Automated multiplex nucleic acid tests for rapid detection of SARS-CoV-2, influenza A and B infection with direct reverse-transcription quantitative PCR (dirRT-qPCR) assay in a centrifugal microfluidic platform

Abstract

Development of a microfluidic disc-direct reverse-transcription quantitative PCR platform to perform automated multiplex nucleic acid tests for rapid multiplex detection of disease infection.

Viral coinfections in COVID‐19

“…[77][78][79] Due to the similarities between the clinical manifestations of SARS-CoV-2 and the influenza virus, coinfection of these two viruses may potentially disrupt the patient response to therapeutic agents for either infection, thus a simultaneous diagnosis of these viruses is necessary to provide effective care. 78 1.…”

“…18 In contrast, nucleic acid amplification test would be a suitable method for detection of influenza virus RNA in the case of coinfection with SARS-CoV-2. 78 Similarly, a proper diagnosis of DENV and SARS-CoV-2 coinfection in countries endemic to DF can only be made through the adoption of reliable laboratory tests, such as the PCR of the nasopharyngeal swab and anal swab, NS1 DENV, immunoglobulin (Ig) M and IgG assays. 187 It is also necessary to develop new strategies to better understand the clinical signs of coinfections and discover suitable therapeutic options for them.…”

A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores

“…Many devices for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection have been introduced using a range of different amplification and detection methods ( Ji et al, 2020 ; Jin et al, 2020 ; Vandenberg et al, 2020 ). To date, reverse transcription polymerase chain reaction (RT-qPCR) ( Smyrlaki et al, 2020 ; Tahamtan and Ardebili, 2020 ; Xiao et al, 2020 ) and antibody-based detection ( Cady et al, 2020 ; Vandenberg et al, 2020 ) have been applied clinically for COVID-19 detection.…”

An overview of molecular biology and nanotechnology based analytical methods for the detection of SARS-CoV-2: promising biotools for the rapid diagnosis of COVID-19

“…An automated-microfluidic disc-direct RT-qPCR assay was developed for the detection of multiple viral species (SARS-CoV-2, and influenza A and B) in pharyngeal swab samples. 46 This method required viral RNAs in the range of 2 × 10 0 to 2 × 10 6 copies per reaction and successfully detected multiple viral RNA without any sample pre-treatment. The authors achieved an impressive detection limit of 2 × 10 1 copies per reaction for SARS-CoV-2.…”