Automated microfluidic compact disc (CD) cultivation system of Caenorhabditis elegans

Kim, Nahui; Dempsey, Catherine; Zoval, Jim; Sze, Ji Ying; Madou, Marc

doi:10.1016/j.snb.2006.06.026

Cited by 74 publications

(63 citation statements)

References 27 publications

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“…Moreover, some of the genes and compounds were found to exhibit same effects on human beings. Microfluidic device is quite biocompatible for these long-term studies and capable to obtain the information of individual worms in developing process [25,30,42,45,46]. Clausell-Tormos et al [42] encapsulated worm eggs into aqueous droplet in a gas-permeable PTFE tubing, and different stages including egg hatching, worm growing and egg laying have been observed within the droplet ( Figure 4(a)).…”

Section: Lifespan and Developmentmentioning

confidence: 99%

Analysis of Caenorhabditis elegans in microfluidic devices

Wen

Qin

2012

Sci. China Chem.

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Caenorhabditis elegans (C. elegans) is widely adopted as a model organism for a variety of biological studies including development, genetics and neurobiology. Micro-scale microfluidic technology is capable of handling single or populations of C. elegans in high throughput format and allows for the precise spatial and temporal control of their environment, which is well suited for the study of worms in different aspects. In this review, we highlight the recent advances in microfluidic technology for the analysis of worms ranging from behavioral studies to neurobiology. We believe that microfluidic device can further be applied to study the different aspects of worms, extending from fundamental investigation of behavioral dynamics to more complicated biological processes including neurochemistry and learning behaviors. microfluidic, Caenorhabditis elegans, behavior, neurobiology, aging

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Section: Lifespan and Developmentmentioning

confidence: 99%

Analysis of Caenorhabditis elegans in microfluidic devices

Wen

Qin

2012

Sci. China Chem.

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“…(4) Multiple functional units can be assembled and diverse manipulations of worms can be processed automatically and sequentially. At present, several worm manipulation methods offered by channel-microfluidics have been proposed such as worm immobilization [21][22][23][24][25][26][27][28], cultivation (gas/liquid) [29][30][31], controllable stimulation [32][33][34][35][36][37], screening [28,38], locomotion sensing [39,40], etc.…”

Section: Elegans Manipulation In Microchannel Formatmentioning

confidence: 99%

“…Channel microfluidics provides a potential platform for the long term study of worms and this is mainly due to the capability to enable the exchange of nutrition and excreta of worms in microchannels, and the gas permeability offered by the PDMS material for necessary oxygen supply to the worms. Kim et al [29] and Hulme et al [30] realized long-term culture of C. elegans on microfluidic chips. The former researchers established a microfluidic cultivation system in which centrifugal force was utilized to drive liquid, and the processes of feeding and waste removal were accomplished automatically.…”

Section: Long-term Cultivationmentioning

confidence: 99%

Microfluidic Platform for the Study of Caenorhabditis elegans

Shi

Wen

Lin

et al. 2011

Topics in Current Chemistry

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Caenorhabditis elegans is a well-established model organism for the study of various biological areas. However, the procedures generally used for this worm are limited by the low throughput, low automation, and imprecise delivery of external stimuli. Microfluidics technology is emerging as an attractive and enabling platform to overcome these problems. In this review, we summarize the current microfluidic approaches for the investigation of behavior and neurobiology in C. elegans, and discuss the trends of future development.

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“…Microfluidics-based C. elegans studies have been carried out for maze exploration, 6 oxygen sensation, 7 phenotype and genetic screenings, 8,9 automatic cultivation, 10 and individual movement assay. 11 In addition, some microfluidic systems, which utilized the microfabricated tapered channels 12,13 or microvalves [14][15][16][17] for imaging analysis, have been reported as well.…”

Section: Introductionmentioning

confidence: 99%

A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans

Jiang

Shi

et al. 2009

Biomicrofluidics

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The soil dwelling nematode Caenorhabditis elegans ͑C. elegans͒ is an excellent model organism for the study of numerous disease including neurodegenerative disease. In this study, a programmable microvalve-based microfluidic array for real-time and long-term monitoring of the neurotoxin-induced responses of the individual C. elegans was developed. The device consisted of a flow layer and a control layer, which were used for worm manipulation. By activating the programmable microvalves in the control layer, mutiple worms could be individually captured and intermittently immobilized in parallel channels. Thus the mobility behavior, together with the corresponding dopaminergic neuron features of the worms in response to neurotoxin, could be investigated simultaneously. It was found that the neurotoxin MPP+ enabled to induce mobility defects and dopaminergic neurons loss in worms. The established system is easy and fast to operate, which offers not only the controllable microenvironment for analyzing the individual worms in parallel, monitoring the same worm over time, but also the capability to characterize the mobility behavior and neuron features in response to stimuli simultaneously. In addition, the device enabled to sustain the worm culture over most of their adult lifespan without any harm to worm, providing a potential platform for lifespan and aging research.

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Automated microfluidic compact disc (CD) cultivation system of Caenorhabditis elegans

Cited by 74 publications

References 27 publications

Analysis of Caenorhabditis elegans in microfluidic devices

Analysis of Caenorhabditis elegans in microfluidic devices

Microfluidic Platform for the Study of Caenorhabditis elegans

A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans

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