Automated Radiometric Detection of Bacteria in 2,967 Blood Cultures

DeBlanc, Harold J.; DeLand, Frank H.; Wagner, Henry N.

doi:10.1128/aem.22.5.846-849.1971

Cited by 49 publications

(42 citation statements)

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“…In recent years, studies have appeared reporting the radiometric detection of bacteremia. DeLand and Wagner (2) first reported a technique for the radiometric detection of microorganisms in blood cultures and subsequently described an automated device for this purpose (3). In a number of clinical studies, they found the radiometric method to be faster than conventional techniques and comparable in accuracy (1,3).…”

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confidence: 99%

Comparison of the BACTEC System with Blind Subculture for the Detection of Bacteremia

Caslow

Ellner

Kiehn

1974

Applied Microbiology

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One thousand blood specimens were cultured in BACTEC vials containing modified Columbia broth in aerobic, anaerobic, and hypertonic formulations. Radiometric readings and subcultures were performed on aerobic and hypertonic vials at 24 h and 7 days, and on anaerobic vials at 48 h and 7 days. Significant numbers of false-positive BACTEC readings were obtained. Although all positive cultures were eventually detected by the BACTEC, approximately 20% of blood specimens yielding positive subcultures at 24 h did not give positive BACTEC readings until 48 h.

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confidence: 99%

Comparison of the BACTEC System with Blind Subculture for the Detection of Bacteremia

Caslow

Ellner

Kiehn

1974

Applied Microbiology

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“…Recent reports (1,6) have suggested that a radiometric method is comparable in accuracy and is faster than some of the conventional techniques currently being used for the detection of bacteremia. A previous publication (5) from this laboratory reported a limited clinical comparison of a radiometric method of detecting bacteremia with the standard laboratory method.…”

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Evaluation of Radiometric System for Detecting Bacteremia

Renner¹,

Gatheridge²,

Washington³

1973

Applied Microbiology

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An automated radiometric system (BACTEC, Johnston Laboratories) for detection of bacteremia was evaluated in parallel with a standard blood culture system in use in our laboratory. Of 1,445 blood cultures from 484 patients with possible bacteremia, 106 sets of cultures (excluding 39 presumed contaminated), representing 56 patients, were positive by both methods. The conventional system yielded 85 positive cultures from 48 patients, whereas the BACTEC system yielded 84 positive cultures from 43 patients. The BACTEC system failed to detect 22 cultures that were positive in the conventional system, and the conventional system failed to detect 21 cultures that were positive in the BACTEC system. The detection efficiency was generally equivalent in the two systems except for the lower detection rates of anaerobes and Enterobacter aerogenes by the BACTEC system and the lower detection rates of Torulopsis glabrata and, possibly, Pseudomonas sp. (group IVD) in the conventional system. The BACTEC system had a slight advantage over the conventional system in the time interval to detection of positivity. Approximately 20% of the positive cultures detected by the BACTEC system were detected on the first day of incubation compared with 7% by the conventional system. The recovery rates and detection times of anaerobes were less efficient by the BACTEC system than by the conventional system. It does not appear that the radiometric method has much advantage over available conventional methods.

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“…Initially, rapid radiometric procedures were used to detect the presence of microorganisms (28) or growth of microorganisms (4,5,9,14,15,26,33). As early as 1956, Levin (14).…”

Section: Subcultures Of M Paratuberculosis Require 12 Weeks and Subcmentioning

confidence: 99%

“…As early as 1956, Levin (14). Since then, radiometric procedures have continued to be applied to clinical microbiology (12,15,27) including testing for suscep tibility to antimicrobials.…”

Section: Subcultures Of M Paratuberculosis Require 12 Weeks and Subcmentioning

confidence: 99%

“…During the last decade, a number of rapid radiometric assays have been developed to detect the presence and/or growth of microorganisms, or determine susceptibility to antimicrobials. The most common procedure has been to determine the susceptibility of microorganisms by 14 measuring the evolution of CO^ from microorganisms exposed to ^^C-labeled substrates (3,4,5,6,8,9,11,12,13,14,15,23,27,28,33,45). This procedure has become known as the CO^ procedure.…”

Section: Subcultures Of M Paratuberculosis Require 12 Weeks and Subcmentioning

confidence: 99%

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Development of rapid radiometric assays using the incorporation of [3H]uracil to determine susceptibility of slowly growing mycobacteria to antimicrobial agents

Hintz

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Automated Radiometric Detection of Bacteria in 2,967 Blood Cultures

Cited by 49 publications

References 0 publications

Comparison of the BACTEC System with Blind Subculture for the Detection of Bacteremia

Comparison of the BACTEC System with Blind Subculture for the Detection of Bacteremia

Evaluation of Radiometric System for Detecting Bacteremia

Development of rapid radiometric assays using the incorporation of [3H]uracil to determine susceptibility of slowly growing mycobacteria to antimicrobial agents

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