Automatic differentiation of human induced pluripotent stem cells toward synchronous neural networks on an arrayed monolayer of nanofiber membrane

Huang, Boxin; He, Yuqing; Rofaani, Elrade; Liang, Feng; Huang, Xiaochen; Shi, Jian; Wang, Li; Yamada, Ayako; Peng, Juan; Yong, Chen

doi:10.1016/j.actbio.2022.07.038

Cited by 4 publications

(1 citation statement)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The side-to-side distance of hexagonal holes of the micro-honeycomb and the frame width were 200 and 50 μm, respectively. Gelatin nanofibers were electrospun on the both sides of the micro-honeycomb substrate, which were coated with a 10 nm-thick gold layer by using a metal sputter (K6785X, Emitech), similar to our previous studies. − Gelatin from porcine skin was dissolved at 15% (w/v) in a 10:14:21 (v/v) mixture of Milli-Q water, ethyl acetate, and acetic acid, and electrospun at 0.2 mL·h –1 from a syringe needle onto the substrate placed on a collector, at a distance of 10 cm under a bias voltage of 11 kV. Subsequently, the gelatin nanofiber mesh layers were crosslinked with 0.2 M 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 0.2 M N -hydroxysuccinimide in ethanol for 4 h at room temperature, rinsed with ethanol, and dried.…”

Section: Experimental Sectionmentioning

confidence: 99%

Aerosol Jet-Printed High-Aspect Ratio Micro-Needle Electrode Arrays Applied for Human Cerebral Organoids and 3D Neurospheroid Networks

Zips,

Huang,

Hotte

et al. 2023

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

The human brain is a complex and poorly accessible organ. Thus, new tools are required for studying the neural function in a controllable environment that preserves multicellular interaction and neuronal wiring. In particular, high-throughput methods that alleviate the need for animal experiments are essential for future studies. Recent developments of induced pluripotent stem cell technologies have enabled in vitro modeling of the human brain by creating three-dimensional brain tissue mimic structures. To leverage these new technologies, a systematic and versatile approach for evaluating neuronal activity at larger tissue depths within the regime of tens to hundreds of micrometers is required. Here, we present an aerosol-jet- and inkjet-printing-based method to fabricate microelectrode arrays, equipped with high-aspect ratio μ-needle electrodes that penetrate 3D neural network assemblies. The arrays have been successfully applied for electrophysiological recordings on interconnected neurospheroids formed on an engineered substrate and on cerebral organoids, both derived from human induced pluripotent stem cells.

show abstract

Section: Experimental Sectionmentioning

confidence: 99%