm. Secondly, the biochemical activity of the labeled biomolecules can be retained when colloidal gold is coupled to the biomolecules and, thirdly, colloidal gold labels can be easily visualized as dense structures within biological entities using transmission electron microscopy. As a biological tag, colloidal gold has been employed in several CL detection systems. 2·1·1·1 Gold nanoparticle-based CL immunoassay. The Au 3+ -luminol reaction, in which 10 -9 M Au 3+ ions can be sensitively detected, is a classic CL reaction. Thousands of Au atoms are contained within the gold nanoparticles; for example, 2.3 ¥ 10 5 Au atoms are theoretically contained in a 20-nm spherical gold particle and, consequently, picomolar detection limits can be attained by employing oxidative gold metal dissolution in acidic solutions. Our group 1,2 reported a sensitive CL immunoassay based on a colloidal gold label after oxidative gold metal dissolution (using 0.01 M HCl-0.5 M NaCl-0.5 mM Br2), using a simple and sensitive luminol-CL reaction (Fig. 1). Reviews Chemiluminescence Platforms in Immunoassay and DNA AnalysesAiping FAN,* Zhijuan CAO,* Huan LI,* Masaaki KAI,** and Jianzhong LU* † *School of Pharmacy, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China ** Faculty of Pharmaceutical Sciences, Graduate Scho...

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Automated Microarray System for the Simultaneous Detection of Antibiotics in Milk

Cited by 238 publications

References 38 publications

Novel Aflatoxin Derivatives and Protein Conjugates

Novel Aflatoxin Derivatives and Protein Conjugates

Chemiluminescence immunoassay

Chemiluminescence Platforms in Immunoassay and DNA Analyses

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