Automated simultaneous determination of total dissolved nitrogen and phosphorus in seawater by persulfate oxidation method

Yasui-Tamura, Saori; Hashihama, Fuminori; Ogawa, Hiroshi; Nishimura, Takashi; Kanda, Jun

doi:10.1016/j.talo.2020.100016

Cited by 14 publications

(2 citation statements)

References 21 publications

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“…These results are consistent with those of previous studies. [ 28,29 ] A relatively constant distribution of these elements in the vertical direction was observed in the sample obtained on January 20, 2021 (dashed line in Figure S5). This could have been caused by the convection of ocean water owing to violent tides.…”

Section: Resultsmentioning

confidence: 84%

Single‐cell genotyping of phytoplankton from ocean water by gel‐based cell manipulation

Yoshino

Mao

Maeda

et al. 2022

Biotechnology Journal

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A comprehensive understanding of phytoplankton diversity is valuable for assessing an environment of interest as phytoplankton are primary producers in various aquatic food webs. Microscopic analyses are useful for diversity assessment based on characteristic cell morphologies. However, phylogenetic classification based solely on morphology requires an extremely high level of expertise. The genetic approach is another option for evaluating phytoplankton diversity; however, it cannot reveal morphological information. To integrate these two approaches, an original technology was developed, that is referred to as microcavity array (MCA)/gel‐based cell manipulation (GCM). The model experiments using monocultures of various phytoplankton indicated that the efficiencies of cell recovery and isolation of single‐cell plankton were dependent on cell size and shape. Cells with widths larger than the cavity width showed high level of recovery and isolation efficiency. Subsequent whole‐genome amplification (WGA) of isolated single‐cell plankton provided a sufficient amount (≈30 μg) of WGA products for genetic analyses. Furthermore, it is showed that MCA/GCM could directly analyze phytoplankton in ocean water obtained from Suruga Bay, Japan, without any cumbersome pretreatment. These results indicate that MCA/GCM technology is a powerful tool for elucidating the phytoplankton diversity in marine environment.

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Section: Resultsmentioning

confidence: 84%

Single‐cell genotyping of phytoplankton from ocean water by gel‐based cell manipulation

Yoshino

Mao

Maeda

et al. 2022

Biotechnology Journal

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“…species (NO2 + NO3 + NH4). Total particulate phosphorus (TPP) and dissolved organic nitrogen samples were first oxidised by an acid-alkaline potassium persulfate digestion (Pujo-Pay & Raimbault, 1994;Yasui-Tamura et al, 2020) prior to analysis as inorganic phosphate. Briefly, TPP filters were submerged in a 3 mL solution of potassium persulfate solution (6% potassium persulfate (K2O8S2, Fisher Scientific CAS 7727-21-1), 3% boric acid (H3BO3, Sigma-Aldrich CAS 10043-35-3) in 1.05 M NaOH, and DON samples were mixed at a ratio of 4 mL sample to 1 mL persulfate solution, into glass vials with airtight caps (KIMBLE, PTFE-faced rubber, 15-415), then digested by autoclaving for 30 minutes at 120˚C, followed by 40x (TPP) or 3x (DON) dilution with artificial seawater prior to analysis as with dissolved inorganic nutrients.…”

Section: Dissolved Macronutrientsmentioning

confidence: 99%

Pathways and consequences of dissolved organic matter transformation in tropical coastal waters

Nichols¹

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To my wife, Delia, thank you for your support through all these years, and for being a wonderful mother to our son. Without you I would not be writing this today.To Patrick Martin, thank you for this opportunity, for all your patience, support and guidance. Learning from you has been an absolute privilege that I hope many other students get to experience. I will endeavor to carry your work ethic, dedication, and leadership example with me into the future.To my thesis committee advisors Alex Poulton and Adriana Lopes dos Santos, thank you for your advice and support. In particular, thank you to Alex for driving the final project to completion, and for your help with the field campaign on St. John's Island. To Yongli Zhou, thank you for your willingness to help no matter the project or timing. Without your selflessness the gaps in our data would be titanic. To Molly Moynihan, your ability to get things done has been an inspiration. Thank you for your support in all things coral. To Kristy Chang, Oon Yee and Chen Shuang, thank you for your tireless efforts in the lab and in the field, you've been a pleasure to work with over the years.In addition, I am grateful to the numerous researchers, faculty members and fellow students at ASE who have contributed both directly and indirectly to this thesis and to my PhD in general.

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Dissolved Organic Matter

Álvarez‐Salgado

Nieto‐Cid

Rossel

2022

Marine Analytical Chemistry

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Automated simultaneous determination of total dissolved nitrogen and phosphorus in seawater by persulfate oxidation method

Cited by 14 publications

References 21 publications

Single‐cell genotyping of phytoplankton from ocean water by gel‐based cell manipulation

Single‐cell genotyping of phytoplankton from ocean water by gel‐based cell manipulation

Pathways and consequences of dissolved organic matter transformation in tropical coastal waters

Dissolved Organic Matter

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