Automatic tracking of labeled red blood cells in microchannels

Pinho, Diana; Lima, Rui; Pereira, Ana I.; Gayubo, Fernando

doi:10.1002/cnm.2526

Cited by 22 publications

(14 citation statements)

References 36 publications

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“…As a result, it is possible to obtain the CFL thickness. The procedure to track individual RBCs is presented and discussed in more detail elsewhere 7,19 .…”

Section: Image Analysismentioning

confidence: 99%

In vitro blood flow and cell-free layer in hyperbolic microchannels: Visualizations and measurements

et al. 2015

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Red blood cells (RBCs) in microchannels has tendency to undergo axial migration due to the parabolic velocity profile, which results in a high shear stress around wall that forces the RBC to move towards the centre induced by the tank treading motion of the RBC membrane. As a result there is a formation of a cell free layer (CFL) with extremely low concentration of cells. Based on this phenomenon, several works have proposed microfluidic designs to separate the suspending physiological fluid from whole in vitro blood. This study aims to characterize the CFL in hyperbolic-shaped microchannels to separate RBCs from plasma. For this purpose, we have investigated the effect of hyperbolic contractions on the CFL by using not only different Hencky strains but also varying the series of contractions. The results show that the hyperbolic contractions with a Hencky strain of 3 and higher, substantially increase the CFL downstream of the contraction region in contrast with the microchannels with a Hencky strain of 2, where the effect is insignificant. Although, the highest CFL thickness occur at microchannels with a Hencky strain of 3.6 and 4.2 the experiments have also shown that cells blockage are more likely to occur at this kind of microchannels. Hence, the most appropriate hyperbolic-shaped microchannels to separate RBCs from plasma is the one with a Hencky strain of 3.

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“…As a result, it is possible to obtain the CFL thickness. The procedure to track individual RBCs is presented and discussed in more detail elsewhere 7,19 .…”

Section: Image Analysismentioning

confidence: 99%

In vitro blood flow and cell-free layer in hyperbolic microchannels: Visualizations and measurements

et al. 2015

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“…To study the behavior of the CFL along complex geometries, image analysis plays an important role [18,19].…”

mentioning

confidence: 99%

Cell-Free Layer Measurements in a Network with Bifurcating Microchannels Using a Global Approach

Bento

Pinho

Pereira

et al. 2015

Operational Research

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One of the most interesting hemodynamic phenomenon observed in microchannels is the existence of a marginal cell-free layer (CFL) at regions adjacent to the wall. This is a well known phenomenon that occurs in simple glass capillaries and in vivo microvessels, but has never been investigated in detail in biomedical microdevices containing complex geometries. In the present chapter, in vitro blood flowing through bifurcating microchannels was studied, with the aim of characterizing the cell-free layer (CFL). For that three different videos with different hematocrit and flow rates were considered. All images were obtained by means of a high-speed video microscopy system and then processed in MatLab using the Image Processing toolbox. The numerical data was obtained automatically and analyzed by optimization techniques using the genetic algorithm approach. The results suggest that the CFL were formed in a similar way at the upper and lower regions in all bifurcations.

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“…However, the development of automatic tracking algorithms was out of the scope of this study. Pinho et al [28] proposed an automatic method able to compute multiple trajectories in a time effective manner and compared it to the manual tracking method used in the present study and they did not find significant differences. Hence, manual tracking was considered accurate enough for the purpose of this study.…”

Section: Transport Of Cells Inside the Scaffoldmentioning

confidence: 53%

µ-Particle tracking velocimetry and computational fluid dynamics study of cell seeding within a 3D porous scaffold

Marin

Grossi

Bianchi

et al. 2017

Journal of the Mechanical Behavior of Biomedical Materials

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Cell seeding of 3D scaffolds is a critical step in tissue engineering since the final tissue properties are related to the initial cell distribution and density within the scaffold unit. Perfusion systems can transport cells to the scaffold however; the fact that cells flow inside the scaffold pores does not guarantee cell deposition onto the scaffold substrate and cell attachment. The aim of this study was to investigate how fluid flow conditions modulate cell motion and deposition during perfusion. For such a purpose, a multiphase-based computational fluid dynamics (CFD) model was developed in conjunction with particle tracking velocimetry experiments (PTV) which for the first time were applied to observe cell seeding inside a 3D scaffold. CFD and PTV results showed the strong effect of gravity for lower flow rates leading to cell sedimentation and poor transport of cells to the scaffold. Higher flow rates help overcome the effect of gravity so more cells travelling inside the scaffold were found. Nonetheless, fluid flow drags cells along the fluid streamlines without intercepting the scaffold substrate. As a consequence, if cells do not deposit into the scaffold substrate, cell adhesion cannot occur. Therefore, cell-scaffold interception should be promoted and the present computational model which predicts the effect of gravity and fluid drag on cells trajectories could serve to optimise bioreactors and enhance cell seeding efficiency.

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Automatic tracking of labeled red blood cells in microchannels

Cited by 22 publications

References 36 publications

In vitro blood flow and cell-free layer in hyperbolic microchannels: Visualizations and measurements

In vitro blood flow and cell-free layer in hyperbolic microchannels: Visualizations and measurements

Cell-Free Layer Measurements in a Network with Bifurcating Microchannels Using a Global Approach

µ-Particle tracking velocimetry and computational fluid dynamics study of cell seeding within a 3D porous scaffold

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