Automatic preparation of protein crystals using laboratory robotics and automated visual inspection

Ward, Keith B.; Perozzo, Mary Ann; Zuk, William

doi:10.1016/0022-0248(88)90328-4

Cited by 31 publications

(12 citation statements)

References 5 publications

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“…A complete automated crystallization laboratory, combining these key ingredients, was developed by Ward and co-workers22. The system used syringe pumps to deliver solutions to a specially designed plate20 with image analysis of the results.…”

Section: Automating Laboratory Crystallization Processesmentioning

confidence: 99%

Lessons from high-throughput protein crystallization screening: 10 years of practical experience

Luft

Snell

DeTitta

2011

Expert Opinion on Drug Discovery

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Introduction X-ray crystallography provides the majority of our structural biological knowledge at a molecular level and in terms of pharmaceutical design is a valuable tool to accelerate discovery. It is the premier technique in the field, but its usefulness is significantly limited by the need to grow well-diffracting crystals. It is for this reason that high-throughput crystallization has become a key technology that has matured over the past 10 years through the field of structural genomics. Areas covered The authors describe their experiences in high-throughput crystallization screening in the context of structural genomics and the general biomedical community. They focus on the lessons learnt from the operation of a high-throughput crystallization screening laboratory, which to date has screened over 12,500 biological macromolecules. They also describe the approaches taken to maximize the success while minimizing the effort. Through this, the authors hope that the reader will gain an insight into the efficient design of a laboratory and protocols to accomplish high-throughput crystallization on a single-, multiuser-laboratory or industrial scale. Expert Opinion High-throughput crystallization screening is readily available but, despite the power of the crystallographic technique, getting crystals is still not a solved problem. High-throughput approaches can help when used skillfully; however, they still require human input in the detailed analysis and interpretation of results to be more successful.

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Section: Automating Laboratory Crystallization Processesmentioning

confidence: 99%

Lessons from high-throughput protein crystallization screening: 10 years of practical experience

Luft

Snell

DeTitta

2011

Expert Opinion on Drug Discovery

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“…Cox & Weber, 1987;Ward, Perozzo & Zuk, 1988;Chayen, Shaw Stewart, Maeder & Blow, 1990;Rubin, Talatous & Larson, 1991;Oldfield, Ceska & Brady, 1991;Chayen, Shaw Stewart & Blow, 1992;Soriano & FontecillaCamps, 1993;Sadaoui, Janin & Lewit-Bently, 1994;Chayen, Shaw Stewart & Baldock, 1994). The fundamental difference between the vapourdiffusion and microbatch methods is that diffusion methods are dynamic systems in which conditions are changing throughout the crystallization process and, hence, there is little control over the experiments once trials have been initiated.…”

Section: Comparison Of the Vapour-diffusion And Microbatch Methodsmentioning

confidence: 99%

Comparative Studies of Protein Crystallization by Vapour-Diffusion and Microbatch Techniques

Chayen

1998

Acta Crystallogr D Biol Crystallogr

105

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IntroductionNumerous reports have been published in the literature which describe the crystallization of macromolecules by a variety of crystallization methods, including the vapour-diffusion and microbatch techniques. This topical review compares the results of examples of proteins which were crystallized by both vapour-diffusion and microbatch methods. The inherent features of the vapourdiffusion and microbatch methods are discussed and some specific conditions where one method appears more favourable than the other are reported. Guidelines for the conversion of crystallization conditions from vapour diffusion to microbatch (and vice versa) are also presented.

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“…Several automated systems for the preparation of vapour-diffusion crystallization trials have been described (Cox & Weber, 1987;Kelders, Kalk, Gros & Hol, 1987;Ward, Perozzo & Zuk, 1988) and one for the automated preparation of micro-batch trials (Chayen, Shaw Stewart, Maeder & Blow, 1990). Three of these complete systems are now marketed as commercial products.…”

Section: Introductionmentioning

confidence: 99%

A flexible approach to automated protein crystallization

Oldfield¹,

Ceska²,

Brady³

1991

J Appl Cryst

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An automated system for protein crystallization trials is described. Similar to commercial systems in operation, this system is readily assembled at a far lower cost. The software runs on either a microcomputer or a VAX and is both versatile and readily modified for special requirements. The system is capable of pipetting polyethylene glycol solutions accurately. Root-mean-square deviations in the accuracy of pipetted well solutions were similar to results obtained manually. Factors influencing reproducibility are discussed.

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Automatic preparation of protein crystals using laboratory robotics and automated visual inspection

Cited by 31 publications

References 5 publications

Lessons from high-throughput protein crystallization screening: 10 years of practical experience

Lessons from high-throughput protein crystallization screening: 10 years of practical experience

Comparative Studies of Protein Crystallization by Vapour-Diffusion and Microbatch Techniques

A flexible approach to automated protein crystallization

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